Method for extracting high-activity hericium erinaceus polysaccharide

A technology of Hericium erinaceus polysaccharide and extraction method, which is applied in the field of extraction of high-activity Hericium erinaceus polysaccharide, can solve the problems of easy clogging and difficult removal of ultrafiltration membrane pores, and achieve the effects of high efficiency, good color and simple process

Inactive Publication Date: 2015-03-11
HARBIN MO MEDICAL BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent "Refinement and Purification Process of Fungal Polysaccharides" (200610012682.3) pointed out the method of purifying fungal polysaccharides, but for Hericium erinaceus polysaccharides, there are darker phenolic compounds connected to sugar chains, which are difficult to remove, and There are problems such as the interference of biological macromolecules such as cellulose and pectin, and the pores of ultrafiltration membranes are easily blocked.
Therefore, the Hericium erinaceus polysaccharide obtained by this method also has the defects that product color, efficiency, purity, etc. need to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Pulverization: Take dried Hericium erinaceus (obtained by natural air-drying of fresh Hericium erinaceus), pulverize with a pulverizer, and pass through a 60-mesh sieve to obtain dry Hericium erinaceus powder;

[0015] 2. Hot water leaching, suction filtration and concentration: Accurately weigh 6.25g of Hericium erinaceus dry powder, add distilled water with a mass ratio of 5 times, and extract in a constant temperature water bath at 75°C for 2 hours, and perform magnetic stirring while extracting , after leaching, the mixture is filtered with a suction filter, and the obtained filtrate is concentrated in a rotary evaporator to about 20 mL;

[0016] 3. Alcohol analysis and centrifugation: transfer the concentrated solution into a small Erlenmeyer flask, add ethanol with a concentration of 70% for alcohol analysis for 24 hours, so that the extracted high-activity Hericium erinaceus polysaccharide is completely precipitated, and the mixture is heated at 3500r / min...

Embodiment 2

[0021] 1, crushing: with embodiment 1;

[0022] 2. Hot water leaching, suction filtration and concentration: Accurately weigh 6.25g of Hericium erinaceus dry powder, add distilled water with a mass ratio of 10 times, and extract in a constant temperature water bath at 75°C for 4 hours, and perform magnetic stirring while extracting , after leaching, the mixture is filtered with a suction filter, and the obtained filtrate is concentrated in a rotary evaporator to about 20 mL;

[0023] 3. Alcohol analysis and centrifugation: transfer the concentrated solution into a small Erlenmeyer flask, add ethanol with a concentration of 80% for alcohol analysis for 24 hours, so that the extracted high-activity Hericium erinaceus polysaccharide is completely precipitated, and the mixture is heated at 3500r / min The crude product of Hericium erinaceus polysaccharide is obtained by centrifuging at a rotating speed;

[0024] 4, remove starch, remove protein: with embodiment 1;

[0025]...

Embodiment 3

[0028] 1, crushing: with embodiment 1;

[0029] 2. Hot water leaching, suction filtration and concentration: Accurately weigh 6.25g of Hericium erinaceus dry powder, add distilled water with a mass ratio of 15 times, and extract in a constant temperature water bath at 75°C for 6 hours, and perform magnetic stirring while extracting , after leaching, the mixture is filtered with a suction filter, and the obtained filtrate is concentrated in a rotary evaporator to about 20 mL;

[0030] 3. Alcohol analysis and centrifugation: transfer the concentrated solution into a small Erlenmeyer flask, add ethanol with a concentration of 90% for alcohol analysis for 24 hours, so that the extracted high-activity Hericium erinaceus polysaccharide is completely precipitated, and the mixture is heated at 3500r / min The crude product of Hericium erinaceus polysaccharide is obtained by centrifuging at a rotating speed;

[0031] 4, remove starch, remove protein: with embodiment 1;

[0032]...

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PUM

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Abstract

A method for extracting high-activity hericium erinaceus polysaccharide mainly comprising the following step: step 1, smashing; step 2, hot water extraction and suction filtration for concentration; step 3, alcohol precipitation and centrifugation; step 4, removing starch, protein and micromolecule substance, and alcohol precipitation; step 4, centrifugation and drying; step 6, detection. The method for extracting high-activity hericium erinaceus polysaccharide has the advantages that the extracted product is good in color, the efficiency and the purity are high, the process is simple, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides, in particular to a method for extracting polysaccharides from Hericium erinaceus with high activity. Background technique [0002] Hericium erinaceus, also known as Hericium erinaceus, Hericium erinaceus, Hericium erinaceus, Hedgehog, etc., is a large fungus, belonging to Basidiomycetes, Polyporaceae, Odontomycetes, and Hericium erinaceus, because its fruiting bodies are shaped like monkeys named after the head. The polysaccharide extracted from Hericium erinaceus is a glucan mixture whose main chain is connected by β-1,3 glycosidic bonds and branched chains are connected by β-1,6 glycosidic bonds. As a medicinal fungus, Hericium erinaceus has curative effects on diseases such as canceration of the digestive system, ulcers, gastric antrum inflammation, and chronic gastritis, and can improve the body's immunity. As an important class of biologically active substances, medicinal funga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 戴路
Owner HARBIN MO MEDICAL BIOLOGICAL TECH CO LTD
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