Nitrobacter winogradsky XY-01, as well as preparation method and application thereof

A technology of Nitrobacter victoria and its cultivation method, which is applied in the field of Nitrobacter victoria XY-01 and its cultivation, can solve the problems of easy rebound of nitrite in water body, large amount of adsorbent, short maintenance time, etc., and achieve fast degradation rate , long-lasting effect, high concentration effect

Active Publication Date: 2015-04-29
TIANJIN CARING TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the traditional oxidation or reduction method can safely and effectively reduce the concentration of nitrite, it has the disadvantages of short maintenance time and easy rebound of nitrite in water; although the physical ...

Method used

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  • Nitrobacter winogradsky XY-01, as well as preparation method and application thereof
  • Nitrobacter winogradsky XY-01, as well as preparation method and application thereof
  • Nitrobacter winogradsky XY-01, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Screening and identification of Nitrobacter victoria XY-01

[0065] Take 1g of aquaculture pond sediment sample and dissolve it in 100mL enrichment and separation medium prepared, cultivate it in a 30°C incubator at 180r / min until no nitrite nitrogen can be detected, then absorb 5mL of the culture product to transfer Continue culturing in a new medium, repeat 3 times, and finally dilute the culture product according to gradient, 10 -1 、10 -2 …until diluted to 10 -7 , each concentration was spread on the plate, and a single colony was picked and streaked and purified 2-3 times repeatedly, and the purified strain was placed on the slant, and no heterotrophic bacteria grew through the beef extract-peptone medium and LB medium; the colony was in The culture plate was in the shape of transparent round fine particles, and Gram staining was negative. After 16SrDNA sequencing and identification, it was determined to be Nitrobacter victoria. The sequence of the 16SrD...

Embodiment 2

[0068] Example 2 Degradation of nitrite by Nitrobacter victoria XY-01

[0069] The specific implementation steps are as follows: the bacterial strain is inoculated into the fermentation medium 1, cultured with shaking at 30°C, and the concentration of nitrite nitrogen in the culture solution is measured according to the "Spectrophotometric Method for Determination of Nitrite Nitrogen in Water Quality (GB7493-87)". When the nitrate nitrogen concentration is lower than 30mg / L, add 40% sodium nitrite solution until the nitrite nitrogen concentration in the culture medium is about 200-400mg / L, until the bacterial concentration is OD 600 When it reached 0.3 or higher, the culture was terminated, the bacteria were collected by centrifugation at 9000r / min, all were transferred to the same amount of fermentation medium 2 (the concentration of nitrite nitrogen was about 240mg / L), and cultured on a shaking table at 30°C and 150r / min. Sample 5mL every 1h, centrifuge at 9000r / min to get t...

Embodiment 3

[0070] Example 3 Degradation of nitrite by Nitrobacter victoria XY-01

[0071] The specific implementation steps are as follows: the bacterial strain is inoculated into the fermentation medium 2, cultured with shaking at 30°C, and the concentration of nitrite nitrogen in the culture solution is measured according to the "Spectrophotometric Method for Determination of Nitrite Nitrogen in Water Quality (GB7493-87)". When the nitrate nitrogen concentration is lower than 30mg / L, add 40% sodium nitrite solution until the nitrite nitrogen concentration in the culture medium is about 200-400mg / L, until the bacterial concentration is OD 600 When it reaches 0.3 or more, the culture is ended, and it is transferred to the fermenter to expand the culture until the bacterial concentration is OD 600 When it reaches above 0.3, the bacterial concentration increases to OD after concentrated treatment by a tubular centrifuge 600 ≈3.0, inoculate 0.5mL of the bacterial solution into 200mL of fer...

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Abstract

The invention provides nitrobacter winogradsky XY-01 with the preservation number of CGMCC (China General Microbiological Culture Collection Center ) 10171, the nitrobacter winogradsky XY-01 can be subjected to fermentation cultivation by adopting a batch feeding way, a cultivated product can be used for removing nitrite nitrogen in aquatic water, the problem of nitrite accumulation in the aquatic water can be effectively solved, the fish and shrimp yield is increased, and the nitrobacter winogradsky XY-01 has a wide application prospect in the field of aquaculture.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to Nitrobacter winogradskyi XY-01 and its cultivation method and application. Background technique [0002] Nitrite is a salt of nitrous acid, containing nitrite ion NO 2 - , toxic, carcinogenic, and generally soluble in water except silver nitrate. Alkali metal salts are relatively stable, and other salts decompose when heated. Both oxidizing and reducing. It is widely used in chemical synthesis, food industry and as a necessary chemical reagent in the laboratory. [0003] In aquaculture, fish and shrimp residual bait and their excrement decompose in water to produce a large amount of ammonia and toxic substances, which are quickly converted into nitrous acid through the action of nitrosative bacteria and photosynthetic bacteria, and nitrous acid and metal ions in the water body The combination will produce nitrite. In addition, if the silt at the bottom of the pond is not cleared...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/16C12N1/20C12N1/205C12R2001/01
Inventor 李玉庆张建孙晓莹薛二军
Owner TIANJIN CARING TECH DEV
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