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Preparation method of zinc-enriched liquid fermentation product of phellinus linteus

A liquid fermentation, zinc-enriched yeast technology, applied in the field of bioengineering

Active Publication Date: 2015-02-25
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there are no corresponding reports at home and abroad on the method of using specific low-frequency magnetic fields and low-frequency ultrasonic waves to assist in improving the yield and activity of Phellinus fermented zinc-rich products

Method used

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  • Preparation method of zinc-enriched liquid fermentation product of phellinus linteus
  • Preparation method of zinc-enriched liquid fermentation product of phellinus linteus
  • Preparation method of zinc-enriched liquid fermentation product of phellinus linteus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Material preparation

[0050] Neutral protease (enzyme activity of 200,000 U / g), papain (enzyme activity of 1,000,000 U / g), pectinase (enzyme activity of 50,000 U / g), cellulase (enzyme activity of 30,000 U / g) U / g), provided by Guangxi Pangbo Biological Engineering Co., Ltd.

[0051] PDA plate medium: 200 g of potato, 20 g of glucose and 15 g of agar, dilute to 1000 mL with water, natural pH, and sterilize at 121° C. for 20 min.

[0052] Phellinus species preserved on the slanted surface was inoculated on the PDA plate medium, and activated and cultivated, the culture temperature was 25° C., and the culture time was 7 days to obtain the activated Phellinus species.

[0053] Preparation of Exogenous Additives:

[0054] a. Preparation of the enzymatic hydrolysate of Asparagus oleifera leaves: after washing the picked Astragalus vine leaves, vacuum freeze-drying, weigh 100 g, add 500 mL of water, grind into a slurry, sterilize at 95°C for 5 minutes to inactivate variou...

Embodiment 2

[0083] 1. Material preparation

[0084] Neutral protease (enzyme activity of 300,000 U / g), papain (enzyme activity of 2,000,000 U / g), pectinase (enzyme activity of 30,000 U / g), cellulase (enzyme activity of 20,000 U / g) U / g), provided by Guangxi Pangbo Biological Engineering Co., Ltd.

[0085] PDA plate medium: 200 g of potato, 20 g of glucose and 20 g of agar, dilute to 1000 mL with water, natural pH, and sterilize at 121° C. for 20 min.

[0086] Phellinus linteus strains preserved on the slanted surface were inoculated on PDA plate medium, and activated and cultured, the culture temperature was 22° C., and the culture time was 10 days to obtain the activated Phellinus linteus strains.

[0087] Preparation of Exogenous Additives:

[0088] a. Preparation of the enzymatic hydrolysate of Asparagus oleifera leaves: After cleaning the picked Astragalus vine leaves, vacuum freeze-drying, weigh 100 g, add 400 mL of water, grind into a slurry, and sterilize at 90°C for 8 minutes to ...

Embodiment 3

[0099] 1. Material preparation

[0100] Neutral protease (enzyme activity of 250,000 U / g), papain (enzyme activity of 1,500,000 U / g), pectinase (enzyme activity of 40,000 U / g), cellulase (enzyme activity of 20,000 U / g) U / g), provided by Guangxi Pangbo Biological Engineering Co., Ltd.

[0101] PDA plate medium, same as Example 1.

[0102] Phellinus linteus strains preserved on the slanted surface were inoculated on PDA plate medium, and activated and cultured, the culture temperature was 28° C., and the culture time was 5 days to obtain the activated Phellinus linteus strains.

[0103] Preparation of Exogenous Additives:

[0104]a. Preparation of the enzymatic hydrolysate of Asparagus oleifera leaves: After cleaning the picked Astragalus vine leaves, vacuum freeze-drying, weigh 100 g, add 600 mL of water, grind into a slurry, sterilize at 92°C for 6 minutes to inactivate various enzymes; Adjust the pH of the slurry to 4.0, add a composite enzyme composed of 1.86g cellulase a...

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Abstract

The invention discloses a preparation method of a zinc-enriched liquid fermentation product of phellinus linteus. The preparation method comprises the following steps: inoculating a phellinus linteus strain onto a liquid seed culture medium added with zinc-enriched yeast and a diaphragma juglandis extract, rapidly culturing with the help of a low-frequency ultrasound with a certain frequency and intensity, inoculating the phellinus linteus liquid strain onto a liquid fermentation culture medium added with zinc-enriched yeast and a plukenetia volubilis leave enzymolysis product and culturing in an ultra-low-frequency magnetic field with a certain frequency and intensity to obtain the zinc-enriched liquid fermentation product of phellinus linteus. According to the preparation method, by the physical-assisted synergistic fermentation of seed liquid and fermentation two-phase low-frequency ultrasound and ultra-low-frequency magnetic fields and the synergistic effect of exogenous additives such as the diaphragma juglandis extract and the plukenetia volubilis leave enzymolysis product, the contents and activities of organic zinc and zinc polysaccharide in final fermentation product of phellinus linteus are significantly increased.

Description

technical field [0001] The invention relates to the field of biological engineering, in particular to a preparation method of a zinc-rich Phellinus linteus liquid fermentation product. Background technique [0002] Phellinus igniarius belongs to the Basidiomycetes subphylum, Strataporia class, Pseudomonas rustaceae, and the genus Necroporus. Phellinus linteus extract has significant effects in clinical applications such as inhibiting cancer cell metastasis and preventing cancer recurrence after surgery. It is currently the most efficient medicinal fungus among the internationally recognized biological cancer drugs. As a large-scale medicinal fungus with the best anti-cancer effect currently internationally recognized, the research on Phellinus linteus on tumor inhibition mainly focuses on the pharmacologically active ingredient Phellinus linteus polysaccharide. [0003] Zinc is a kind of trace element. Its content in the human body and daily intake are very small, but it ca...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/04C12R1/645
Inventor 程俊文贺亮胡传久魏海龙付立忠李海波邹景泉
Owner ZHEJIANG FORESTRY ACAD
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