Pepsinogen I detection method and kit thereof

A pepsinogen and detection method technology, applied in the field of pepsinogen I dual-wavelength fluorescence immunochromatography detection method and its detection kit, can solve the problems of long detection time, high detection results, complicated operation process, etc., and achieve The effect of reducing the influence of impurities

Inactive Publication Date: 2015-04-08
江苏宏泰格尔生物医学工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this patent can only eliminate the interference of some turbid substances, but does not eliminate some non-specific binding, and the detection result may be higher than the actual concentration
The Chinese patent publication number is CN102323417

Method used

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  • Pepsinogen I detection method and kit thereof
  • Pepsinogen I detection method and kit thereof
  • Pepsinogen I detection method and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The dual-wavelength fluorescent immunochromatographic detection method of pepsinogen I of the present invention comprises the following steps:

[0036] 1) Preparation of immunochromatographic test strips: Coat the pepsinogen Ⅰ monoclonal antibody and chicken IgY on the detection line (T line) and control line (C line) of the chromatography test paper, respectively, using a film spraying machine. After drying for 1-5 hours, cut with a strip cutter to obtain immunochromatographic test strips with a width of 3-5 mm;

[0037] It should be noted that: a) Traditional qualitative immunochromatography uses coated goat anti-mouse antibody as the control line. With the increase of pepsinogen Ⅰ or some anti-mouse antibody blocking agents in the serum, the signal on the control line As it decreases, its signal value cannot be used for calculation, and the accuracy of the signal of the test line has no reference basis. The present invention adopts chicken IgY antibody and goat anti...

Embodiment 2

[0048] Such as Figures 1 to 2 As shown, the dual-wavelength fluorescent immunochromatographic detection kit of pepsinogen I of the present invention includes a kit body, a cryopreservation tube, and a diluent bottle, and the kit body includes a plastic liner 1 and is fixed on the plastic liner. The sample pad 2, the immunochromatography test strip 3 and the absorbent paper 6, the immunochromatography test strip is made of nitrocellulose membrane material, the sample pad and the absorbent paper are respectively lapped on both sides of the immunochromatography test strip, and the immunochromatography test strip is A detection line 4 and a control line 5 are set on the chromatography test strip, and the detection line and the control line are respectively coated with pepsinogen Ⅰ monoclonal antibody and chicken IgY; freeze-dried probes are stored in the cryopreservation tube, freeze-dried The probe is a freeze-dried probe prepared by reacting pepsinogen I monoclonal antibody and...

specific Embodiment

[0052]The present invention also provides a specific embodiment of dual-wavelength fluorescence immunochromatographic detection of pepsinogen I, which includes the following steps in sequence:

[0053] Step 1: Preparation of lyophilized probes

[0054] 1) Mix two fluorescent latex particle solutions with emission wavelengths of 550nm and 700nm respectively according to the volume ratio of 1:1. After mixing evenly, take 500 μl of mixed fluorescent latex particle solution (containing carboxyl groups) and use pH6.0 MES buffer After washing and centrifuging three times, the precipitate was diluted with pH6.0 MES buffer, and after adding 10mg EDC to mix well, the reaction was activated at room temperature for 30min. After centrifugation, the precipitate was washed three times with pH6.0 MES buffer, and then the precipitate was washed with pH6. Dilute with .0 MES buffer, add 125 μg pepsinogen Ⅰ monoclonal antibody, react at room temperature for 3 hours, add BSA to block, continue to...

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Abstract

The invention relates to a pepsinogen I detection method and a kit, and especially relates to a dual wavelength fluorescence immunity chromatography detection method of pepsinogen I and a detection kit thereof. The method comprises the following steps: 1)preparing an immunity chromatography test strip; 2)preparing a freeze-dried probe; 3)preparing a sample weak solution; and 4)examining the sample. The pepsinogen I detection method and the kit have the advantages of high sensitivity, high accuracy, simple operation and low cost.

Description

technical field [0001] The invention relates to a detection method and kit for pepsinogen I, in particular to a dual-wavelength fluorescent immunochromatographic detection method for pepsinogen I and a detection kit. Background technique [0002] Pepsinogen (PG), the precursor of pepsin, is a single-chain polymorphism with a molecular weight of 42,000 Da. It is divided into two subgroups according to its biochemical properties and immunogenicity. Components 1-5 have the same immunogenicity and are called PG Ⅰ, which are mainly secreted by the principal cells and mucus neck cells of the gastric gland, and most of them enter the gastric cavity; components 6-7 are called PG Ⅱ, which are secreted by the gastric fundus mucosa of the gastric body. The principal cells of the acid glands, the mucous neck cells of the oxyntic glands, the mucous cells of the cardia glands and the pyloric glands of the gastric antrum, and the Brunner glands of the upper duodenum. Under normal circumst...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/558G01N21/6486G01N33/531G01N33/68G01N2800/062
Inventor 张翼飞张鹏廖平璋张华
Owner 江苏宏泰格尔生物医学工程有限公司
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