Special seafood mushroom liquefaction spawn culture medium and corresponding culture method thereof

Abstract

The invention discloses a special seafood mushroom liquefaction spawn culture medium. The special spawn culture medium is composed of ingredients based on the following parts by weight: 50-60 parts of corn starch, 1-2 parts of glucose, 10-15 parts of wheat cellulose, 15-25 parts of fine bran, 1-1.5 parts of yeast extract, 1-1.5 parts of beef peptone, 2-3 parts of mono-potassium phosphate, 1-1.5 parts of magnesium sulfate, and 100 parts of water. The invention further discloses a seafood mushroom liquefaction spawn culture method based the culture medium, and the culture method comprises the following steps of: disinfecting the special seafood mushroom liquefaction spawn culture medium under high temperature and pressure, accessing seafood mushroom mother seeds in the disinfected culture medium under a sterile condition, and culturing for 20-22 days at 20-22 degrees centigrade in the dark; the special prepared liquefaction solid spawn is diluted to prepare the seafood mushroom liquefaction spawn.

Description

technical field [0001] The invention relates to a special strain formula and cultivation method for liquefaction of seafood mushrooms. Background technique [0002] Edible fungus is one of the advantageous industries with the most modern agricultural development characteristics in China. This is not only because China is the world's largest producer and consumer of edible fungi, with a wide range of edible fungus production, but more importantly, the edible fungus industry is a good carrier and prominent position in the industrialization of biotechnology and the sustainable development of agriculture. At present, my country's edible fungus industry system has many factors that are not suitable for the global market economy, the most important of which are the intensification of industrial operations, low degree of specialization, backward production factors, imperfect production technology, and lack of key technology support. The most representative one is the intensive and ...

AI Technical Summary

Problems solved by technology

Remarks: The liquid fermentation strain process theoretically has a high purity of the strain, and the bacterial contamination rate of large-scale fermentation production is between 1% and 5% (in a 3-stage fermenter), and the bacterial contamination (mainly bacterial) is often In the later stage of fermentation, it is difficult to find out by conventional on-line inspection, which leads to production accidents caused by the inoculation of liquid fermentation strains and the contamination of large quantities of mushroom bags, which is a painful lesson.

In addition, because the cultivation of liquid strains requires nutrients such as high-concentration organic nitrogen and sugar sources, these ingredients remain in the bacterial liquid and are inserted into the fruiting bag. Since bacteria are inevitably brought in during operation, the residual nutrients It becomes a breeding ground for miscellaneous bacteria, which increases the risk of contamination after inoculation of the fruiting bag

This is the main problem that liquid fermentation strains cannot be applied to the production of edible fungi on a large scale

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