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Indoterpene speradine F derived from Aspergillus oryzae and its application

A kind of use and compound technology, applied in the direction of microorganism-based methods, microorganisms, anti-tumor drugs, etc., can solve problems such as drugs that have not yet been seen, and achieve significant anti-tumor activity

Inactive Publication Date: 2016-06-29
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity, and there is no report on the anti-proliferation activity of the compound on HL-60 cells, so there is no drug related to it in the market

Method used

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  • Indoterpene speradine F derived from Aspergillus oryzae and its application
  • Indoterpene speradine F derived from Aspergillus oryzae and its application
  • Indoterpene speradine F derived from Aspergillus oryzae and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Fermentative production and separation and purification of the compound of embodiment 1

[0018] 1 Fermentation production

[0019] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, get Aspergillus oryzae (Aspergillusoryzae) IBPT-1 (preserved in China Typical Culture Collection Center on December 1, 2011, address: Wuhan University, Wuhan, and the preservation number is: CCTCC NO: M2011437). Appropriate amount, inoculated on PDA solid slant medium and cultivated in 28°C incubator for 4 days.

[0020] Take an appropriate amount of Aspergillus oryzae (Aspergillus oryzae) IBPT-1 cultured on a slant for 4 days, and inoculate it into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, K H 2 PO 4 0.5, MgSO 4 0.3, seawater constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, mycelium a...

Embodiment 2

[0028] The test of embodiment 2 in vitro antitumor activity

[0029] 1 Experimental samples and experimental methods

[0030] Preparation of the tested sample solution The test sample is the pure product of the compound separated and purified in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0031] Cell lines and subculture of cells Tumor cell lines were used, and tumor cells were subcultured in DMEM medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0032] Cell Proliferation Inhibitory Activity Test Method (MTT)

[0033] Take the tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated in 96-well cell culture plates at 200 μL per well, and kept in 5% CO at 37°C. 2 Incubate for 4 h in an incubator. Set 5 concentration gradients for each sample, set 3 p...

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Abstract

The invention relates to an indole terpene speradine F derived from aspergillus oryzae and application. The compound has the effect of inhibiting tumor cell proliferation, and has anti-tumor activity. The structural formula of the compound is shown in the specification. A fermentation product is acquired by performing fermentation culturing on aspergillusoryzae IBPT-1, and then the compound is obtained by performing separation purification on the fermentation product. Experiments confirm that the compound has relatively antitumor activity on human promyelocytic leukemia cell line HL-60, and is applicable to antitumor research by being used to prepare medicines inhibiting cell proliferation or antitumor medicines.

Description

technical field [0001] The invention relates to an indoterpene speradine F derived from Aspergillus oryzae and its application. Background technique [0002] Indoterpenes are a class of compounds with unique biological activity isolated from the secondary metabolites of fungi and bacteria. They have inhibitory activity on potassium ion channels, antitumor activity, selective and potent progesterone receptor competitive Activity of methicillin-resistant Staphylococcus aureus. Cyclopiazonic acid (CPA) alkaloids are an important branch of indoterpenoids, they usually contain three structural units: an indole ring, a hemiterpene and two acetic acid branches. So far, only 6 similar natural products have been found. [0003] The inventors have studied and learned that the fermentation of Aspergillus oryzae (Aspergillusoryzae) IBPT-1 (preserved in China Center for Typical Culture Collection on December 1, 2011, address: Wuhan University, preservation number is: CCTCCNO: M2011437)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D491/22C12P17/18A61P35/00C12R1/69
CPCC07D491/22C12P17/18
Inventor 陈立胡筱赵杨杨张其清
Owner FUZHOU UNIV
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