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A seed soaking solution for promoting germination of Cistanche seeds and preparation method thereof

A technology of Cistanche deserticola and seed soaking liquid, applied in botany equipment and methods, seed and rhizome treatment, chemicals for biological control, etc., can solve the problems of limited improvement of germination rate, etc. Lightweight, harmless to humans and animals

Active Publication Date: 2017-06-16
INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But the germination of above-mentioned Cistanche seeds is all only processed from the induction of chemical substances or phytohormones, and the improvement level of germination rate is limited.

Method used

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  • A seed soaking solution for promoting germination of Cistanche seeds and preparation method thereof
  • A seed soaking solution for promoting germination of Cistanche seeds and preparation method thereof
  • A seed soaking solution for promoting germination of Cistanche seeds and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Test material:

[0025] In 2011, desert Cistanche deserticola seeds (Cistanche deserticola Ma) were harvested in Jimsar County, and the seeds were screened by particle size classification, and the high-quality seeds of Cistanche deserticola Ma with a diameter greater than 0.7mm were selected for use.

[0026] 2. Drugs and instruments:

[0027] NaClO, fluridone, gibberellin, acetone, 95% ethanol, etc.; instruments are standard sieve, XTZ-EA (45X) stereo microscope (with micrometer), biochemical incubator, 9cm petri dish, tinfoil. Both fluridone and gibberellin were purchased by Xinjiang Furuike Biotechnology Co., Ltd., the product number of fluridone is 45511, and the product number of gibberellin is wi78755.

[0028] 3. Seed disinfection:

[0029] Cistanche deserticola seeds were soaked in 70% ethanol for 2 minutes, then disinfected with 2% sodium hypochlorite for 15 minutes, and finally rinsed with distilled water 3 times.

[0030] 4. Seed germination test:

[0...

Embodiment 2

[0047] 1. Test material:

[0048]In 2012, the seeds of Cistanche salsa (C.A.Mey.) G.Beck. were harvested in Habahe County, and the seeds were screened by particle size classification, and the high-quality seeds of Cistanche salsa with a diameter of 0.55-0.7mm were selected for use.

[0049] 2. Drugs and instruments:

[0050] NaClO, fluridone, gibberellin, acetone, 95% ethanol, etc.; instruments are standard sieve, XTZ-EA (45X) stereo microscope (with micrometer), biochemical incubator, 9cm petri dish, tinfoil. Both fluridone and gibberellin were purchased by Xinjiang Furuike Biotechnology Co., Ltd., the product number of fluridone is 45511, and the product number of gibberellin is wi78755.

[0051] 3. Seed disinfection:

[0052] Soak the seeds of Cistanche deserticola in 70% ethanol for 2 minutes, then disinfect them with 2% sodium hypochlorite for 15 minutes, and finally rinse them with distilled water 3 times.

[0053] 4. Seed germination test:

[0054] (1) Treatment 1: ...

Embodiment 3

[0070] 1. Test material:

[0071] The seeds of Cistanche tubulosa (schrenk) R.Wight were harvested in Hotan in 2009, and the seeds were screened by particle size classification, and high-quality seeds with a diameter greater than 0.7 mm were selected for future use.

[0072] 2. Drugs and instruments:

[0073] NaClO, fluridone, gibberellin, acetone, 95% ethanol, etc.; instruments are standard sieve, XTZ-EA (45X) stereo microscope (with micrometer), biochemical incubator, 9cm petri dish, tinfoil. Both fluridone and gibberellin were purchased by Xinjiang Furuike Biotechnology Co., Ltd., the product number of fluridone is 45511, and the product number of gibberellin is wi78755.

[0074] 3. Seed disinfection:

[0075] Soak Cistanche cistanche seeds in 70% ethanol for 2 minutes, then disinfect with 1% sodium hypochlorite for 10 minutes, and finally rinse with distilled water 3 times.

[0076] 4. Seed germination test:

[0077] (1) Treatment 1: Fluazinone was prepared with aceton...

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Abstract

The invention discloses a seed soaking solution for promoting the germination of Cistanche seeds, which is prepared by mixing equal volumes of a fluridone solution and a gibberellin solution, and provides a preparation method thereof. The beneficial effects of the present invention are: the seeds of Cistanche genus are soaked with the germination soaking solution for a certain period of time, and can reach a higher germination rate in a short period of time under suitable environmental conditions, break the dormancy of the seeds, promote the germination of the seeds, and Its germination rate is increased to more than 75%, which solves the problems of low germination rate and large amount of seeds in the artificial cultivation of Cistanche seeds; using the seed germination soaking liquid of the present invention has laid a foundation for artificial cultivation and inoculation of Cistanche, and the seeds of the present invention germinate The seed soaking solution can provide a new index for the quality determination of Cistanche deserticola seeds in commercial operation; the seed soaking solution has a good effect of improving seed germination, is low in cost, has light environmental pollution, is harmless to humans and animals, and is convenient for popularization and use.

Description

technical field [0001] The invention relates to the field of medicinal plant breeding, in particular to a seed soaking solution for promoting the germination of Cistanche seeds and a preparation method thereof. Background technique [0002] The seeds of Cistanche deserticola are dormant. To initiate seed germination, in addition to a long period of post-ripening process, there must be some kind of germination stimulating signal from the host root system. [0003] In recent years, the artificial cultivation area of ​​Cistanche has been expanding year by year, but the low germination rate and inoculation parasitism rate are the key problems that limit the production of Cistanche. The present invention artificially induces the in vitro germination of Cistanche deserticola, Cistanche saline, and Cistanche tuberosa seeds from the perspective of seed physiology and chemical substances, seeks suitable environmental conditions for seed germination, and innovates the artificial culti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/40A01C1/00A01P21/00A01N43/12
Inventor 刘永萍杨瑞鲁天平郭靖刘爱民莫双铭江晓珩张峰
Owner INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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