Preparation method of solid microbial inoculant

A technology of solid-state bacterial agent and solid-liquid ratio, which is applied in the preservation of microorganisms, etc., can solve the problems of easy contamination of miscellaneous bacteria and short storage time of bacteria, and achieve the effects of reducing the probability of bacterial infection, long storage time, and improving viability

Active Publication Date: 2015-02-25
CNOOC TIANJIN CHEM RES & DESIGN INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the defects and deficiencies in the prior art, the present invention provides a preparation method of solid bacterial agent, which can solve the problems of short preservation time of bacteria and easy contamination of miscellaneous bacteria

Method used

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  • Preparation method of solid microbial inoculant

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Experimental program
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Effect test

Embodiment 1

[0016] Embodiment 1: prepare solid bacterial agent

[0017] Preparation of the culture medium of the bacteria: add the oil-removing bacteria agent BDB-a (purchased from Nanyang Donghua Engineering Technology Co., Ltd.) into the LB medium and cultivate it for 16 hours. After the cultivation, the bacteria solution is concentrated by sedimentation. 4 times.

[0018] Preparation of solid bacterial agent:

[0019] (1) Sterilize the solid adsorption carrier at 121°C for 30 minutes before use;

[0020] (2) adjust the pH of the concentrated strain culture solution to 5.0, add 2wt‰ potassium sorbate, and mix well;

[0021] (3) According to the ratio of solid-liquid ratio (g / mL) of 1:3, add wheat bran and talcum powder to the above mixed solution, mix evenly, place on a shaker for 1 hour, and set the speed at 50rpm, so that the strains are fully Adsorbed on the carrier;

[0022] (4) The above-mentioned mixed sample is placed in a 35°C oven for constant temperature drying, and the mo...

Embodiment 2

[0025] Embodiment 2: prepare solid bacterial agent

[0026] Preparation of the culture medium of the bacteria: add the oil-removing bacteria agent BDB-a (purchased from Nanyang Donghua Engineering Technology Co., Ltd.) into the LB medium and cultivate it for 16 hours. After the cultivation, the bacteria solution is concentrated by centrifugation. 3 times.

[0027] Preparation of solid bacterial agent:

[0028] (1) Sterilize the solid adsorption carrier at 121°C for 30 minutes before use;

[0029] (2) adjust the pH of the concentrated strain culture solution to 4.0, add 3wt‰ sodium benzoate, and mix well;

[0030] (3) According to the solid-liquid ratio (g / mL) of 1:2, add oat bran and bagasse to the above mixed solution and mix evenly, put it on a shaker for 1 hour, and set the speed at 50rpm, so that the bacteria can be fully adsorbed on on the carrier;

[0031] (4) The above-mentioned mixed sample is placed in a 35°C oven for constant temperature drying, and the moisture ...

Embodiment 3

[0034] Embodiment 3: prepare solid bacterial agent

[0035] Preparation of the culture medium of the bacteria: add the oil-removing bacteria agent BDB-a (purchased from Nanyang Donghua Engineering Technology Co., Ltd.) into the LB medium and cultivate it for 16 hours. The multiple is 2 times.

[0036] Preparation of solid bacterial agent:

[0037] (1) Sterilize the solid adsorption carrier at 121°C for 30 minutes before use;

[0038] (2) adjust the pH of the concentrated strain culture solution to 3.0, add 4wt‰ potassium sorbate, and mix well;

[0039] (3) According to the ratio of solid to liquid (g / mL) of 1:1, add corn flour to the above mixture and mix evenly, put it on a shaker and shake it for 1 hour, and set the speed at 50rpm, so that the bacteria can be fully adsorbed on the carrier ;

[0040] (4) The above-mentioned mixed sample is placed in a 35°C oven for constant temperature drying, and the moisture content reaches 10wt%;

[0041] (5) Take 1 g of the solid mic...

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Abstract

The invention discloses a preparation method of a solid microbial inoculant. The method comprises the following steps: concentrating a strain culture solution, adding an inhibitor, uniformly mixing a certain amount of solid adsorbing carrier, drying the mixture, and adding a deoxidizer to obtain the solid microbial inoculant. The method can enhance the storage time and viability of the solid microbial inoculant and lower the preservation and transportation cost; and the inhibitor and iron powder deoxidizer are added to lower the microbiological contamination probability, prevent the residual organic matters of the strain culture medium from oxidation, lower the activity of the target strain to some extent and perform promotion functions on prolonging the storage time.

Description

technical field [0001] The invention belongs to the technical field of strain preservation, and in particular relates to a preparation method of a solid bacterial agent. Background technique [0002] Microbial strains are widely used in the treatment of agriculture, feed and industrial wastewater. If the prepared strain culture solution is not specially treated, it will face the problems of short storage time, low vigor and easy variation. At present, this problem is generally solved by reducing the biological activity. Commonly used techniques include cryopreservation, freeze-drying preservation, adding protective agents to the bacterial solution, and preparing solid bacterial agents. The equipment cost of freezing technology is relatively high, and it is suitable for small amount preservation. The method of adding protective agent has short storage time and the situation that the bacteria liquid is easy to deteriorate. Therefore, when there are many microbial bacterial...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 官赟赟顾锡慧雷太平张艳芳滕厚开陈军李立峰
Owner CNOOC TIANJIN CHEM RES & DESIGN INST
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