Method for preparing single-cell protein feed additive by utilizing ginkgo leaf processing wastes

A single-cell protein and feed additive technology, applied in microorganism-based methods, biochemical equipment and methods, animal feed, etc., to achieve the effect of increasing economic burden, retaining beneficial nutrients, and increasing application value

Inactive Publication Date: 2015-04-08

JIANGSU QIANYAOTANG GUOYI RES INST CO LTD

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AI-Extracted Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide a method for preparing a single-cell feed additive by fermenting complex microbial bacteria into a mixed medium composed of ginkgo leaf residue in the production and processing of ginkgo leaf extract and waste liquid absorb...

Abstract

The invention in particular discloses a method for preparing a single-cell protein feed additive by utilizing ginkgo leaf processing wastes. The method comprises the following steps: processing ginkgo leaf to extract the ginkgo leaf extract (GBE), drying the ginkgo leaf residues, grinding into ultra-fine powder, concentrating the outflow liquid waste subjected to macroporous resin adsorption in the ginkgo leaf extract preparation process into the concentrated solution, mixing at the weight ratio of the ginkgo leaf residue powder to the concentrated solution being (2-3) to 1, adding 15 weight percent of compound microbial fermentation agent for performing fermentation cultivation, carrying out Maillard reaction, drying at low temperature, pressing into granules, and packaging, thereby obtaining the single-cell protein feed additive product. According to the scheme in the invention, the technical gap of the domestic single-cell protein feed additive prepared by utilizing the ginkgo leaf processing wastes is filled, novel methods, process and technical routes are provided for recycling the waste residues and liquid waste in the ginkgo leaf processing waste processing production, and the feasibility is provided for industrialized operation.

Application Domain

FungiBacteria +2

Technology Topic

Fermentation starterBiotechnology +9

Examples

Experimental program(8)

Example Embodiment

[0045] Example 1

[0046] (1) Take out the ginkgo biloba residue after extracting the ginkgo leaf extract, drain the water, and then use a dryer to dry at 100-300°C (7-12 hours), and then crush it with a vibrating ultrafine grinder Make 500 mesh superfine powder to obtain ginkgo leaf residue powder;

[0047] (2) Collect the fresh waste liquid from the decoction of ginkgo biloba extract in the processing of ginkgo biloba leaf extract after the macroporous adsorption resin column adsorbs ginkgo flavonoids, and put it into a vacuum rotary evaporator at a temperature of 65°C and a vacuum of -0.1 Concentrate under the condition of MPa until the specific gravity of the waste liquid is 1.35 (measured at 40°C) to obtain a concentrated liquid;

[0048] (3) Weigh 255 kg of Ginkgo biloba dregs powder prepared in step (1) and 85 kg of concentrated liquid prepared in step (2), and place them in a tank mixer to fully stir them. Add appropriate amount of water to make the materials The water content is controlled at 55-63%, and the solid culture medium is made after stirring evenly;

[0049] (4) The solid culture medium prepared in step (3) is heated by a humid heat autoclave with a pressure of 0.7kg/cm 2 Sterilize at 115°C for 30 minutes;

[0050] (5) Take out 340 kg of solid culture medium after sterilization, let it cool to below 40℃, and insert 10.2 kg of Aspergillus niger, 10.2 kg of Streptococcus faecalis, 10.2 kg of Bacillus subtilis and 20.4 kg of Candida utilis Mix and mix the compound microbial starter, mix well, put it into a stainless steel plate with a thickness of between 4-6 cm, and send it to the fermentation chamber. Place the plate of the fermentation mixture to be cultured on the fermentation rack. Control the temperature of the fermentation room to 38℃, humidity to 72%, and indoor ventilation to 0.8m 3 /min, carry out solid culture fermentation for 60 hours to obtain fermentation culture material;

[0051] (6) After the cultivation and fermentation, seal the fermentation container and move it into a fermentation room with controllable high temperature and high humidity, set the temperature to 70°C and humidity to 90%, and leave it for natural fermentation for 10 days;

[0052] (7) Pour out the fermented material in step (6), and vacuum-dry it at 60°C until the water content of the material is less than 6%, to obtain single-cell protein material;

[0053] (8) The dried single-cell protein material is pulverized by a universal pulverizer, passed through a 100-mesh sieve, and then directly compressed into granules by a dry granulator. The compressed granules have a size of 60-80 mesh and are made of polyethylene. Packed in composite packaging bags, the finished product of single-cell protein feed additive is obtained, and the protein content of the product is 57.56%.

Example Embodiment

[0054] Example 2

[0055] (1) Take out the ginkgo biloba residue after decocting the ginkgo biloba extract, drain the water, and then use a hot-air drying oven to dry at 100-300°C (7-12 hours), and then crush it with a vibrating ultrafine grinder Make 800 mesh superfine powder to obtain ginkgo leaf residue powder;

[0056] (2) Collect the fresh waste liquid from the decoction of ginkgo biloba extract in the processing of ginkgo leaf extract through the macroporous adsorption resin column to absorb ginkgo flavonoids, and put it into the tubular evaporation concentrator at a temperature of 70°C and a vacuum degree of Concentrate under the condition of -0.085MPa to the specific gravity of the waste liquid to 1.4 (measured at 40°C) to obtain a concentrated liquid

