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Making method and application of TiO2-CdSe nanocomposite photoelectric biosensor

A technology of nanocomposite materials and biosensors, which is applied in the analysis of materials, material analysis through optical means, material analysis through electromagnetic means, etc., can solve the problems of little application value, achieve small quantum size effect, and fast electrode response , good sensitivity and reproducibility

Inactive Publication Date: 2015-01-28
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Looking at the development of aptasensors, it can be found that most research groups focus on short-strand DNA (20-60bp) for DNA sensors, and most of these DNAs are designed according to certain rules in advance according to needs, and then by Synthesized by biological companies, but the DNA fragment does not actually exist, and the application value is not great

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A kind of TiO 2 -Preparation method of CdSe nanocomposite photoelectric biosensor

[0034] (1) A 1.0 cm×2.5 cm rectangular ITO conductive glass was ultrasonically cleaned with acetone, ultrapure water, and ethanol for 30 min, then dried with pure nitrogen, and used as a working electrode, a platinum wire as a counter electrode, and a saturated reference electrode Calomel electrode, with a scan rate of 0.05~0.2 V / s, in the potential range of -1.5~2.5 V, in the presence of 5×10 -3 mol / L L-lysine, PBS with a pH of 6.0, use cyclic voltammetry to electropolymerize L-lysine on the surface of the working electrode to form a layer of poly-L-lysine, and store in a vacuum desiccator ;

[0035] (2) Drop-coat 2 μL of TiO 2 - Solid CdSe complex or 2 μL of TiO 2 - Liquid CdSe complex to (1) the surface of the modified working electrode, and dry in a refrigerator at 4 °C;

[0036] (3) Continue to drip-coat 10 μL, 5 μg / mL upstream primer of target DNA or 10 μL, 10 μg / ...

Embodiment 2

[0038] Example 2 A kind of TiO 2 -Preparation method of CdSe nanocomposite photoelectric biosensor

[0039](1) A 1.0 cm×2.5 cm rectangular ITO conductive glass was ultrasonically cleaned with acetone, ultrapure water, and ethanol for 30 min, then dried with pure nitrogen, and used as a working electrode, a platinum wire as a counter electrode, and a saturated reference electrode Calomel electrode, with a scan rate of 0.05~0.2 V / s, in the potential range of -1.5~2.5 V, in the presence of 5×10 -3 In mol / L L-lysine and PBS with a pH of 7.4, use cyclic voltammetry to electropolymerize L-lysine on the surface of the working electrode to form a layer of poly-L-lysine, and store it in a vacuum desiccator ;

[0040] (2) Drop-coat 10 μL of TiO 2 - Solid CdSe complex or 10 μL of TiO 2 - Liquid CdSe complex to (1) the surface of the modified working electrode, and dry in a refrigerator at 4 °C;

[0041] (3) Continue to drip-coat 15 μL, 25 μg / mL upstream primer of target DNA or 1...

Embodiment 3

[0043] Example 3 A kind of TiO 2 -Preparation method of CdSe nanocomposite photoelectric biosensor

[0044] (1) A 1.0 cm×2.5 cm rectangular ITO conductive glass was ultrasonically cleaned with acetone, ultrapure water, and ethanol for 30 min, then dried with pure nitrogen, and used as a working electrode, a platinum wire as a counter electrode, and a saturated reference electrode Calomel electrode, with a scan rate of 0.05~0.2 V / s, in the potential range of -1.5~2.5 V, in the presence of 5×10 -3 mol / L L-lysine, PBS with a pH of 9.0, use cyclic voltammetry to electropolymerize L-lysine on the surface of the working electrode to form a layer of poly-L-lysine, and store in a vacuum desiccator ;

[0045] (2) Drop-coat 18 μL of TiO 2 - Solid CdSe complex or 18 μL of TiO 2 - Liquid CdSe complex to (1) the surface of the modified working electrode, and dry in a refrigerator at 4 °C;

[0046] (3) Continue to drip-coat 20 μL, 50 μg / mL upstream primer of target DNA or 20 μL, 20...

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Abstract

The invention elates to a making method and an application of a TiO2-CdSe nanocomposite photoelectric biosensor, and belongs to the technical field of biological detection sensors. A TiO2-CdSe complex is used as a photoelectric beacon substance, and a CdSe quantum dot enhances the photoelectric performances of TiO2 due to its quantum size effect, dielectric effect and surface effect in order to realize the sensitive, specific and rapid detection of body tissue target DNA and its housekeeping gene. The sensor made in the invention has the advantages of simple apparatus, low cost, easy miniaturization and integration, and easy popularization.

Description

technical field [0001] The present invention relates to a kind of TiO 2 -CdSe nanocomposite photoelectric biosensor preparation method and its application, the sensor is used for the detection of biological tissue DNA, which belongs to the technical field of biosensing detection. Background technique [0002] Looking at the development of aptamer sensors, it can be found that the research of most research groups on DNA sensors mainly focuses on short-chain DNA (20-60bp), and most of these DNAs are designed according to certain rules in advance according to needs, and then by The biological company synthesizes it by contract, but the DNA fragment does not actually exist, and its application value is not great. If DNA biosensors are to be used for actual detection, the detected DNA should be real. This requires finding the DNA that needs to be detected and identified from practical problems, and then designing sensors according to needs and applying them in practice. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00G01N27/416
Inventor 庞雪辉闫涛魏琴朱宝存范大伟马洪敏
Owner UNIV OF JINAN
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