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Selenous acidized beta-lactoglobulin, preparation method and application

A technology of lactoglobulin and selenium acidification, which is applied in the preparation method of peptides, plasma globulin/lactoglobulin, mammalian protein, etc., can solve the problem of dose dependence of antioxidant effect, narrow range of therapeutic dose and toxic dose of inorganic selenium, etc. problem, to achieve the effect of stable product properties, easy storage, and simple preparation process

Inactive Publication Date: 2015-04-01
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the range of therapeutic and toxic doses of inorganic selenium is very narrow, and the antioxidant effects of selenium often depend on higher doses

Method used

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  • Selenous acidized beta-lactoglobulin, preparation method and application
  • Selenous acidized beta-lactoglobulin, preparation method and application
  • Selenous acidized beta-lactoglobulin, preparation method and application

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preparation example Construction

[0040] ⑴ Selenite H 2 SeO 3 Preparation of: Selenium Dioxide SeO 2 with water H 2 O reacts to generate selenite H 2 SeO 3 ;

[0041] (2) Preparation of β-lactoglobulin liquid containing selenous acid: placing β-lactoglobulin in the excess selenous acid solution prepared in the above steps to obtain β-lactoglobulin liquid containing selenous acid;

[0042] (3) Introduction of selenite: place the above-mentioned β-lactoglobulin liquid containing selenous acid in an oxidation-reduction environment containing a strong oxidant, and react under vacuum at 35-45°C for 3-8 hours, and the selenium Acid is introduced into the arginine residue on β-lactoglobulin to generate selenized β-lactoglobulin;

[0043](4) Remove the ions in the reaction solution after the reaction is completed: use an ultrafiltration membrane with a molecular weight of 3000Da-7000Da to remove the strong oxidant with a smaller molecular weight in the previous step and the selenite that cannot be imported;

[...

Embodiment 1

[0046] A preparation method of selenized β-lactoglobulin, the steps are as follows:

[0047] (1) Take 5g of selenium dioxide and dissolve it in 50mL of water to make a selenous acid solution.

[0048] (2) Mix 10 g of β-lactoglobulin with the above-mentioned selenous acid solution to obtain a β-lactoglobulin liquid containing selenous acid.

[0049] (3) The oxidation-reduction substance in the above-mentioned reaction solution adopts 6% hydrogen peroxide, puts the above-mentioned reactants into a vacuum reactor, and stirs at 40 degrees for 5 hours under vacuum conditions to complete the introduction of selenite.

[0050] (4) Remove the ions in the reaction solution after the reaction is completed: use an ultrafiltration membrane with a molecular weight of 3000Da-7000Da to remove the strong oxidant with a smaller molecular weight in the previous step and the selenite that cannot be imported;

[0051] (5) Add 100 mL of ethanol to the reaction solution, centrifuge to obtain produ...

Embodiment 2

[0059] A preparation method of selenized β-lactoglobulin, the steps are as follows:

[0060] (1) Take 5g of selenium dioxide and dissolve it in 50mL of water to make a selenous acid solution.

[0061] (2) Mix 20 g of β-lactoglobulin with the above-mentioned selenous acid solution to obtain β-lactoglobulin liquid containing selenous acid.

[0062] (3) The oxidation-reduction substance in the above-mentioned reaction solution adopts 8% hydrogen peroxide, puts the above-mentioned reactants into a vacuum reactor, and stirs at 45 degrees for 7 hours under vacuum conditions to complete the introduction of selenite.

[0063] (4) Remove the ions in the reaction solution after the reaction is completed: use an ultrafiltration membrane with a molecular weight of 3000Da-7000Da to remove the strong oxidant with a smaller molecular weight in the previous step and the selenite that cannot be imported;

[0064] (5) Add 200mL of ethanol to the reaction solution, centrifuge to obtain the prod...

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Abstract

The invention relates to a synthesis and preparation method and an application of selenous acidized beta-lactoglobulin. Beta-lactoglobulin and SeO2 are taken as raw materials, selenous acid groups are introduced into beta-lactoglobulin with an oxidation-reduction method, and a novel organic selenium source is prepared. The preparationmethodcomprises the following steps: preparation of selenous acid; preparation ofa beta-lactoglobulinliquid containing selenous acid; introduction of selenous acid radical; filterationand centrifugation; freeze drying; and obtaining of a pure product selenous acidized beta-lactoglobulin. The obtained selenous acidized beta-lactoglobulin has high nutritive value, stable properties, and anticancer and anti-aging effects, and is an excellent organic selenium source. The method not only has greattheoretical significance on development of organic selenized protein, but also has great social practical significance on healthy aging.

Description

technical field [0001] The invention belongs to the interdisciplinary field of food science and technology, organic chemistry, and biopharmaceutical fields, and provides a selenized β-lactoglobulin, a synthesis preparation method and an application. Background technique [0002] Selenium is an essential trace element for living organisms and an important antioxidant in the body. Selenium affects free radical metabolism, antioxidant function, immune function, reproductive function and cell apoptosis by regulating selenoprotein or selenium enzyme (glutathione peroxidase and phospholipid hydrogen glutathione peroxidase) Wait. A large number of studies at home and abroad have shown that selenium is closely related to the aging of the body. Selenium, as a regulator of brain function, is involved in various neurodegenerations that accompany aging. [0003] Soil selenium is the main selenium source required by organisms. However, the abundance of selenium in the earth's crust is...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/107A23L1/305A61K8/64A61K38/17A61P37/04A23L33/17
CPCC07K14/4717A23L33/17A23V2002/00A61K8/64A61K38/00
Inventor 刘安军刘昊雨郑国强郑捷索佳丽徐晓明
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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