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Preparation method of a mesoporous apatite nano drug carrier with pH responsiveness and cell targeting to liver cancer cells

A nano-drug carrier and mesoporous apatite technology, applied in the field of biomedical materials, can solve the problems of non-specific recognition of target cells, increased drug toxicity and side effects, and low drug delivery ability, so as to reduce drug intake and cost Inexpensive, practical effect

Active Publication Date: 2017-10-03
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem that the carrier prepared by the existing method cannot specifically recognize the target cells, and most of the drug molecules are distributed in normal tissues and taken up by normal cells, while the drug distribution in diseased tissues is less, resulting in low drug delivery ability. Increased the technical problem of toxic and side effects of drugs in the body, thus providing a method for preparing a mesoporous apatite nano-drug carrier that is pH-responsive and cell-targeted to liver cancer cells

Method used

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  • Preparation method of a mesoporous apatite nano drug carrier with pH responsiveness and cell targeting to liver cancer cells
  • Preparation method of a mesoporous apatite nano drug carrier with pH responsiveness and cell targeting to liver cancer cells
  • Preparation method of a mesoporous apatite nano drug carrier with pH responsiveness and cell targeting to liver cancer cells

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specific Embodiment approach 1

[0033] Embodiment 1: A method for preparing a mesoporous apatite nano-drug carrier that is pH-responsive and cell-targeted to liver cancer cells in this embodiment is carried out in the following steps:

[0034] 1. Preparation of the template agent: Dissolve the template agent F127 and calcium pantothenate monohydrate in deionized water, and stir at a stirring speed of 250r / min to 400r / min until a clear emulsion is obtained to obtain the F127-calcium pantothenate mixed solution. Promptly obtain templating agent;

[0035] The mass ratio of template agent F127 described in step 1 to calcium pantothenate monohydrate is 1: (2.5-8); the mass ratio of F127 described in step 1 to deionized water is 1: (15-67);

[0036] 2. Preparation of mesoporous hydroxyapatite nanoparticles: K 2 HPO 4 ·3H 2 O is dissolved in deionized water to give K 2 HPO 4 solution, followed by NH 3 ·H 2 O Mediation K 2 HPO 4 The pH value of the solution is adjusted to a pH value of 10-14, and then the p...

specific Embodiment approach 2

[0055] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mass ratio of template agent F127 and calcium pantothenate monohydrate described in step 1 is 2.0:10.12. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0056] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the volume ratio of the template agent F127 described in step 1 to deionized water is 2.0 g: 80 mL. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

A method for preparing a mesoporous apatite nano drug carrier with pH responsiveness and cell targeting to liver cancer cells, the invention belongs to the field of biomedical materials. The present invention aims to solve the problem that the carrier prepared by the existing method cannot specifically recognize the target cells, and most of the drug molecules are distributed in normal tissues and taken up by normal cells, while the drug distribution in diseased tissues is less, resulting in low drug delivery ability. This increases the technical problem of toxic side effects of the drug in the body. Methods: 1. Preparation of template agent; 2. Preparation of mesoporous hydroxyapatite nanoparticles; 3. Amination of mesoporous hydroxyapatite; 4. Boration of mesoporous apatite; 5. Function of lactobionic acid Preparation of bovine serum albumin; 6. Preparation of mesoporous apatite nano drug carrier with pH responsiveness and cell targeting. The product obtained by the invention can target and recognize liver cancer cells, reduce the uptake of drugs by normal tissues, and reduce the toxic and side effects of drugs in vivo.

Description

Technical field: [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method of a mesoporous apatite nano drug carrier with pH responsiveness and cell targeting. Background technique: [0002] With the increasing incidence of malignant tumors year by year, the intelligent responsive drug delivery system has attracted more and more attention. The intelligent responsive system is that after arriving at the target affected area, the drug carrier generates an intelligent response to changes in the surrounding environment or external stimuli to achieve the purpose of controlled drug release. So far, a series of responsive release drug carriers have been constructed, such as temperature response, light response, and ultrasonic response. This type of system can exist stably in the circulation in the body. However, these responsive releases of drugs come from external additional stimuli rather than changes in the physiologica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/54A61K47/64A61K47/69A61K9/19A61K31/704A61K49/00A61P35/00A61K47/42A61K47/52
Inventor 黄玉东李大龙贺金梅李纪伟黄鑫程玮璐吴亚东
Owner HARBIN INST OF TECH
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