Preservation method of liquid bacterial agent for sewage treatment

The technology of a liquid inoculum and a preservation method, which is applied in the preservation of microorganisms and other directions, can solve the problems such as the inability to preserve the inoculum on a large scale, and achieve the effects of reducing the operation process, the preservation cost, the high bacterial activity and the simple preservation method.

Active Publication Date: 2015-02-18
CNOOC TIANJIN CHEM RES & DESIGN INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the disadvantage that the existing traditional microbial preservation technology cannot achieve large-scale preservation of bacterial agents, and at the same time, there are few researches on liquid microbial agent storage technology at room temperature at the present stage, the present inven

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1:

[0019] The degradation of oily wastewater before and after storage by commercially available oily wastewater degrading bacteria (in this example, OBT degreasing bacteria from Biwofeng Biotechnology Co., Ltd.) is used.

[0020] Expanded cultivation of oily wastewater degrading bacteria through the fermentation tank to prepare 10L of oily wastewater degrading bacteria liquid (bacteria amount: 2.5×10 9 cfu / ml, using the plate counting method, the same below), stored in 5L square plastic buckets in two batches. Using the preservation technology of the present invention to preserve the oily wastewater degradation bacterial solution is as follows: adjust the pH of the bacterial solution to 6 with 0.05 mol / L phosphate buffer, and aeration with nitrogen gas for 30 minutes into the plastic barrel, press 0.5‰ and 2 Add ascorbic acid and potassium sorbate in a ratio of ‰(m / v), cover them with liquid paraffin, and store them in a sealed place at room temperature.

[0021] Af...

Example Embodiment

[0023] Example 2

[0024] Comparison of phenol degradation activity of phenol-degrading bacteria on the market (this example uses OBT lysis bacteria of Biwofeng Biotechnology Co., Ltd.) before and after storage.

[0025] Expand the culture of phenol-degrading bacteria through the fermentor to prepare 10L of bacteria liquid (bacteria amount: 3.2×10 9 cfu / ml), stored in 5L square plastic buckets in two batches. Use the preservation technology of the present invention to preserve the phenol-degrading bacterial solution as follows: adjust the pH of the bacterial solution to 6.2 with 0.07mol / L citrate buffer, and pour nitrogen into the plastic barrel for 20 minutes, respectively at the ratio of 0.8‰ and 3‰ Add ascorbic acid and potassium sorbate, cover with liquid paraffin and seal and store at room temperature.

[0026] After the above-mentioned method, the bacteria solution was stored for 6 months and 12 months, and a batch of stored bacteria solution was counted, and compared with the...

Example Embodiment

[0028] Example 3:

[0029] Commercially available nitrifying bacteria (the BZT nitrifying bacteria of Biwofeng Biotechnology Co., Ltd. used in this example) degrade ammonia before and after storage.

[0030] Mix nitrifying bacteria into 10L bacterial solution (bacteria amount 2.6×10 9 cfu / ml), stored in 5L square plastic buckets in two batches. The steps of using the preservation technology of the present invention to preserve nitrifying bacteria are as follows: adjust the pH of the bacterial solution to 6.5 with 0.1 mol / L citrate buffer, pour nitrogen into the plastic bucket for 10 minutes, and add ascorbic acid at a ratio of 1‰ and 4‰ respectively And potassium sorbate, covered with liquid paraffin and sealed and stored at room temperature.

[0031] After the above-mentioned method, the bacteria solution was stored for 6 months and 12 months, and a batch of stored bacteria solution was opened to count, and compared with the newly prepared nitrifying bacteria, the degradation exper...

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Abstract

The invention discloses a preservation method of a liquid bacterial agent for sewage treatment. The preservation method comprises the following main steps: 1) regulating the pH value of the liquid bacterial agent to 6-6.5 by using a buffer solution; 2) removing dissolved oxygen from the liquid bacterial agent by using nitrogen; 3) adding a deoxidizer and a bacterium inhibitor; 4) covering with liquid paraffin, and sealing for preserving. By adopting the preservation method, 12 months later, the viable bacteria amount and the degrading activity can respectively reach 65% and 75% above.

Description

technical field [0001] The invention belongs to the field of microbial water treatment, and in particular relates to a method for preserving functional strains used in the field of sewage treatment. Background technique [0002] At present, my country's environmental pollution is still very serious, and various pollution control processes and methods have emerged one after another, among which biological treatment is the mainstream technical means. In recent years, the number of microbial agents developed for the purpose of environmental protection and pollution prevention and The use of it can not only greatly shorten the start-up time of the treatment system and improve the treatment efficiency, but also has a good degradation effect on refractory toxic pollutants, and can improve the impact resistance of the treatment system. [0003] However, because the environmental microbial bacterial agent is a live bacterial preparation, it dies quickly and is not easy to preserve; a...

Claims

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Application Information

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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 雷太平顾锡慧官赟赟李立峰张艳芳滕厚开陈军
Owner CNOOC TIANJIN CHEM RES & DESIGN INST
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