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Method for separating cyanidin in nitraria sibirica pall through high-speed counter-current chromatography

A high-speed counter-current chromatography and cyanidin technology is applied in the field of high-speed counter-current chromatographic separation of cyanidin in white thorn, and achieves the effects of simple process, convenient operation and good repeatability

Active Publication Date: 2015-04-15
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, no one has used HSCCC technology to separate and purify cyanidin in Nitraria

Method used

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  • Method for separating cyanidin in nitraria sibirica pall through high-speed counter-current chromatography
  • Method for separating cyanidin in nitraria sibirica pall through high-speed counter-current chromatography
  • Method for separating cyanidin in nitraria sibirica pall through high-speed counter-current chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of crude extract

[0024] Add 100mL methanol (2% formic acid v / v) to 100g dried fruit of Nitraria tangutica and extract for 24h, repeating 3 times. After 3 times of extracts were combined, filter paper was used to remove insoluble matter, protein, and polysaccharides. After the filtrate was collected, the methanol was concentrated by a rotary evaporator, and the extract was redissolved in distilled water (2% formic acid) and applied to D-101 macroporous resin (50mm×800mm). The macroporous resin was pretreated with methanol and water. After loading the sample, flush the column with 3 times the column volume of distilled water to remove sugars, amino acids and other impurities. Then the column was flushed with methanol, and the effluent was collected, concentrated under reduced pressure, and dried.

[0025] 2. Preparation of cyanidin by high-speed countercurrent separation

[0026] Prepare a two-phase solvent system of methyl tert-butyl ether: n-butanol...

Embodiment 2

[0037] 1. Preparation of crude extract

[0038] 100g of dried Nitraria tangutica fruit was added to 100mL of methanol (2% formic acid) to extract for 24h, repeated 3 times. After 3 times of extracts were combined, filter paper was used to remove insoluble matter, protein, and polysaccharides. After the filtrate was collected, the rotary evaporator was concentrated to remove methanol, and the extract was reconstituted with distilled water (2.% formic acid) and applied to D-101 macroporous resin (50mm×800mm). The macroporous resin was pretreated with methanol and water. After loading the sample, flush the column with 3 times the column volume of distilled water to remove sugars, amino acids and other impurities. Then the column was flushed with methanol, and the effluent was collected, concentrated under reduced pressure, and dried.

[0039] 2. High-speed countercurrent separation

[0040] Prepare a two-phase solvent system of methyl tert-butyl ether: n-butanol: acetonitrile...

Embodiment 3

[0043] 1. Preparation of crude extract

[0044] 100g of dried Nitraria tangutica fruit was added to 100mL of methanol (2% formic acid) to extract for 24h, repeated 3 times. After 3 times of extracts were combined, filter paper was used to remove insoluble matter, protein, and polysaccharides. After the filtrate was collected, the methanol was concentrated by a rotary evaporator, and the extract was redissolved in distilled water (2% formic acid) and applied to D-101 macroporous resin (50mm×800mm). The macroporous resin was pretreated with methanol and water. After loading the sample, flush the column with 3 times the column volume of distilled water to remove sugars, amino acids and other impurities. Then the column was flushed with methanol, and the effluent was collected, concentrated under reduced pressure, and dried.

[0045] 2. High-speed countercurrent separation

[0046]Prepare a two-phase solvent system of methyl tert-butyl ether: n-butanol: acetonitrile: water (0....

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Abstract

The invention provides a method for separating cyanidin in nitraria sibirica pall through high-speed counter-current chromatography. With utilization of the high-speed counter-current chromatography and adopting of a reasonable solvent system, the high-purity cyanidin is obtained from nitraria sibirica pall; the method requires no use of a solid-phase carrier, has no irreversible adsorption, and separates and prepares cyanidin anthocyanin with no loss of samples, no pollution, high efficiency, speediness and low cost. The method has the advantages of simple process, convenient operation, safety, and good reproducibility, and can be used for industrialized production.

Description

technical field [0001] The invention relates to a method for separating cyanidin in Nitraria thorn by high-speed countercurrent chromatography. Background technique [0002] Tangut Nitraria is called Kami in the "Dictionary of Traditional Chinese Medicine". It is harvested when the fruit is ripe, dried and used as medicine, which has the effect of invigorating the spleen and stomach. Nourishes and strengthens, regulates menstruation and activates blood circulation, mainly treats diseases such as emaciation, deficiency of qi and blood, disharmony between spleen and stomach, indigestion, irregular menstruation, and low back pain due to deficiency and cold. According to the "Compendium of Materia Medica", Nitraria "smells pungent, cold, and non-toxic. It is mainly used to treat angina pectoris, carbuncle, ulceration and pus, and relieve pain. It can treat the deficiency of the husband, the essence of yang fistula will come out, nourish the kidney qi, and benefit the essence, et...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 丁晨旭张秋龙胡娜马涛李文聪索有瑞
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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