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Preparation method of sulfated gynostemma polysaccharide

A gynostemma polysaccharide and sulfated technology, which is applied in the field of preparation of sulfated gynostemma polysaccharides, can solve the problem of low immune activity of sulphated gynostemma polysaccharides, and achieve simple and controllable preparation process, high safety, and high immune activity Effect

Inactive Publication Date: 2016-06-01
NORTHWESTERN POLYTECHNICAL UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to overcome the deficiency that the sulfated Gynostemma polysaccharide prepared by the existing method has low immune activity, the present invention provides a preparation method of the sulfated Gynostemma polysaccharide

Method used

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  • Preparation method of sulfated gynostemma polysaccharide
  • Preparation method of sulfated gynostemma polysaccharide
  • Preparation method of sulfated gynostemma polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1, the extraction of Gynostemma pentaphyllum polysaccharide.

[0032]Take by weighing 500g of Gynostemma pentaphyllum dry plant (purchased in Xi'an Chinese Medicinal Materials Market), soak 48h with 95% ethanol in a large container, remove ethanol afterwards, use 95% ethanol 50 ℃ of reflux to extract 2 times on the rotary evaporator, each 2h , filter after the extraction, evaporate the ethanol, add ultrapure water to the dried product, reflux extraction in a large two-necked flask at 95°C for 3 times, each time for 3-4 hours, filter with double gauze after the end, and collect the filtrate , concentrated under reduced pressure at 50°C, centrifuged at 7000rpm at 20°C for 30min, took the supernatant, added 4 times the volume of 95% ethanol, precipitated overnight at 4°C, collected the precipitate, and centrifuged. The precipitate is washed with 95% ethanol, acetone and ether respectively, and finally freeze-dried to obtain Gynostemma pentaphyllum crude polysacc...

Embodiment 2

[0033] Embodiment 2, Gynostemma pentaphyllum crude polysaccharide depigmentation.

[0034] Adjust the pH value of 0.5% crude polysaccharide aqueous solution to 8.0 with concentrated ammonia water, and gradually add 30% H at 50°C under stirring. 2 o 2 , until the solution is light yellow, keep at constant temperature for 2h, use CH 3 The pH was adjusted to 7.0 with COOH, and concentrated under reduced pressure. Add 4 times the supernatant volume of 95% ethanol, overnight at 4°C, centrifuge (7000rpm, 15min), collect the precipitate, wash with absolute ethanol, acetone, ether, and freeze-dry. The refined polysaccharide was obtained and named as GPMP.

Embodiment 3

[0035] Example 3. Sulfation modification of Gynostemma pentaphyllum polysaccharide.

[0036] This method adopts sulfur trioxide-pyridine method to carry out sulfate modification on Gynostemma pentaphyllum polysaccharide. The effects of different reaction time and different proportions of reactants on the reaction process of sulfated Gynostemma pentaphyllum polysaccharide were determined, and the degree of substitution was used as an index.

[0037] Dissolve a certain amount of sulfur trioxide-pyridine complex with DMF in a beaker, then weigh 500 mg of Gynostemma pentaphyllum polysaccharide, add it to the system, and after stirring and dissolving, move the system into a three-necked flask with a thermometer and a stirring device, 60 ℃ water bath reaction for a certain period of time. After the reaction, cool to room temperature, adjust the pH of the system to 7.0 with 5% NaOH, then add 3 times the system of 95% ethanol, and stand overnight at 4°C. After the precipitate was pr...

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Abstract

The invention discloses a preparation method of sulfated gynostemma pentaphylla polysaccharide in order to solve the technical problem that the sulfated gynostemma pentaphylla polysaccharide prepared by an existing method is low in immunological competence. The preparation method comprises the following steps: adding sulfur trioxide-pyridine, an organic solvent N and N-dimethyl formamide into a three-mouth flask, completely dissolving and then adding gynostemma pentaphylla polysaccharide powder; arranging the system in a preheated water-bath device to have a constant-temperature reaction; afterwards cooling the system to room temperature; regulating the pH to 7.0 by using a NaOH liquid; adding ethanol for precipitation for one night; and centrifuging and drying in the second day to obtain the sulfated gynostemma pentaphylla polysaccharide. The preparation method disclosed by the invention is used for preparing a gynostemma pentaphylla polysaccharide product by adopting a three-factor three-level process and measuring the degree of substitution of the gynostemma pentaphylla polysaccharide product by adopting a barium chloride-gelatin turbidimetric method. The prepared sulfated gynostemma pentaphylla polysaccharide product is relatively high in immunological competence.

Description

technical field [0001] The invention relates to a preparation method of gynostemma polysaccharides, in particular to a preparation method of sulfated gynostemma polysaccharides. Background technique [0002] Gynostemma pentaphyllum, also known as paradise grass, super ginseng, gongluoguodi, rooting everywhere, aescin and aescin, is a characteristic natural resource in Shaanxi. Cold in nature, sweet in taste, beneficial to qi, calming the nerves, and lowering blood pressure, folks call it a "miraculous" "herb for longevity and longevity", and it is listed as the first of "precious Chinese medicinal materials" in the "Spark Project". The main active ingredients of Gynostemma pentaphyllum are polysaccharides, flavonoids, saponins and trace elements. Its effects are mainly to promote the metabolism of human fat substances and nourish human cells. At the same time, it has a good detoxification and anti-inflammatory effect. It is widely used in hyperlipidemia and fatty liver. , O...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
Inventor 尚晓娅白艳玲钞愈刘欢牛卫宁徐春兰钦传光高捷
Owner NORTHWESTERN POLYTECHNICAL UNIV
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