A kind of preparation method and application of magnetic beads

A technology of magnetic beads and magnetic nanoparticles, which is applied in the manufacture of inductors/transformers/magnets, magnets, and magnetic objects. Simple method, small size effect

Active Publication Date: 2019-05-03
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above two types of magnetic beads all immobilize magnetic nanoparticles by coating (including physical coating and coating based on chemical bond immobilization) in the process of preparing non-magnetic microspheres, which inevitably increases the concentration of non-magnetic substances. Content, thereby reducing the magnetic content of the prepared magnetic beads, this is an inherent inherent contradiction, it is difficult to avoid from the method

Method used

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  • A kind of preparation method and application of magnetic beads
  • A kind of preparation method and application of magnetic beads
  • A kind of preparation method and application of magnetic beads

Examples

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Effect test

Embodiment 1

[0032] This embodiment provides a method for preparing magnetic beads, comprising the following steps:

[0033] (1) Preparation of magnetic nanoparticles modified by citric acid: the prepared Fe 3 o 4 The water-based dispersion of magnetic nanoparticles was ultrasonically dispersed for 15 minutes, and 20 mL was placed in a three-neck bottle for deoxygenation with nitrogen for 20 minutes, and then added to a certain amount of sodium citrate solution for 15 minutes to make Fe 3 o 4 The magnetic nanoparticles are evenly dispersed in the system, connect the experimental device, maintain the nitrogen environment, adjust the speed of mechanical stirring to 600rpb, raise the temperature of the water bath to 60°C, stir and react for 1h, use NdFeB magnets for magnetic separation, triple distilled water Wash to remove excess sodium citrate until magnetic separation can not obtain a clear supernatant, and finally re-disperse in 20mL triple distilled water to obtain citric acid-modified w...

Embodiment 2

[0046] The preparation method of a magnetic bead provided in this embodiment is basically the same as the preparation method of the magnetic bead described in Example 1, the difference is that:

[0047] (1) Preparation of surface-modified magnetic nanoparticles with small molecular functional groups: Small functional groups (including hydroxyl, amino, aldehyde, mercapto and carboxyl contained in other carboxylic acids different from citric acid) Molecular functionalization reagents, instead of citric acid in step (1) in Example 1, are used for similar surface modification of magnetic nanoparticles to obtain surface-modified magnetic nanoparticles with different functional groups.

[0048] (2) Preparation of magnetic nanoparticles with surface-immobilized macromolecules: macromolecules that need to be surface-immobilized, including polymer macromolecules, block polymers connected by chemical bonds, proteins, DNA, polypeptides, polysaccharides, etc., will replace this implementat...

Embodiment 3

[0051] The preparation method of a magnetic bead provided in this embodiment is basically the same as the preparation method of the magnetic bead described in Example 1, the difference is that:

[0052] (1) Preparation of magnetic nanoparticles embedded in PEI: Take 80mL of three-distilled water in a 250mL four-neck bottle, pass nitrogen gas to remove oxygen for 20min, connect the experimental device, check the airtightness to ensure good airtightness; Adjust the temperature to 35°C, adjust the speed of mechanical stirring to 300rpb, maintain the nitrogen environment in the four-necked bottle, and accurately weigh 4.05g of FeCl 3 ·6H 2 O and 1.98 g FeCl 2 4H 2 0, join in the four-neck bottle and stir and dissolve for 20min, add a certain amount of polymer macromolecular PEI (Mw=10000) in the ammonia solution of a certain concentration, adjust the rotating speed of the mechanical stirring to 600rpb, slowly add the ammonia diluent dropwise until The pH of the solution was 11,...

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Abstract

The invention discloses a magnetic bead preparing method and application, and belongs to the technical field of magnetic material preparing. The preparing method comprises the steps that firstly, magnetic nanoparticles with the surface functionalization are prepared, then, at least two magnetic nanoparticles with the surface functionalization are connected through a cross-linking agent, and a magnetic bead with the magnetic content of 70 %-98 % is prepared. The problems that the magnetic content of an existing magnetic bead is low, to achieve fast separation, the size of the magnetic bead is greatly increased, and consequently the suspension stability is reduced are solved. In addition, for the captured component biological reaction, particularly for the immunoreaction, the steric hindrance caused by the small magnetic bead is obviously reduced, the capturing capability and the analyzing and detecting sensitivity are improved, the magnetic bead can be better applied to the biological medicine separation and analysis, and the magnetic bead is particularly suitable for separation of cells, bacteria, viruses, DNA / RNA, protein and the like in biological body fluid or reaction fluid and the clinical biochemistry fast automatic detection based on chemiluminiscence electrochemical luminescence and fluorescence.

Description

technical field [0001] The invention belongs to the technical field of magnetic material preparation, and in particular relates to a preparation method and application of magnetic beads. Background technique [0002] Magnetic nanoparticles synthesized by conventional methods usually exhibit the following two remarkable features. First, it exhibits superparamagnetism or near superparamagnetism (little or very little remanence), which is conducive to the accumulation and separation from the dispersed solution under the action of a magnetic field, and is also conducive to redispersing into the solution after canceling the magnetic field effect. In the process of realizing the analysis of the separated components, the above-mentioned features are particularly beneficial to automatic separation and analysis; second, the particle size (referring to the non-hydrated particle size characterized by TEM) is small, usually 10-100nm, and each particle is in the Under the action of a st...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01F41/02H01F7/00G01N33/50
Inventor 谢洪平曾启胡紫阳
Owner SUZHOU UNIV
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