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Method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga

A technology of microalgae organisms and microalgae oil, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increasing process complexity, microalgae growth poisoning, microalgae damage, etc., and achieves high potential Economic and social benefits, reduced energy consumption, simple and fast processes

Inactive Publication Date: 2015-04-15
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the added flocculant is likely to cause damage to microalgae, and in the subsequent microalgae processing process, because the flocculant in the harvested microalgae must be extracted, the complexity of the process is increased.
In addition, when the concentration of some residual flocculants is too high, it will cause toxicity to the growth of microalgae, and the cost of some safe and non-toxic polysaccharide flocculants is relatively high
[0008] Air flotation is a relatively promising microalgae enrichment method, but this method requires reasonable air flotation separation equipment and separation process, and the separation effect is affected by many factors such as equipment structure and process conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The hydrogen-producing cyanobacterium A7119 and the oil-producing green algae Chlorella were cultivated in BGII medium. The algae solution was placed at 30°C and 30μmol photon m –2 the s –1 (Model MQ-100) light intensity, and through CO 2 :Air=2:98(v:v) mixed gas. Hydrogen-producing cyanobacteria and oleaginous green algae were cultured to end-logarithmic OD 750 When the value is 0.8, A7119 and Chlorella are co-induced to produce hydrogen and oil according to the volume ratio of 1:5. The method of co-induction is induction of auxotrophy; after the cultivation, use high-pressure gas chromatography to analyze the purity, quantify and collect the hydrogen gas, harvest the self-floating algae cells, and use the chloroform / methanol method to extract the oil in the microalgae cells. The hydrogenation reaction is carried out, and high-quality biodiesel whose main component is hydrocarbons is obtained after the reaction is completed.

Embodiment 2

[0031] The hydrogen-producing cyanobacterium A7119 and the oleaginous green algae cc124 were cultivated in BGII medium. The algae solution was placed at 30°C and 30μmol photon m –2 the s –1 (Model MQ-100) light intensity, and through CO 2 :Air=2:98(v:v) mixed gas. Hydrogen-producing cyanobacteria and oleaginous green algae were cultured to end-logarithmic OD 750 When the value is 0.95, A7119 and cc124 are co-induced to produce hydrogen and oil according to the volume ratio of 1:2. The method of co-induction is anaerobic induction. After the cultivation, the hydrogen gas is analyzed, quantified and collected by high-pressure gas chromatography, and the self-floating algae cells are harvested, and the oil in the microalgae cells is extracted by the chloroform / methanol method. The hydrogenation reaction is carried out, and high-quality biodiesel whose main component is hydrocarbons is obtained after the reaction is completed.

Embodiment 3

[0033] The hydrogen-producing cyanobacteria A7119 and the oleaginous green algae cc503 were cultivated in TAP medium. The algae solution was placed at 30°C and 30μmol photon m –2 the s –1 (Model MQ-100) light intensity, and through CO 2 :Air=2:98(v:v) mixed gas. Hydrogen-producing cyanobacteria and oleaginous green algae were cultured to end-logarithmic OD 750 When the value is 0.9, A7119 and cc503 are co-induced to produce hydrogen and oil according to the volume ratio of 10:1. The method of co-induction is dark induction. After the cultivation, the hydrogen gas is analyzed, quantified and collected by high-pressure gas chromatography, and the self-floating algae cells are harvested, and the oil in the microalgae cells is extracted by the chloroform / methanol method. The hydrogenation reaction is carried out, and high-quality biodiesel whose main component is hydrocarbons is obtained after the reaction.

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Abstract

The invention discloses a method for preparing microalgae biodiesel from oil-production green alga collected through self-floating of hydrogen-production blue alga. The method comprises the following steps: first, cells of hydrogen-production microalga and oil-production microalga are cultured; then the hydrogen-production glue-green alga and the oil-production green alga are subjected to co-induction regulation in a certain proportion for hydrogen production and oil production, the cells of the hydrogen-production microalga and the oil-production microalga are collected through self-floating, and the purity analysis and rationing of hydrogen and extraction of microalgae oil are conducted; finally, the hydrogen produced through microalgae photosynthesis is directly added to the microalgae oil for preparation of high-quality biodiesel. The oil-production green alga are collected through the phenomenon that the filiform nitrogen-fixing blue alga can float automatically during hydrogen production; compared with a traditional microalgae collection method, the method does not need addition of flocculants or ventilation, does not need aiding of other high-energy-consumption equipment either, and solves the problem that the microalgae collection cost is excessively high. Meanwhile, the high-quality biofuel can be obtained through reaction of hydrogen and oil produced by the alga, and the method has vital practical significance and economic efficiency.

Description

technical field [0001] The invention belongs to the technical field of bioenergy preparation, and in particular relates to a method for preparing microalgae biodiesel by self-floating hydrogen-producing cyanobacteria and harvesting oil-producing green algae. Background technique [0002] In today's society, the depletion of fossil energy, the scarcity of fresh water resources and the greenhouse effect seriously restrict the development of society and economy, and there is an urgent need to find new types of green energy that are efficient, clean and pollution-free. Microalgae, as a crop with fast growth, high oil production and less dependence on resources such as fresh water and arable land, has gradually attracted the attention of energy scientists and environmental scientists around the world. [0003] Harvesting algae cells is a key link in large-scale microalgae cultivation. Because microalgae cells are small (3-30 μm) and the concentration in the culture medium is ver...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/64C12R1/89C12R1/01
CPCY02E50/10
Inventor 李十中陈明王文睿
Owner TSINGHUA UNIV
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