Preparation method of zinc-enriched liquid fermentation product of phellinus linteus
A liquid fermentation, zinc-enriched yeast technology, applied in the field of bioengineering
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Embodiment 1
[0049] 1. Material preparation
[0050] Neutral protease (enzyme activity of 200,000 U / g), papain (enzyme activity of 1,000,000 U / g), pectinase (enzyme activity of 50,000 U / g), cellulase (enzyme activity of 30,000 U / g) U / g), provided by Guangxi Pangbo Biological Engineering Co., Ltd.
[0051] PDA plate medium: 200 g of potato, 20 g of glucose and 15 g of agar, dilute to 1000 mL with water, natural pH, and sterilize at 121° C. for 20 min.
[0052] Phellinus species preserved on the slanted surface was inoculated on the PDA plate medium, and activated and cultivated, the culture temperature was 25° C., and the culture time was 7 days to obtain the activated Phellinus species.
[0053] Preparation of Exogenous Additives:
[0054] a. Preparation of the enzymatic hydrolysate of Asparagus oleifera leaves: after washing the picked Astragalus vine leaves, vacuum freeze-drying, weigh 100 g, add 500 mL of water, grind into a slurry, sterilize at 95°C for 5 minutes to inactivate variou...
Embodiment 2
[0083] 1. Material preparation
[0084] Neutral protease (enzyme activity of 300,000 U / g), papain (enzyme activity of 2,000,000 U / g), pectinase (enzyme activity of 30,000 U / g), cellulase (enzyme activity of 20,000 U / g) U / g), provided by Guangxi Pangbo Biological Engineering Co., Ltd.
[0085] PDA plate medium: 200 g of potato, 20 g of glucose and 20 g of agar, dilute to 1000 mL with water, natural pH, and sterilize at 121° C. for 20 min.
[0086] Phellinus linteus strains preserved on the slanted surface were inoculated on PDA plate medium, and activated and cultured, the culture temperature was 22° C., and the culture time was 10 days to obtain the activated Phellinus linteus strains.
[0087] Preparation of Exogenous Additives:
[0088] a. Preparation of the enzymatic hydrolysate of Asparagus oleifera leaves: After cleaning the picked Astragalus vine leaves, vacuum freeze-drying, weigh 100 g, add 400 mL of water, grind into a slurry, and sterilize at 90°C for 8 minutes to ...
Embodiment 3
[0099] 1. Material preparation
[0100] Neutral protease (enzyme activity of 250,000 U / g), papain (enzyme activity of 1,500,000 U / g), pectinase (enzyme activity of 40,000 U / g), cellulase (enzyme activity of 20,000 U / g) U / g), provided by Guangxi Pangbo Biological Engineering Co., Ltd.
[0101] PDA plate medium, same as Example 1.
[0102] Phellinus linteus strains preserved on the slanted surface were inoculated on PDA plate medium, and activated and cultured, the culture temperature was 28° C., and the culture time was 5 days to obtain the activated Phellinus linteus strains.
[0103] Preparation of Exogenous Additives:
[0104]a. Preparation of the enzymatic hydrolysate of Asparagus oleifera leaves: After cleaning the picked Astragalus vine leaves, vacuum freeze-drying, weigh 100 g, add 600 mL of water, grind into a slurry, sterilize at 92°C for 6 minutes to inactivate various enzymes; Adjust the pH of the slurry to 4.0, add a composite enzyme composed of 1.86g cellulase a...
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