Direct analysis in real time (DART) method for rapid determination of artificially synthesized sweeteners in soft drink

A real-time direct analysis and artificial synthesis technology, applied in the field of food safety, to achieve the effect of rapid screening, high efficiency and simple operation

Inactive Publication Date: 2015-04-01
NANJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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False-positive results may occur w

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  • Direct analysis in real time (DART) method for rapid determination of artificially synthesized sweeteners in soft drink
  • Direct analysis in real time (DART) method for rapid determination of artificially synthesized sweeteners in soft drink
  • Direct analysis in real time (DART) method for rapid determination of artificially synthesized sweeteners in soft drink

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Embodiment 1

[0018] The mensuration of artificial sweetener in embodiment 1 apple cider vinegar

[0019] The apple cider vinegar sample was diluted with water, and 0.2uL apple cider vinegar solution was accurately pipetted on the sieve. After natural drying in the air, the sieve was placed in the gas outlet of the real-time direct analysis mass spectrometry ion source (Ionsense, USA) and the triple quadrupole mass spectrometer ( Thermo Tsq quantum access max) ion entrance, real-time direct analysis mass spectrometer ion source conditions are as follows: in negative ion mode, the working gas is high-purity helium, the injection speed is 0.4mm / s, the distance between the ion source and the transfer tube: 3.5cm, Ion source temperature: 300°C. The conditions of the triple quadrupole mass spectrometer are as follows: in negative ion mode, mass spectrometer mass analyzer voltage 4000V, capillary temperature: 320°C, lens voltage: 50V, mass-to-charge ratio scanning range is 50-300, and the ion con...

Embodiment 2

[0026] The mensuration of synthetic sweetener in embodiment 2 orange juice

[0027] Dilute the orange juice sample with water, accurately pipette 0.2uL of the orange juice solution, and place it on the screen. After natural drying in the air, place the screen between the gas outlet of the real-time direct analysis mass spectrometer ion source and the ion inlet of the triple quadrupole mass spectrometer for real-time direct analysis. The conditions of the mass spectrometry ion source are as follows: in negative ion mode, the working gas is high-purity helium, the injection speed is 0.4 mm / s, the distance between the ion source and the transfer tube: 3.5 cm, and the ion source temperature: 300 °C. The conditions of the triple quadrupole mass spectrometer are as follows: in negative ion mode, mass spectrometer mass analyzer voltage 4000V, capillary temperature: 320°C, lens voltage: 50V, mass-to-charge ratio scanning range is 50-300, and the ion conditions of the secondary mass spe...

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Abstract

The invention belongs to the field of food safety, and discloses a direct analysis in real time (DART) method for rapid determination of artificially synthesized sweeteners in a soft drink, which solves the problem that the conventional method requires complex sample pretreatment. The method particularly includes the following steps: a trace amount of a sample solution is accurately transferred to a screen; natural drying is carried out in the air; a DART ion source is combined with a triple quadrupole, so that the sample is directly analyzed through product ion scan under the negative ion mode; qualitative identification of the artificially synthesized sweeteners in the sample is carried out based on a comparison between tandem mass spectrums of a reference substance and the sample, and quantitative analysis is carried out through the external standard method. The limit of detection for the sweeteners determined through the method is 1ppm (parts per million), sample injection and analysis only need 5 minutes each time, and the linear relationship is good; acesulfame, sodium cyclamate and sodium saccharin in the soft drink can be determined simultaneously after simple dilution treatment of the sample; the detection method is sensitive, accurate, simple and convenient, and can achieve rapid screening of the artificially synthesized sweeteners in the soft drink.

Description

technical field [0001] The invention belongs to the field of food safety, and in particular relates to a method for simultaneously detecting multiple artificially synthesized sweeteners in soft drinks by using a DART ion source combined with a triple quadrupole mass spectrometer. Background technique [0002] Sweeteners refer to food additives that endow food with sweetness, improve food quality, and meet people's demand for food. Synthetic sweeteners are an important part of food additives. They do not metabolize and absorb in the human body, provide no or less calories, and will not cause dental caries, but their sweetness can reach dozens or even thousands of times that of sucrose , and the price is low and the dosage is small, it is favored by the food industry, and is widely used in food, drinks, pastry, candy and pharmaceutical factories. Sweeteners can be used alone or in combination. The sweeteners used in combination have higher sweetness and can cover up the taste...

Claims

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Application Information

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IPC IPC(8): G01N27/62
Inventor 李方实商丽娟李壹刘媛媛陈玲玲余童魁
Owner NANJING UNIV OF TECH
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