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Preparation method of anti-hepatoma Chinese medicinal effective part composition

A technology of effective parts and compositions, which is applied in the field of preparation of effective part compositions of traditional Chinese medicine, and can solve problems such as dark color, hidden onset of liver cancer, and many impurities

Active Publication Date: 2015-05-06
广州泽力医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Liver cancer has the characteristics of insidious onset, rapid progression, poor prognosis, and short survival period. Statistics show that the incidence of liver cancer accounts for 5% of all tumor diseases. There are about 260,000 new cases worldwide every year, but the 5-year survival rate less than 5%
However, the traditional separation and purification process of traditional Chinese medicine products has the disadvantages of many impurities, large dosage, dark color, etc., which seriously hinders the application and promotion of traditional Chinese medicine products.

Method used

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  • Preparation method of anti-hepatoma Chinese medicinal effective part composition
  • Preparation method of anti-hepatoma Chinese medicinal effective part composition
  • Preparation method of anti-hepatoma Chinese medicinal effective part composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Raw materials (percentage by weight):

[0028] Phyllostachys 5%, Sophora flavescens 18%, Xihuangcao 20%, Panax notoginseng 10%, Salvia miltiorrhiza 25%, Peach kernel 4%, Bupleurum 4%, Ligustrum lucidum 5%, Lilycystis 5%, Pseudostellaria 2 %, licorice 2%;

[0029] Major equipment:

[0030] Crusher, differential pressure extractor, inorganic membrane filter, reverse osmosis concentrator, thin film evaporation concentrator, vacuum dryer.

[0031] Preparation process:

[0032] 1. Medicinal material pretreatment: each single medicinal material is crushed into 20 mesh medicinal material powder and mixed evenly.

[0033] 2. Extraction: Take 65 kg of 50% ethanol and mix it evenly with the compound medicinal material powder, extract through continuous pressure difference for 30 minutes, centrifuge to obtain centrifuged residue and centrifuged liquid, add 60 kg of 50% ethanol to the centrifuged residue, stir and extract for 30 minutes, centrifuge and clean the centrifuge The ...

Embodiment 2

[0037] Embodiment 2: the active experiment of embodiment 1

[0038] In this example, experiments have proved that No. 1, No. 2 and No. 3 compositions of active parts of anti-liver cancer traditional Chinese medicines have good anti-liver cancer effects, and No. 2 and No. 3 have better effects.

[0039] 1 Materials and methods

[0040] 1.1 Test drugs

[0041] Name: No. 1, No. 2 and No. 3 compositions of effective parts of anti-liver cancer traditional Chinese medicine.

[0042] 1.2 Cell lines:

[0043] HSC-T6 cell line.

[0044] 1.3 Main reagents

[0045] Sugar DMEM culture medium and fetal bovine serum were purchased from Gibco Company of the United States; MTT, DMSO and trypsin were purchased from Sigma Company of the United States.

[0046] 1.4 Experimental method

[0047] 1.4.1 Cell culture

[0048] The HSC-T6 cell line was resuscitated and inoculated in fetal bovine serum containing 10% volume fraction. Each of penicillin and streptomycin was 100U / mL, and the conce...

Embodiment 3

[0059] The raw material of embodiment 1 is changed into (percentage by weight):

[0060] Phyllostachys 20%, Sophora flavescens 10%, Xihuangcao 10%, Panax notoginseng 5%, Salvia miltiorrhiza 10%, Peach kernel 5%, Bupleurum 10%, Ligustrum lucidum 5%, Lilycystis 5%, Pseudostellaria 10 %, licorice 10%;

[0061] Others are the same as in Example 1, and No. 1, No. 2 and No. 3 anti-liver cancer traditional Chinese medicine effective fraction compositions are prepared. Embodiment 4: the active experiment of embodiment 3

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Abstract

The invention discloses a preparation method of an anti-hepatoma Chinese medicinal effective part composition. The method comprises the following steps: (1) preparing raw medicines which comprise the following components in percentage by weight: 5-30 percent of underleaf pearls, 5-20 percent of kuh-seng, 5-30 percent of serrate rabdosia herbs, 4-10 percent of pseudo-ginseng, 5-25 percent of danshen, 4-15 percent of peach kernels, 4-10 percent of bupleurum chinense, 5-25 percent of glossy privet fruits, 5-25 percent of yerbadetajo herbs, 2-25 percent of radix pseudostellariae and 2-10 percent of licorice roots; and (2) performing pressure difference type extraction and multistage membrane separation condensation combined extraction, and finally refining by using a column separation method. The anti-hepatoma Chinese medicinal effective part composition is prepared by utilizing the pressure difference type extraction and multistage membrane separation condensation combined extraction, and the effective part is refined by utilizing an adsorption resin column separation method, so that a large parts of impurities are removed, the oral administration quantity of patients can be reduced based on the guaranty of curative effects, and a new conception is provided for development and production of the traditional Chinese medicine. The effective part can clear away the heat and detoxify, activate the blood and remove stasis, sooth liver-qi and remove stagnation, tonify the spleen and the kidney, and inhibit the growth of tumor.

Description

technical field [0001] The invention relates to a preparation method of a traditional Chinese medicine effective part composition for anti-liver cancer. Background technique [0002] Liver cancer has the characteristics of insidious onset, rapid progression, poor prognosis, and short survival period. Statistics show that the incidence of liver cancer accounts for 5% of all tumor diseases. There are about 260,000 new cases worldwide every year, but the 5-year survival rate But less than 5%. As one of the countries with high incidence of liver cancer in China, the mortality rate of liver cancer has even been on the rise in the past 20 years, which seriously threatens the health of our people. [0003] At present, in addition to surgical resection, Western medicine mainly uses interventional therapy and chemotherapy in the treatment of liver cancer. However, even with radical resection, there is still a recurrence rate of 60% to 70% within 5 years; 30% of liver cancer patient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/736A61P1/16A61P35/00
Inventor 刘乡乡宋力飞彭宪周绍春
Owner 广州泽力医药科技有限公司
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