Method for co-producing high-purity heparin sodium and dermatan sulfate from pig lungs

A technology of dermatan sulfate and heparin sodium, applied in the biological field, can solve the problems of impurities, containing dermatan sulfate, etc., and achieve the effect of reducing process steps, shortening production cycle, and improving purity

Inactive Publication Date: 2015-04-22
CHONGQING DOUHAO BIOTECH
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Problems solved by technology

[0011] The task of the present invention is to provide a kind of method extracting dermatan sulfate from pig lung and a kind of method extracting heparin from pig lung, to improve the production technology of existing crude product heparin sodium, improve the purity of heparin sodium product, to solve The crude heparin currently produced contains a large amount of dermatan sulfate impurities, and it can be used to produce injection-grade high-quality heparin sodium and pharmaceutical grade dermatan sulfate

Method used

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Embodiment 1

[0032] The method steps of this embodiment are as follows:

[0033] (1) Take fresh pig lungs (or frozen pig lungs after natural thawing), clean and decontaminate, peel off the trachea, mince them into minced meat to obtain pig lung slurry, take 1000Kg pig lung slurry, add 1500 liters of deionized water , at 25°C, stirred and reacted for 2 hours to obtain porcine lung slurry;

[0034](2) Add 135Kg sodium chloride to the pig lung slurry obtained in the previous step, adjust the pH to 10 with sodium hydroxide solution, stir evenly, and stir once every 2 hours at 26°C, 10 minutes each time, and react for 7 hours , to obtain pig lung alkaline hydrolyzate;

[0035] (3) Slowly heat the pig lung alkaline hydrolyzate obtained in the previous step to 40°C, adjust the pH to 9, add 1500g of heparin sodium complex protease, stir well, keep warm for 1 hour, then slowly heat up to 55°C, keep warm for 2 hour, then heated up to 90°C, kept warm for 15 minutes, and filtered through a 100-mesh ...

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Abstract

The invention provides a method for co-producing high-purity heparin sodium and dermatan sulfate from pig lungs. According to the method, the pig lungs are adopted as a raw material for producing high-purity heparin sodium and high-purity dermatan sulfate through enzymolysis, ion exchange, classified elution, concentration and ethanol precipitation. Specifically, the method comprises the steps of obtaining two steps of eluate through substep elution with ion exchange, removing salt and concentrating by using an ultrafiltration method, and further performing alcohol precipitation, thereby obtaining the heparin sodium and dermatan sulfate. In elution of ion exchange resin, a gradient sodium chloride solution is adopted for elution, so that heparin sodium and dermatan sulfate can be separated in eluate of different concentrations, the separation of heparin sodium from dermatan sulfate is achieved, the purity of heparin sodium and dermatan sulfate is respectively improved, the titer of heparin sodium and dermatan sulfate is also improved, and the purity, the titer and the optical rotation of heparin sodium and dermatan sulfate all meet the requirements of a pharmacopeia. The method is simple in process, low in cost and high in yield, and the economic benefits of product can be greatly increased.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a preparation method of heparin sodium and dermatan sulfate, in particular to a method for co-producing heparin sodium and dermatan sulfate from pig lungs. Background technique [0002] Heparin sodium is a glycosaminoglycan with a high content of N-sulfuric acid and iduronic acid. Clinically, it is mainly used for vascular embolism diseases, cardiovascular surgery, cardiac catheterization, extracorporeal circulation and hemodialysis. [0003] Heparin sodium widely exists in mammalian liver, lung, and intestinal mucosa, and mostly exists in complexes combined with proteins. Enzymatically hydrolyzed protein can separate heparin sodium, which is negatively charged at pH8-9, and can perform ion exchange with anion exchanger for rough separation. The crude fraction was precipitated in high-concentration ethanol for refinement and purification. [0004] For a long time, the production of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10C08B37/08
Inventor 邓小玲宁发子
Owner CHONGQING DOUHAO BIOTECH
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