Supercharge Your Innovation With Domain-Expert AI Agents!

Method for recycling yeast abandoned after erythritol production

A technology of erythritol and yeast, which is applied in the field of biochemical industry to achieve the effects of avoiding environmental pollution, reducing processing costs and environmental pollution risks, and saving raw material costs

Inactive Publication Date: 2015-03-04
SHANDONG FOOD & FERMENT IND RES & DESIGN INST
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The search found that the research and development direction of erythritol production is mainly focused on how to improve the yield of strain erythritol and the control of fermentation conditions, while the research on the comprehensive utilization of by-products such as waste yeast cells produced in production is rare to report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for recycling yeast abandoned after erythritol production
  • Method for recycling yeast abandoned after erythritol production
  • Method for recycling yeast abandoned after erythritol production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Centrifuge the yeast cell sludge obtained in the erythritol production fermentation broth thalline separation process, add water, and prepare a suspension with a yeast cell concentration of 13% in g / 100mL; or the thalline separation process The yeast cell suspension obtained by ceramic membrane filtration is adjusted to a suspension with a bacterial cell concentration of 13% in g / 100mL; standby;

[0025] (2) Add 1.6% solid NaCl to the yeast suspension prepared in step (1) in g / 100mL, fully stir and mix, then carry out a high-pressure homogenization under a pressure of 50MPa, and keep the temperature of the feed liquid in the homogenization process Not exceeding 50°C;

[0026] (3) Insulate and autolyze the homogenized feed solution at 50±2°C for 20 hours, and then centrifuge at 4500 rpm to obtain bacterial cell autolyzed clear liquid and autolyzed residue;

[0027] (4) concentrating the cell autolysate obtained in step (3) in a vacuum at 65°C until the solid content...

Embodiment 2

[0032]Get 50L of the ceramic membrane filtration interception solution (thalline cell dry matter concentration 12w / v%) produced in the erythritol production fermentation broth thallus separation process, add 1kg solid NaCl thereinto, carry out high-pressure homogenization after stirring and dissolving, homogeneous The pressure is 45MPa, the homogenization temperature is 50°C, the homogenized cell suspension is incubated at 50°C, stirred and autolyzed for 16 hours, and centrifuged to obtain the autolyzed supernatant and autolyzed residue respectively. The obtained autolyzed supernatant was vacuum concentrated at 60-65° C. to a solid content of 70%, and 5.05 kg of the concentrate (containing 1 kg of NaCl) was obtained. Add 25L 0.2mol / L freshly prepared NaOH solution to the autolyzed residue obtained by centrifugation, stir at 100°C for 1.5 hours, centrifuge, collect the supernatant separately (for pH adjustment in the erythritol fermentation process), and then add 25L 0.2 mol / L ...

Embodiment 3

[0034] Get 60L of ceramic membrane filtration interception solution (yeast cell dry matter concentration 12w / v%) produced during the sterilization process of erythritol production fermentation broth, add 1kg solid NaCl to it, stir and dissolve, then carry out high pressure homogenization, homogenization pressure 50MPa , the homogenization temperature is 50°C, the homogenized cell suspension is incubated at 50°C, stirred and autolyzed for 10 hours, and centrifuged to obtain the autolyzed supernatant and the autolyzed residue respectively. The obtained yeast autolysate suspension was vacuum-concentrated at 60-65° C. to a solid content of 70%, to obtain 5.25 kg of concentrated yeast autolysate (containing 1 kg of NaCl). Add 25L of NaOH solution to the autolyzed yeast residue obtained by centrifugation (the yeast autolyzed residue is treated with secondary alkali to separate lye), stirred at 95°C for 2.0 hours, centrifuged, and the supernatant was collected separately (used for pH ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for recycling yeast abandoned after erythritol production. According to the method, bacterial cells separated out from an erythritol fermentation liquor are autolyzed under assistance, the autolysate is concentrated and used as an erythritol fermentation accessory instead of commercial yeast extract or yeast cream, the bacterial cell residue left after the autolysate is separated out is treated by use of an alkali liquor to obtain yeast cell wall polysaccharides, and the used alkali liquor is applied to regulating the pH in the erythritol fermentation process. The method for recycling the yeast abandoned after the erythritol production realizes complete utilization of the abandoned yeast cells in the erythritol production without causing new pollution to the process, and is especially suitable for popularization to erythritol production enterprises.

Description

technical field [0001] The invention relates to a method for reusing waste yeast cells in erythritol production, which belongs to the field of biochemical industry. Background technique [0002] Erythritol is a new type of sugar alcohol food sweetener, which has wide application in the production fields of functional food, beverage, special food, etc.; at present, its production mainly uses glucose as raw material and uses hyperosmotic yeast for fermentation transformation , the obtained fermented liquid undergoes cell separation, purification and impurity removal, and then crystallization and recrystallization to obtain the erythritol product. However, in the production of erythritol, waste yeast cells become one of the largest by-products (or waste), and about 500kg of wet yeast cells are produced for every ton of erythritol produced. The moisture content of the yeast after centrifugation is 75-85%, which is not easy to store. If it cannot be processed in time, it will sp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/18C08B37/00
CPCC12P7/18C08B37/00
Inventor 赵祥颖刘建军张立鹤田延军韩延雷杨丽萍张家祥
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com