A kind of guided tissue regeneration film and its preparation method and application

A technology that guides tissue regeneration and double-layer membrane. It is used in chemical instruments and methods, medical science, prostheses, etc. It can solve the problems of inflammation, affecting the microstructure of fibers and the effect of use, and the degradation time is too fast. Tissue formation, favorable for cell growth, and wide clinical application

Active Publication Date: 2019-03-12
烟台蓝创生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although collagen membranes have excellent biocompatibility, they are expensive, have poor mechanical properties, and degrade too quickly. Although chemical crosslinking can improve their performance, there are also hidden dangers of residual cytotoxicity; in addition, polylactic acid The artificial membrane is hydrophobic, and the surface lacks functional groups for cell recognition factors or other bioactive molecules, resulting in a weak ability to bind to cells. The acidic products in the degradation process may cause inflammation, which limits its application.
In addition, most of the guided tissue regeneration membranes reported in the current literature or patents are designed in the form of a double-layer membrane, but the double-layer structure of the guided tissue regeneration membrane is prone to fracture and delamination. The layer is separated from the loose layer. In order to avoid this defect, complex processes such as mechanical pressing are required, which will affect the microstructure and use effect of the fiber.

Method used

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  • A kind of guided tissue regeneration film and its preparation method and application
  • A kind of guided tissue regeneration film and its preparation method and application
  • A kind of guided tissue regeneration film and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 A kind of preparation method of guided tissue regeneration film

[0047] 1. Dosing

[0048] Dissolve collagen and polylactic acid (PLA) in hexafluoroisopropanol (HFIP) according to the mass ratio of 1:9, and the ratio of material to liquid is 1:10, and stir until completely dissolved to obtain uniform spinning with a long degradation time Liquid(Z);

[0049] Dissolve collagen and polylactic acid (PLA) in hexafluoroisopropanol (HFIP) at a mass ratio of 9:1, with a material-to-liquid ratio of 1:10, and stir until completely dissolved to obtain uniform spinning with a short degradation time Liquid (Q).

[0050] 2. Spinning dense layer

[0051] Put the above spinning solution Z into the electrospinning syringe, adjust the rate of the micro-injection pump to 5ml / hour, the voltage of the electrospinning machine to 20KV, adjust the receiving distance of the receiving device to 20cm, and stop spinning after reaching a thickness of 0.1mm , quickly enter the next...

Embodiment 2

[0056] Embodiment 2 A kind of preparation method of guided tissue regeneration film

[0057] 1. Dosing

[0058] Dissolving gelatin and silk fibroin in trifluoroethanol at a mass ratio of 2:8, with a solid-liquid ratio of 1:20, stirring until completely dissolved to obtain a uniform spinning solution (Z) with a long degradation time;

[0059] Dissolve gelatin and silk fibroin in trifluoroethanol at a mass ratio of 8:2, with a solid-to-liquid ratio of 1:20, and stir until completely dissolved to obtain a uniform spinning solution (Q) with a short degradation time.

[0060] 2. Spinning dense layer

[0061] Put the above spinning solution Z into the electrospinning syringe, adjust the rate of the micro-injection pump to 0.5ml / hour, the voltage of the electrospinning machine to 10KV, adjust the receiving distance of the receiving device to 10cm, and stop spinning after reaching a thickness of 0.1mm. Silk, quickly enter the next step;

[0062] 3. Spinning potential loose layer

...

Embodiment 3

[0066] Embodiment 3 A kind of preparation method of guided tissue regeneration film

[0067] 1. Dosing

[0068] Chitosan and polylactic-glycolic acid (PLGA) are dissolved in formic acid according to the ratio of mass ratio 3:7, and the ratio of solid to liquid is 1:5, and stirred until completely dissolved to obtain a uniform spinning solution with a long degradation time (Z );

[0069] Chitosan and polylactic-glycolic acid (PLGA) are dissolved in formic acid according to the ratio of mass ratio 7:3, and the ratio of solid to liquid is 1:5, and stirred until completely dissolved to obtain a uniform spinning solution with a shorter degradation time (Q ).

[0070] 2. Spinning potential loose layer

[0071] The above-mentioned spinning solution Z and spinning solution Q are respectively packed in the electrospinning injectors Z and Q, the injection flow rate of the adjustment injector Z is 0.1ml / hour, and the ratio of the injection flow rate of the adjustment injector Q to the...

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Abstract

The invention discloses a guided tissue regeneration membrane. The membrane adopts a double-layer membrane structure and is characterized in that one layer is a compact layer prepared from biological fibers with longer degradation time, and the other layer is a potential loose layer prepared through mixing of biological fibers with different degradation time. After the potentialloose layer is implanted in a body, part of the biological fibers degrades quickly, so that the pore diameter of the membrane is increased, and ingrowth of cells is facilitated. The compact layer and the potential loose layer are connected through the same biological fibers with the longer degradation time, so that the double-layer membrane is free of splitting and fractures. The invention further discloses a preparation method of the guided tissue regeneration membrane as well as an application of the guided tissue regeneration membrane as a tissue repair product to tissue repair.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a membrane for guiding tissue regeneration, a preparation method thereof, and an application in tissue repair as a tissue repair product. Background technique [0002] The prototype of guided tissue regeneration technology began in 1982 when Nyman used a semi-permeable filter membrane to treat periodontal disease. In 1989, Dahlin et al. applied guided tissue regeneration technology to immediate dental implants, using it to guide bone regeneration around the implant, and achieved great success. Ideal bone regeneration effect. Now, guided tissue regeneration technology has been widely used in alveolar surgery, periodontics, oral implantology, orthopedics and other fields. [0003] At present, there are many kinds of guided tissue regeneration membranes available clinically, which are divided into two categories according to whether they can be absorbed: absorbable membranes and n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/44A61L27/54A61L27/58A61L27/60B32B5/14B32B5/26B32B23/10
Inventor 李东升张淑敏傅风华杨小平高久香谢泽平
Owner 烟台蓝创生物技术有限公司
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