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Preservation method of liquid bacterial agent for sewage treatment

The technology of a liquid inoculum and a preservation method, which is applied in the preservation of microorganisms and other directions, can solve the problems such as the inability to preserve the inoculum on a large scale, and achieve the effects of reducing the operation process, the preservation cost, the high bacterial activity and the simple preservation method.

Active Publication Date: 2015-02-18
CNOOC TIANJIN CHEM RES & DESIGN INST +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the disadvantage that the existing traditional microbial preservation technology cannot achieve large-scale preservation of bacterial agents, and at the same time, there are few researches on liquid microbial agent storage technology at room temperature at the present stage, the present invention provides a method that is easy to operate, large in storage capacity, and highly active after preservation. High-efficiency and low-cost liquid bacterial agent preservation method, so as to provide necessary technical support for the commercialization and industrial application of liquid bacterial agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Degradation of oily wastewater before and after storage by commercially available oily wastewater degrading bacteria (the OBT deoiling bacteria from Bioform Biotechnology Co., Ltd. was used in this example).

[0020] The oily wastewater degrading bacteria were expanded and cultivated by a fermenter to prepare 10L of oily wastewater degrading bacteria liquid (bacteria quantity: 2.5×10 9 cfu / ml, using the plate counting method, the same below), and stored in two batches in 5L square small-mouth plastic buckets. Using the preservation technology described in the present invention, the preservation steps of the degrading bacterial liquid of oily wastewater are as follows: adjust the pH value of the bacterial liquid to 6 with 0.05mol / L phosphate buffer solution, feed nitrogen into the plastic bucket for aeration for 30 minutes, press 0.5‰ and 2 Ascorbic acid and potassium sorbate were added at a ratio of ‰ (m / v), sealed with liquid paraffin and stored at room temperature.

...

Embodiment 2

[0024] Comparison of the phenol-degrading activity of commercially available (OBT lysing bacteria from Biwofeng Biotechnology Co., Ltd.) before and after storage of phenol-degrading bacteria.

[0025] The phenol-degrading bacteria is expanded and cultivated by a fermenter, and 10L of bacterial liquid is prepared (bacteria quantity: 3.2×10 9 cfu / ml), and stored in two batches in 5L square small-mouth plastic buckets. Using the preservation technique described in the present invention, the preservation steps of the phenol-degrading bacterial liquid are as follows: adjust the pH of the bacterial liquid to 6.2 with 0.07mol / L citrate buffer solution, feed nitrogen into the plastic bucket for 20min, and press the ratio of 0.8‰ and 3‰ respectively Add ascorbic acid and potassium sorbate, cover with liquid paraffin and keep sealed at room temperature.

[0026] After preservation by the above method, a batch of preserved bacterial liquids were opened for counting when they were preser...

Embodiment 3

[0029] Degradation of ammonia nitrogen by commercially available nitrifying bacteria (the BZT nitrifying bacteria of BIOFORM Biotechnology Co., Ltd. was used in this example) before and after storage.

[0030] Mix the nitrifying bacteria into 10L bacterial solution (bacterial quantity 2.6×10 9 cfu / ml), and stored in two batches in 5L square small-mouth plastic buckets. Using the preservation technique described in the present invention, the preservation steps of nitrifying bacteria are as follows: use 0.1mol / L citrate buffer solution to adjust the pH of the bacterial solution to 6.5, feed nitrogen into the plastic bucket for 10 minutes, and add ascorbic acid in proportions of 1‰ and 4‰ respectively and potassium sorbate, covered with liquid paraffin and sealed at room temperature.

[0031] After being preserved by the above method, a batch of bacteria liquids were opened for counting when they were stored for 6 months and 12 months, and compared with newly prepared nitrifying...

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Abstract

The invention discloses a preservation method of a liquid bacterial agent for sewage treatment. The preservation method comprises the following main steps: 1) regulating the pH value of the liquid bacterial agent to 6-6.5 by using a buffer solution; 2) removing dissolved oxygen from the liquid bacterial agent by using nitrogen; 3) adding a deoxidizer and a bacterium inhibitor; 4) covering with liquid paraffin, and sealing for preserving. By adopting the preservation method, 12 months later, the viable bacteria amount and the degrading activity can respectively reach 65% and 75% above.

Description

technical field [0001] The invention belongs to the field of microbial water treatment, and in particular relates to a method for preserving functional strains used in the field of sewage treatment. Background technique [0002] At present, my country's environmental pollution is still very serious, and various pollution control processes and methods have emerged one after another, among which biological treatment is the mainstream technical means. In recent years, the number of microbial agents developed for the purpose of environmental protection and pollution prevention and The use of it can not only greatly shorten the start-up time of the treatment system and improve the treatment efficiency, but also has a good degradation effect on refractory toxic pollutants, and can improve the impact resistance of the treatment system. [0003] However, because the environmental microbial bacterial agent is a live bacterial preparation, it dies quickly and is not easy to preserve; a...

Claims

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Application Information

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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 雷太平顾锡慧官赟赟李立峰张艳芳滕厚开陈军
Owner CNOOC TIANJIN CHEM RES & DESIGN INST
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