Large-scale manufacturing method for high-precision BL culture medium

Abstract

The invention discloses a large-scale manufacturing method for a high-precision BL culture medium, and belongs to the field of plant biotechnology. The large-scale manufacturing method comprises the following steps: firstly, calculating required amount, which is increased by 1000 times, of 16 elements contained in the medium as well as the required amount, which is increased by 10000 times, of CuSO4.5H2O and CoCl2.6H2O which have low requirements; accurately weighing the drugs; mixing the 16 elements according to the required amount, and sealing; dissolving CuSO4.5H2O and CoCl2.6H2O with 1L of distilled water for later use; respectively opening according to the needs for use. The substrate increased by 1000 times prepared by the large-scale manufacturing method disclosed by the invention has the characteristics of being high in precision, convenient and quick to operate, simple to apply, and small in errors of different batches and within the batches, phenomena of large errors, precipitation, mildewing and the like caused by manual operation and external factor interferences during the preparation of high-precision BL liquid culture medium are avoided. The large-scale manufacturing method can be applied to the biotechnical purposes of plant tissue culture and the like.

Description

technical field [0001] The invention discloses a large-scale production method of a high-precision BL culture substrate, which belongs to the field of plant biotechnology. More specifically, it includes six steps including accurate calculation, accurate weighing, mixing less and more, sealing, separate mother liquor preparation, unpacking and using, in order to obtain different batches and batches with minimal error A method for cultivating substrates and reducing experimental errors. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the ...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters