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A method for preparing clam protein-derived ACE inhibitory peptides using endogenous enzymes

A technology of blue clam protein and endogenous enzymes, which is applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of low processing and utilization, waste of high-quality protein resources, etc., and achieves low cost, easy control, and convenient operation. Effect

Inactive Publication Date: 2018-02-02
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the deficiencies of the prior art, the present invention provides a method of using endogenous enzymes to prepare ACE inhibitory peptides derived from clam protein, so as to solve the problem of waste of high-quality protein resources due to low processing and utilization of clam protein

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Fresh blue clams are shelled, washed and homogenized, and then placed in an enzymatic hydrolysis bottle; irradiated with ultraviolet light at 30W for 10 minutes; the pH of the blue clam homogenate is adjusted to 4.0 with 0.1 mol / L hydrochloric acid solution, and the temperature of the blue clam homogenate is controlled to 80°C, use the endogenous protease of blue clam to carry out enzymatic hydrolysis for 10 hours. During the enzymatic hydrolysis process, 0.1 mol / L sodium hydroxide is continuously added to maintain a constant pH; The activated carbon that accounts for 5% of the weight of the enzymolysis solution is used for deodorization, and the process of deodorization is continuously stirred to fully mix the activated carbon and the enzymolysis solution; the mixture of activated carbon and enzymolysis solution is separated by a separator; the obtained clarified solution is freeze-dried, The freeze-dried powder is the clam protein source ACE inhibitory peptide powder, ...

Embodiment 2

[0018] Fresh blue clams are shelled, washed and homogenized, and then placed in an enzymatic hydrolysis bottle; irradiated with ultraviolet light at 30W for 60 minutes; the pH of the blue clam homogenate is adjusted to 11.0 with 0.1 mol / L sodium hydroxide solution to control the blue clam homogenate When the temperature reaches 40°C, use the endogenous protease of the blue clam to carry out enzymatic hydrolysis for 1 hour. During the enzymatic hydrolysis, 0.1 mol / L sodium hydroxide is continuously added to maintain a constant pH; Add activated carbon accounting for 5% of the weight of the enzymolysis solution for deodorization, and stir continuously during the deodorization process to fully mix the activated carbon and the enzymolysis solution; the mixture of activated carbon and enzymolysis solution is separated by a separator; the obtained clarified solution is frozen The dried and freeze-dried powder is the clam protein source ACE inhibitory peptide powder, and the ACE inhib...

Embodiment 3

[0020] Fresh blue clams are shelled, washed and homogenized, and then placed in an enzymatic hydrolysis bottle; irradiated with ultraviolet light at 30W for 30 minutes; the pH of the blue clam homogenate is adjusted to 8.0 with 0.1 mol / L sodium hydroxide solution to control the blue clam homogenate When the temperature reaches 60°C, use the endogenous protease of the blue clam to carry out enzymatic hydrolysis for 5 hours. During the enzymatic hydrolysis, 0.1 mol / L sodium hydroxide is continuously added to maintain a constant pH; Add activated carbon accounting for 5% of the weight of the enzymolysis solution for deodorization, and stir continuously during the deodorization process to fully mix the activated carbon and the enzymolysis solution; the mixture of activated carbon and enzymolysis solution is separated by a separator; the obtained clarified solution is frozen The dry and freeze-dried powder is the clam protein source ACE inhibitory peptide powder, and the ACE inhibit...

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Abstract

The invention belongs to the field of deep processing of aquatic products and biotechnology, and in particular relates to a method for preparing ACE inhibitory peptide derived from blue clam protein by using endogenous enzymes. The present invention uses the endogenous protease in the clam for enzymolysis, and obtains the natural ACE inhibitory peptide through autolysis, without adding any exogenous protease in the preparation process, and the whole preparation process includes fresh clam pretreatment, homogenization, endogenous enzyme Activation, enzymatic hydrolysis, enzyme inactivation, deodorization, centrifugation, freeze-drying. The invention not only solves the important bottleneck problem existing in the basic research that restricts the extension and upgrading of the shellfish industry chain, but also opens up new high-quality protein resources.

Description

technical field [0001] The invention belongs to the field of deep processing of aquatic products and biotechnology, and in particular relates to a method for preparing ACE inhibitory peptide derived from blue clam protein by using endogenous enzymes. Background technique [0002] Blue clam (Aloididae aloidis) is an important low-value marine shellfish that is abundant in the coast of my country. The meat is delicious, and the shell is thin and fleshy. It is fully utilized and mostly used as live bait for prawns. Blue clams are rich in a variety of proteins, and the protein content is as high as 60%. The amino acid composition is comprehensive. Realize the utilization of blue clam protein resources. [0003] Active peptides have a variety of biological activities, are easily absorbed by the body, and have the convenience of chemical synthesis and chemical modification, attracting more and more research interests of researchers in food science, biology, chemistry and their in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14
Inventor 于志鹏赵文竹密更励建荣
Owner BOHAI UNIV
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