[0057] (3) Weigh 204 kg of ginkgo leaf residue powder prepared in step (1) and 102 kg of concentrated liquid prepared in step (2), and place them in a tank mixer to fully stir them. Add appropriate amount of water to make the materials. The water content is controlled at 55-63%, and the solid culture medium is made after stirring evenly;

[0058] (4) The solid culture medium prepared in step (3) is heated by a humid heat autoclave with a pressure of 0.7kg/cm 2 Sterilize at 115°C for 30 minutes;

[0059] (5) Take out 306 kg of solid medium after sterilization, let it cool to below 40℃, and insert 9.18 kg of Aspergillus niger, 9.18 kg of Streptococcus faecalis, 9.18 kg of Bacillus cereus and 18.36 kg of Candida tropicalis. The compound microbial starter is mixed and mixed thoroughly, and put into the stainless steel plate. The thickness of the material layer is between 4-6 cm. Then, it is sent to the fermentation chamber. The material plate of the fermentation mixture to be cultivated is placed on the fermentation rack. The temperature of the fermentation room is 41℃, the humidity is 78%, and the indoor ventilation is 0.8m 3 /min, carry out solid culture fermentation for 52 hours to obtain fermentation culture material;

[0060] (6) After the cultivation and fermentation, seal the fermentation container and move it into a fermentation room with controllable high temperature and humidity, set the temperature at 80°C, humidity at 98%, and leave it for natural fermentation for 8 days;

[0061] (7) Pour out the fermented material in step (6), and vacuum-dry it at 60°C until the water content of the material is less than 6%, to obtain single-cell protein material;

[0062] (8) The dried single-cell protein material is pulverized by a universal pulverizer, passed through a 100-mesh sieve, and then directly compressed into granules by a dry granulator. The compressed granules have a size of 60-80 mesh and are made of polyethylene. Packed in composite packaging bags, the finished product of single-cell protein feed additive is obtained, and the protein content of the product is 58.4%.

Example Embodiment

[0063] Example 3

[0064] (1) Take out the ginkgo biloba residue after extracting the ginkgo leaf extract, drain the water, and then use a dryer to dry at 100-300°C (7-12 hours), and then use a universal crusher to crush into 100 Purpose fine powder to obtain ginkgo leaf residue powder;

[0065] (2) Collect the fresh waste liquid that flows out from the ginkgo leaf extract after the ginkgo leaf extract passes through the macroporous adsorption resin column to adsorb the ginkgo flavonoids, and puts it in the concentration tank at a temperature of 75°C and a vacuum of -0.09MPa Concentrate until the specific gravity of the waste liquid is 1.4 (measured at 40°C) to obtain a concentrated liquid;

[0066] (3) Weigh 212.5 kg of Ginkgo biloba residue powder prepared in step (1) and 85 kg of concentrated liquid prepared in step (2), and place them in a tank mixer to fully stir them. Add appropriate amount of water to make the materials The water content is controlled at 55-63%, and the solid culture medium is made after stirring evenly;

[0067] (4) The solid culture medium prepared in step (3) is heated by a humid heat autoclave with a pressure of 0.7kg/cm 2 Sterilize at 115°C for 35 minutes;

[0068] (5) Take out 297.5 kg of solid culture medium after sterilization, let it cool to below 40℃, and mix it with 8.925 kg of Aspergillus niger, 8.925 kg of Streptococcus faecalis, 8.925 kg of Bacillus coagulans and 17.85 kg of brewer’s yeast. The compound microbial starter is mixed thoroughly and evenly, put into the stainless steel plate, the thickness of the material layer is between 4-6 cm, and is sent to the fermentation chamber. Place the material plate of the mixture to be fermented on the fermentation rack to control the temperature of the fermentation chamber It is 39℃, humidity is 76%, indoor ventilation is 0.8m 3 /min, carry out solid culture fermentation for 55 hours to obtain fermentation culture material;

[0069] (6) After the cultivation and fermentation, seal the fermentation container and move it into a fermentation room with controllable high temperature and humidity, set the temperature at 75°C, humidity at 88%, and leave it for natural fermentation for 9 days;

[0070] (7) Pour out the fermented material in step (6), and vacuum-dry it at 60°C until the water content of the material is less than 6%, to obtain a single-cell protein material;

[0071] (8) The dried single-cell protein material is pulverized by a universal pulverizer, passed through a 100-mesh sieve, and then directly compressed into granules by a dry granulator. The compressed granules have a size of 60-80 mesh and are made of polyethylene. Packed in composite packaging bags, the finished product of single-cell protein feed additive is obtained, and the protein content of the product is 56.4%.

[0072] According to the technical scheme of the present invention, all of Aspergillus niger, Streptococcus faecalis, and Bacillus can be prepared by using existing methods, and a product with a dry cell powder and live bacteria concentration of 25 billion/g is obtained.

PUM

Property	Measurement	Unit
Particle size	60.0 ~ 80.0	mesh

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Classification and recommendation of technical efficacy words

increase economic burden
increase production cost

Method for extracting bone calcium through defatting fermentation and bone gla protein

ActiveCN102871122Aincrease economic burden

Owner:TIANJIN TIANSHI BIOLOGICAL DEV

Method for preparing single-cell protein feed additive by utilizing ginkgo leaf processing wastes

A single-cell protein and feed additive technology, applied in microorganism-based methods, biochemical equipment and methods, animal feed, etc., to achieve the effect of increasing economic burden, retaining beneficial nutrients, and increasing application value

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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