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Acidophilus sulfate reducing bacterium strain and application thereof

A sulfate and bacterial strain technology, applied in the field of microbial screening and application, can solve the problems of unknown bacterial structure, bacterial strains not resistant to acid, high cost, etc., and achieve the effect of overcoming uncertainty, good effect and vigorous metabolism

Active Publication Date: 2015-05-20
SHAOGUAN TAOLIN GREEN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the high cost of the prior art, the structure of the bacterial flora used is unknown, the strains are not acid-resistant, and the restoration effect is unstable. Control the disadvantages of mine soil acidification and provide a new strain of acidophilic sulfate-reducing bacteria

Method used

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  • Acidophilus sulfate reducing bacterium strain and application thereof
  • Acidophilus sulfate reducing bacterium strain and application thereof
  • Acidophilus sulfate reducing bacterium strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 FKB strain isolation identification and functional analysis

[0042] 1) Strain screening

[0043] The sediment samples collected from the edge of the No. 1 tailings pond of Fankou Lead-Zinc Mine were collected in a sterile 50mL centrifuge tube, then placed in an ice bath, transported back to the laboratory, and immediately stored in a 4°C refrigerator.

[0044] After the sample is collected, enrichment culture is first carried out with a special enrichment medium, the purpose of which is to allow the sulfate-reducing bacteria that do not occupy a dominant position to multiply in large numbers and remove most of the heterotrophic bacteria. The culture medium for aSRB enrichment refers to the culture medium used by Sen and Johnson (1999) for cultivating sulfate-reducing bacteria, and corresponding improvements are made according to the actual situation. The composition of enrichment medium is: (NH 4 ) 2 SO 4 0.45g; KCl0.05g; MgSO 4 ·7H 2 O 0.5g; KH 2 PO ...

Embodiment 2F

[0051] The preparation of embodiment 2FKB liquid bacterial agent

[0052] 1) Inoculate the FKB bacteria into the enrichment medium for cultivation. Use a 100mL blue-capped glass bottle. After inoculation, blow nitrogen for 20 minutes to remove the air, and then place it in a 30°C incubator for static cultivation until the culture medium changes from clarification to ink color;

[0053] 2) Inoculate the cultured bacterial solution into a 10L seed tank according to the inoculum amount of 5%, blow nitrogen gas for 20 minutes, and cultivate at 30°C until the culture solution changes from clarification to ink color, and the liquid volume in the seed fermentation tank is 70%. The medium formula (w / v) is: sugarcane molasses 5%, yeast extract 2%, glucose 0.5%, (NH 4 ) 2 SO 4 4.5%, MgSO 4 ·7H 2 O 5%, KCl 0.5%, KH 2 PO 4 0.5%, Ca(NO 3 ) 2 4H 2 O 0.14%, FeSO 4 ·7H 2 O5%, ascorbic acid 0.5%, pH 5.2;

[0054] 3) Inoculate the fermented liquid in the seed fermented tank into th...

Embodiment 3F

[0056] Example 3 FKB is used for acidification control of dump site

[0057] The site is the southern dump of Jiangxi Yongping Copper Mine, with an area of ​​16,800 square meters, divided into two areas: the top and the side slope, and the side slope is 10,000m 2 , top 6800m 2 . This area has the following typical representative meanings:

[0058] (1) The slope is steep and the drop is large: According to the surveyed topographic map, the elevation of the top of the area is 273.3m, the elevation of the foot of the slope is 185.5m, the elevation difference reaches 87.8m, and the slope of the slope is between 1:1 and 1:1.25. It can basically represent the terrain of the dump site with steep slope and large drop.

[0059] (2) Severe erosion and piles of boulders: Due to years of erosion and erosion, more than 60% of the area’s slopes and slope toes are basically boulders without soil, and the living conditions for plants are extremely poor (no plants were seen on site growth)...

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Abstract

The invention discloses an acidophilus sulfate reducing bacterium strain FKB and a method for acidification control with the strain. The acidophilus sulfate reducing bacterium strain FKB is desulfospornosinus sp, and is preserved at the China General Microbiological Culture Collection Center (CGMCC for short) on November 27, 2014; and the preservation number is CGMCC No.10072. The strain is prepared into a liquid microbial inoculum after fermentation culture; and soil is simply improved with lime, chicken manure, a phosphate fertilizer and the like and then the liquid microbial inoculum can be used together with a plant stabilizing technology to perform the acidification control on soil of mining wastelands such as refuse dumps and tailings ponds; and the targets of acidification restraint, ecological regreening and mine land rehabilitation are reached.

Description

technical field [0001] The invention belongs to the field of screening and application of microorganisms, and in particular relates to an acidophilic sulfate-reducing bacteria strain and a method for controlling acidification thereof. Background technique [0002] The problem of soil acidification in abandoned non-ferrous metal mines is widespread all over the world. As a traditional mining country, China currently has more than 100,000 mines. Uninterrupted mining activities have produced a huge area of ​​mining wasteland, and most of the mining wasteland has not been properly treated. The serious soil acidification problem has a negative impact on the entire ecology. Huge damage has been caused to the environment, no grass can grow in the acidified area, and the discharge of toxic heavy metal acid water is serious. The main reason for the acidification of these mining wastelands is that most non-ferrous metal ore formations have various types of metal sulfides (mainly FeS ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00A01B79/02A01G1/00C12R1/01
CPCA01B79/02C09K17/00C12N1/20C12N1/205C12R2001/01
Inventor 张金桃廖斌杨涛涛屈燕妮陈文册
Owner SHAOGUAN TAOLIN GREEN TECH
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