Saury antioxidative peptide as well as separation and extraction method and application thereof

A technology of antioxidant peptides and extraction methods, applied in the fields of application, peptides, food preparation, etc., can solve problems such as waste of resources, achieve the effects of improving flavor, increasing utilization rate and added value, and improving food stability

Inactive Publication Date: 2015-04-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Saury protein is rich in a variety of amino acids with nutritional value. At present, these amino acids have not been fully utilized, resulting in a waste of resources

Method used

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  • Saury antioxidative peptide as well as separation and extraction method and application thereof
  • Saury antioxidative peptide as well as separation and extraction method and application thereof
  • Saury antioxidative peptide as well as separation and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Remove the head and viscera of the saury, wash the blood, and twist it into surimi, mix the surimi with water at a mass ratio of 1:2, add flavor protease, the addition amount is 1500U / g protein, at a pH of 6.5, Enzymolysis at 45°C for 4 hours; incubation at 85°C for 30 minutes, centrifugation at 1500g for 30 minutes, and supernatant to obtain saury protein hydrolyzates (PSPHs), with a degree of hydrolysis of 13.10%±0.72%;

[0033] (2) Use HP-20 macroporous adsorption resin to separate PSPHs. After loading the sample, use distilled water and gradient elution with volume fractions of 10%, 30%, 50% and 70% ethanol solution, and collect 30% ethanol solution for elution The components were freeze-dried to obtain peptide powder I;

[0034] (3) Separate the peptide powder I with a Sephadex G-25 Sephadex chromatographic column, elute with distilled water as the mobile phase, obtain three components in sequence according to the elution time, and collect the last eluted compo...

Embodiment 2

[0038] (1) Remove the head and viscera of the saury, clean the blood and twist it into surimi, mix the surimi with water at a mass ratio of 1:2, add trypsin, the addition amount is 500U / g protein, at a pH of 8.5, Enzymolysis at 55°C for 8 hours; incubation at 100°C for 10 minutes, centrifugation at 4000g for 10 minutes, and supernatant to obtain saury protein hydrolyzates (PSPHs), with a degree of hydrolysis of 15.58%±0.55%;

[0039] (2) Use HP-20 macroporous adsorption resin to separate PSPHs. After loading the sample, use distilled water and gradient elution with volume fractions of 29%, 49%, 69% and 90% ethanol solution, and collect 49% ethanol solution for elution The components were freeze-dried to obtain peptide powder I;

[0040] (3) Separate the peptide powder I with a Sephadex G-25 Sephadex chromatographic column, elute with distilled water as the mobile phase, obtain three components in sequence according to the elution time, and collect the last eluted component , ...

Embodiment 3

[0044] (1) Remove the head and viscera of the saury, wash the blood and twist it into surimi, mix the surimi and water at a mass ratio of 1:4, add a mixed enzyme of flavor protease and pancreatin, and add an amount of 1000U / g protein , under the conditions of pH 7.5 and temperature 50°C for 12 hours; at 95°C for 15 minutes, centrifuged for 20 minutes under a centrifugal force of 2500g, and the supernatant was taken to obtain saury protein enzymatic hydrolyzate (PSPHs), the degree of hydrolysis was 18.72% ±0.95%;

[0045] (2) Use HP-20 macroporous adsorption resin to separate PSPHs. After loading the sample, use distilled water and gradient elution with volume fractions of 20%, 40%, 60% and 80% ethanol solution successively, and collect 40% ethanol solution for elution The components were freeze-dried to obtain peptide powder I;

[0046] (3) Separate the peptide powder I with a Sephadex G-25 Sephadex chromatographic column, elute with distilled water as the mobile phase, obtai...

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Abstract

The invention belongs to the field of food biological techniques, and discloses a saury antioxidative peptide as well as a separation and extraction method and application thereof. The saury antioxidative peptide has an amino acid sequence as shown in Ser-Gly-Ala-Ala-Met. The separation and extraction method comprises the following steps: performing enzymolysis on saury by using protease, thereby obtaining a saury proteolysis product; separating the saury proteolysis product by using macroporous adsorption resin, thereby obtaining peptide powder I; separating the peptide powder I by using a sephadex chromatographic column, thereby obtaining peptide powder II; further purifying the peptide powder II by using a high efficiency liquid chromatogram column, thereby obtaining saury antioxidative peptide. The saury antioxidative peptide disclosed by the invention is relatively high in antioxidation property, and can be used in fields such as food, cosmetics and medicines.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a saury antioxidant peptide and its separation and extraction method and application. Background technique [0002] Saury belongs to the pelagic fish, the price is low, and its meat is rich in protein. Saury protein and its enzymatic hydrolyzate not only contain essential amino acids required by the human body, but also contain a large number of amino acids with high nutritional value, such as His, Tyr and Met, and other amino acids with antioxidant activity. Therefore, saury protein can be used as Good source for preparing antioxidant active peptides. At present, the research on saury at home and abroad mainly focuses on storage and fishing, although a small number of researchers have studied its processing by-products in order to increase the added value of saury, and extracted active ingredients from different parts for use in saury. In the industry, but only fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/20A23L1/305A23L33/18
Inventor 赵强忠赵谋明苏国万刘丹
Owner SOUTH CHINA UNIV OF TECH
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