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A pretreatment process of sugarcane molasses and a process for producing DHA by fermentation

A sugarcane molasses, pre-treatment technology, applied in the directions of fermentation, microorganisms, microorganism-based methods, etc., can solve the problems of cytotoxicity, affecting the yield and quality of fermented products, increasing the salinity of fermentation liquid, etc., and achieving the effect of low price

Inactive Publication Date: 2018-08-03
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that it contains a large amount of colloid and ash, which affects the yield and quality of fermented products to varying degrees: the colloidal substance makes the molasses more viscous, resulting in poor fluidity of the fermentation broth, resulting in difficulties in the conduction of oxygen, heat and nutrients; The ash content is as high as 10-15%, resulting in an increase in the salinity of the fermentation broth and toxicity to cell growth, so the molasses must be pretreated to remove colloids, ash and other impurities in a targeted manner

Method used

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  • A pretreatment process of sugarcane molasses and a process for producing DHA by fermentation
  • A pretreatment process of sugarcane molasses and a process for producing DHA by fermentation
  • A pretreatment process of sugarcane molasses and a process for producing DHA by fermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1) Pre-treatment of sugarcane molasses:

[0039] Using molasses from Daxin Feilong Sugar Factory in Guangxi as the raw material for fermentation experiments, dilute the molasses with distilled water to a Brix of 50 o BX, add concentrated sulfuric acid to adjust the pH to 2, heat in a water bath at 70°C for 60 minutes, fully hydrolyze the sucrose into reducing sugar, add milk of lime to adjust the pH to 7.0 when the temperature of the sugar liquid drops to 65°C, keep it warm for 30 minutes to flocculate impurities, and add polyacrylamide 3mg / L , keep warm for 10min for secondary flocculation, centrifuge at 8000r / min for 10min, and take the supernatant for later use. The sucrose in the treated supernatant is basically decomposed into reducing sugars, most of the colloids in the molasses are removed, the removal rate of potassium ions in the molasses is 50%, and the content of calcium ions is slightly reduced.

[0040] 2) Preparation of medium:

[0041] Common seed cultu...

Embodiment 2

[0052] 1) Pre-treatment of sugarcane molasses:

[0053] Using molasses from Daxin Feilong Sugar Factory in Guangxi as the raw material for fermentation experiments, dilute the molasses with distilled water to a Brix of 65 o BX, add concentrated sulfuric acid to adjust the pH to 4, heat in a water bath at 95°C for 50 minutes to fully hydrolyze the sucrose into reducing sugar, add milk of lime to adjust the pH to 7.0 when the temperature of the sugar solution drops to 75°C, keep it warm for 30 minutes to flocculate impurities, add polyacrylamide 3mg / L, Incubate for 10 minutes for secondary flocculation, centrifuge at 8000r / min for 10 minutes, and take the supernatant for later use. Sucrose in the treated supernatant is basically decomposed into reducing sugar, wherein the proportion of sucrose is 3.3%, and the proportion of reducing sugar is 46.7%. Most of the colloids in the molasses have been removed, and the removal rate of potassium ions in the molasses is 53%. Slightly red...

Embodiment 3

[0066] 1) Pre-treatment of sugarcane molasses:

[0067] The molasses of Guangdong Wengyuan Maoyuan Sugar Factory was used as the raw material for the fermentation experiment, and the molasses was diluted with distilled water to a Brix of 55 o BX, add concentrated sulfuric acid to adjust the pH to 2, heat in a water bath at 95°C for 30 minutes to fully hydrolyze the sucrose into reducing sugar, add milk of lime to adjust the pH to 7.0 when the temperature of the sugar solution drops to 75°C, keep it warm for 30 minutes to flocculate impurities, add polyacrylamide 3mg / L, Incubate for 10 minutes for secondary flocculation, centrifuge at 8000r / min for 10 minutes, and take the supernatant for later use.

[0068] The results are shown in Table 4:

[0069]

[0070]

[0071] The results in Table 4 show that the sucrose in the treated supernatant is basically decomposed into reducing sugars, and most of the colloids in the molasses are removed. The potassium ion removal rate in ...

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Abstract

The invention discloses a cane molasses pretreatment process and fermentation and production process. After a series of treatments of acidification, preliming, flocculation and the like are conducted on sugar industry by-product cane molasses, flocculate impurity is removed, and a molasses treating liquid conforming to DHA fermentation and production by schizochytrium limacinum can be obtained. Through the treatment, the cane sugar in the cane molasses is mainly resolved into reducing sugar, most colloid is removed, the removal rate of potassium ion is not less than 50%, the content of calcium ion is slightly reduced, and the fermentation and production requirements of schizochytrium limacinum are met. The pretreated cane molasses is utilized to be added into a schizochytrium limacinum culture medium according to the ratio of being 15-60% of that of the total reducing sugar to ferment and produce DHA. The added cane molasses treating liquid not only can provide part carbon source such as glucose and levulose for growth of schizochytrium limacinum, but also contains necessary vitamin and mineral element needed by growth and grease synthesis, is relatively low in price, and can be used as an excellent cheap raw material for DHA fermentation and production.

Description

technical field [0001] The invention relates to the fermentative production of DHA by using Schizochytrium fungus, in particular to the pretreatment process and the fermentative production process of sugarcane molasses as a carbon source. Background technique [0002] Docosahexaenoic acid (DHA) is an important omega-3 series long-chain unsaturated fatty acid, which is one of the essential fatty acids in the human body. It has the functions of promoting brain and retinal development, anticoagulation, lowering blood fat, preventing Important effects such as Alzheimer's disease and cancer. The human body can only use the linolenic acid obtained from food to synthesize a small amount of DHA, which cannot meet the nutritional needs of the body. Therefore, it has become a consensus among people to obtain DHA from diet. [0003] At present, the main source of DHA is marine fish oil. Fish oil products have disadvantages such as low DHA content, complex fatty acid composition, diffi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427
Inventor 陈骏佳班甲李浩洋徐晓燕付尽国王帆胡雪梅班雯婷张卫东田鑫
Owner GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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