Efficient detoxification method for sweet cherry rootstocks

Abstract

The invention relates to an efficient detoxification method for sweet cherry rootstocks; the method comprises the steps: 1), utilizing sweet cherry rootstock leaf buds in a field as explants for tissue culture, sterilizing and culturing to obtain tissue culture seedlings; 2) subculture; 3) thermal treatment; 4) pre-culturing and loading; 5) vitrification; 6) freezing; 7) unfreezing in a water bath after freezing, performing recovery processing for once or twice and then inducing plantlets; 8) inducing plantlets and 9) virus detection. The viruses of virus-free seedlings Gisela VI obtained by detoxifying prunus necrotic ring-spot viruses of sweet cherry rootstocks in the combination of thermal treatment and super-freezing therapy can be removed completely; stem tips can be easily peeled off; and the efficient detoxification method has wide application values in promoting healthy and sustainable development of sweet cherry industries.

Description

Technical field: [0001] The invention relates to a method for detoxifying sweet cherry rootstock, in particular to a technology for removing necrotic ringspot virus from sweet cherry rootstock Prunus by using heat treatment combined with ultra-low temperature cryotherapy, and belongs to the technical field of fruit tree virus prevention and control. Background technique: [0002] Sweet cherry (Prunus avium L.) is currently the preferred tree species in my country to adjust the planting structure of fruit trees and increase farmers' income. Viral diseases have seriously affected the yield and quality of sweet cherries, and have become one of the main obstacles restricting the development of my country's sweet cherry industry. Prunus Necrotic Ringspot Virus (PNRSV) is the most common and serious virus in drupe fruit trees, which can lead to tree decline, yellowing or necrosis of leaves, delayed flowering, delayed fruit maturity or reduced yield. , Absolutely in severe cases. ...

AI Technical Summary

Problems solved by technology

Dai Hongyan et al. proved that micro-shoot tip culture of sweet cherry test tube seedlings could not effectively remove PNRSV

(2) Complicated operation and low efficiency

The traditional shoot tip culture requires the shoot tip to be stripped less than 0.5mm, which is difficult to operate and has low seedling efficiency

(3) Fu Hongqi adopted the method of embedding and dehydration combined with vitrification to freeze sweet cherry stem tips at ultra-low temperature. This method is relatively cumbersome and the cultivation period of plant materials is long

It is manifested that 30 days of low temperature acclimatization is required, and the stem tip material is preserved by embedding drying combined with vitrification method, and this method does not establish a frozen regeneration system for sweet cherry rootstock material

In addition, this method only explored the detoxification effect of sweet cherry with a single ultra-low temperature therapy, and the survival rate and detoxification rate were average.

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