Molecular marker closely interlocked with anti-anthracnose gene locus of common beans and detection method of molecular marker

A technology for anthracnose and kidney bean, which is applied in the field of molecular markers and its detection, can solve the problems of lack of molecular markers for disease-resistant breeding, affect the quality and yield of common kidney beans, and harm kidney beans, etc., so as to improve efficiency, save planting costs, and be economical. benefit effect

Inactive Publication Date: 2015-01-28
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As the main miscellaneous grain crop in my country, common bean has a wide range of planting areas, but it is often harmed by bean anthracnose in production. It is especially serious in a cool and humid environment, and the yield is reduced by 30% to 90%. In the popular year, the plants wilt and die, which seriously affects the quality and yield of common beans
So far, 20 anthracnose genes or QTLs have been mapped, but there is a lack of molecular markers that can be effectively used in disease resistance breeding

Method used

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  • Molecular marker closely interlocked with anti-anthracnose gene locus of common beans and detection method of molecular marker
  • Molecular marker closely interlocked with anti-anthracnose gene locus of common beans and detection method of molecular marker
  • Molecular marker closely interlocked with anti-anthracnose gene locus of common beans and detection method of molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, the acquisition of molecular marker SNP+In / Del / Pro7

[0025] 1. Test materials

[0026] Common bean germplasm red kidney bean with high anthracnose resistance, unified number: F00002322; anthracnose-susceptible germplasm Jingdou, unified number: F00000777; and its hybrid F 2:3 group. The above materials are all from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, in the document "Mingli Chen, Jing Wu, Lanfeng Wang, Xiaoyan Zhang, Matthew W. Blair, Jizeng Jia, Shumin Wang. Development of mapped simple sequence repeat markers from common bean Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation. Molecular breeding 2014 33:489-496 "disclosed.

[0027] 2. Identification of common bean resistance to anthracnose

[0028] For parents F00002322, F00000777 and 1,092 F 2:3 Family (each F 2:3 Take 30 seeds in the family) after the plant is sown, and when the three compound leaves of the plant ar...

Embodiment 2

[0040] Embodiment 2, verification of molecular marker SNP+In / Del / Pro7

[0041] The results of resistance identification showed that common bean Michelite, uniform number: F00005600, from the Institute of Crop Science, Chinese Academy of Agricultural Sciences, showed high susceptibility to anthracnose race 81. In this study, a hybrid combination was constructed using the highly resistant anthracnose variety F00002322 and the highly susceptible variety F00005600. 2 The consistency of the resistance phenotype and genotype of the individual plants in the isolated population was further verified by the marker SNP+In / Del / Pro7. Specific steps are as follows:

[0042] 1. Combining F00002322×F00005600 of common bean hybrids 2 Sow a single seed in a nutrient bowl, and when the three compound leaves are fully expanded, collect a three compound leaf to extract genomic DNA. The next day, the plants are inoculated with Bean anthracnose, cultured in a suitable environment, and the plant ph...

Embodiment 3

[0047] Example 3, Molecular Marker SNP+In / Del / Pro7 Marker Genotype Analysis of Common Kidney Bean Resistant Materials

[0048] The 10 disease-resistant resources and 8 susceptible resources used in this test were provided by the Institute of Crop Science, Chinese Academy of Agricultural Sciences. Academy of Sciences, 2008." Publicized.

[0049] The calculation method of the disease index and the method of dividing the resistance of kidney beans to anthracnose according to the disease index refer to the literature "Wang Kun. Molecular markers and localization of common kidney bean resistance genes. Chinese Academy of Agricultural Sciences, 2008." method.

[0050] According to the investigation result of single plant, utilize formula DI=∑(Ci×Ni) / 9N to calculate disease index, wherein DI is disease index; 2 The investigation standard of disease condition of single plant is consistent; Ni is the number of plants at each level; N is the total number of plants.

[0051] According...

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Abstract

The invention discloses a molecular marker closely interlocked with an anti-anthracnose gene locus of common beans and a detection method of the molecular marker. The molecular marker consists of DNA molecules shown by SEQ ID No.1 and SEQ ID No.2. Results show that by adopting the molecular marker to perform PCR amplification on DNA of a genome of a common bean to be detected, whether the common bean to be detected can resist bean anthracnose or not can be effectively detected according to the size of an amplification product. By adopting the method, the process of seed selection of new anti-anthracnose species can be accelerated, the efficiency of breeding for disease resistance can be improved, environmental pollution caused by pesticide control of anthracnose can be reduced, and meanwhile, the planting cost is lowered, so that the method has obvious economic and ecological benefits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker closely linked with an anthracnose resistance gene locus of common bean and a detection method thereof. Background technique [0002] Common bean (Phaseolus vulgaris L.) is one of the most important edible legumes in the world. It is rich in protein, starch, minerals and multivitamins. As the main miscellaneous grain crop in my country, common bean has a wide range of planting areas, but it is often harmed by bean anthracnose in production. It is especially serious under the cool and humid environment, and the yield is reduced by 30% to 90%. In the popular year, the plants wilt and die, which seriously affects the quality and yield of common kidney beans. So far, 20 anthracnose genes or QTLs have been mapped, but there is a lack of molecular markers that can be effectively used in disease resistance breeding. Therefore, discovering disease-resistant resources, dev...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王述民陈明丽武晶王兰芬朱振东
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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