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Eucommia ulmoides composition having myocardial protection effect and preparation of eucommia ulmoides composition

A myocardial protection and composition technology, applied in the field of eucommia component composition for the treatment of cardiovascular diseases, can solve the problems of single active ingredient, less application, and limited therapeutic effect of cardiovascular diseases

Active Publication Date: 2015-03-25
JIANGXI POZIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the current research shows that Eucommia ulmoides has a certain therapeutic effect on cardiovascular diseases, the products currently on the market of Eucommia ulmoides are mainly concentrated in the field of blood pressure reduction, and there are few applications in other fields
CN200710035744 discloses the application of a Eucommia lignan extract in anti-cardiovascular remodeling, its mechanism is still related to the antihypertensive effect of Eucommia (by regulating the renin-angiotensin RAS system), and the single effective Part (lignans), the active ingredient is relatively single, and the therapeutic effect on cardiovascular diseases is also extremely limited

Method used

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  • Eucommia ulmoides composition having myocardial protection effect and preparation of eucommia ulmoides composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 Preparation of effective parts of Eucommia ulmoides

[0018] Preparation of total flavonoids of Eucommia ulmoides: take 5.0kg of Eucommia ulmoides leaves coarse powder, prepare 40L of 45% ethanol, adjust the pH to 4 with concentrated hydrochloric acid, stir and extract twice at 60-70°C (extract with 25L ethanol solution for the first time) 2 hours, the second extraction with 15L ethanol solution for 1 hour), filtered, combined extracts, concentrated to 4L, and left to stand for 1.0 hour for centrifugation. Take the supernatant and add it to the treated macroporous resin column for saturated adsorption, then elute with water, 45% ethanol, and 65% ethanol successively. The flow rate of the adsorption extract is 0.5 retention volume / hour, and the flow rate during desorption is 2 1 retention volume / hour, discard the washing liquid, collect the 65% ethanol solution eluate, concentrate into a thick paste, and dry to obtain 0.34 kg of dry powder with a total flavo...

Embodiment 2

[0021] The experimental research of embodiment 2 Eucommia component compositions

[0022] Replication and administration method of animal myocardial ischemia model

[0023]Male SD rats, weighing 220±50g, 12 in each group. Sham operation group: fed with the same amount of aqueous solution; model group: fed with the same amount of aqueous solution; animals in each group were given the test drug (5ml / kg) by intragastric administration according to body weight. 1 time a day, a total of 7 administrations. Thirty minutes after the last administration, 1% sodium pentobarbital (50 mg / kg) was injected intraperitoneally for anesthesia, and the changes of electrocardiogram were continuously monitored. Bury the electrocardiogram electrode needle under the skin of the left lower limb and the right upper limb, find the great cardiac vein accompanying the left coronary artery between the left atrial appendage and the conus pulmonary artery, thread the thread with an ophthalmic needle 6-0 s...

Embodiment 3

[0043] Rat cardiomyocyte primary culture method: take out 25 SD suckling mice within 24 hours after birth, take out all the hearts, digest with digestive solution (0.125% trypsin + 0.05% type II collagenase), collect cells, and mix the collected cells The suspension was filtered through a 200-mesh stainless steel mesh to remove undigested tissue, inoculated into a culture bottle, and placed in a cell culture incubator (37°C, 95% O 2 +5%CO 2 ), and purified cardiomyocytes. After 2 hours, suck out the cell suspension that has not adhered to the wall, blow evenly and count, and adjust the density to 1×10 6 Implanted into a 24-well plate (500 μl per well) to distribute the cells evenly, add Brdu to 0.1 mmol / L, change the medium every 24 hours, and change the medium every 3-4 days thereafter. The cell growth was observed regularly under an inverted microscope. After 48 hours of culture, the cells were randomly divided into groups for experiments.

[0044] 37°C, 5% CO 2 cultured...

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Abstract

The invention belongs to the field of traditional Chinese medicine, and particularly relates to eucommia ulmoides composition for treating cardiovascular diseases. Ingredients of the composition are from traditional Chinese medicine eucommia ulmoides and specifically comprise, in percentage by weight, 10%-80% of total flavonoids of eucommia ulmoides, 10%-80% of iridoid of eucommia ulmoides and 10%-80% of lignans of eucommia ulmoides. Preferably, the composition comprises, in percentage by weight, 20%-40% of total flavonoids of eucommia ulmoides, 40%-60% of iridoid of eucommia ulmoides and 20%-50% of lignans of eucommia ulmoides. Single effective parts of eucommia ulmoides are combined, and the eucommia ulmoides composition with remarkable curative effect and clinic development prospect is obtained through adjustment of relevant weight ratios.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to an Eucommia component composition for treating cardiovascular diseases. Background technique [0002] Eucommia ulmoides is the dry bark of Eucommia ulmoides Oliv., a plant in the family Eucommia ulmoides. It is clinically used to treat liver and kidney insufficiency, waist and knee pain, habitual abortion of pregnancy, high blood pressure, etc., and is often compatible with psoralen and diapsis. [0003] Cardiovascular disease, also known as circulatory system disease, refers to the organs and tissues that transport blood in the human body, mainly including the heart and blood vessels, which can be subdivided into acute and chronic diseases, and are generally related to arteriosclerosis. Myocardial ischemia refers to a pathological state in which the blood perfusion of the heart is reduced, resulting in reduced oxygen supply to the heart, abnormal myocardi...

Claims

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Application Information

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IPC IPC(8): A61K36/46A61P9/00
CPCA61K36/46
Inventor 肖军平吴永忠陈梁康兴东毛金娣
Owner JIANGXI POZIN PHARMA
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