Double-tube vacuum film sperm separation method and separation device

Abstract

The invention discloses a double-tube vacuum film sperm separation method and a separation device and mainly relates to the field of cytobiology. The separation device comprises an inner centrifugal tube and a centrifugal tube, wherein the lower part of the inner centrifugal tube is of a cone structure which is opened at the tip; a filter device is mounted in the inner centrifugal tube; an inner tube seal cover is arranged at the orifice at the top of the inner centrifugal tube. By using the device to separate a seminal fluid, a density gradient solution is prepared by adopting a classical density gradient sperm separation method and a sperm lotion and sperms are sequentially dropped onto the filter device, and the inner centrifugal tube after low-speed centrifugal treatment is taken out, so that the inner centrifugal tube a is separated from the centrifugal tube, and the residues in the centrifugal tube are part of alpha% density gradient solution and bottom spermatia. The method and device disclosed by the invention have the beneficial effects that whether the seminal fluid is liquefied or not, seminal plasma and sperms are thoroughly separated through a simple operation; the damage on sperms in the pre-treatment process of the seminal fluid is avoided; the success rate, the recovery rate and the quality of sperms are excellent.

Description

technical field [0001] The invention relates to the field of cell biology, in particular to a double-tube vacuum membrane sperm separation method and a device. Background technique [0002] Sperm separation processing technology is an indispensable key technology in assisted reproductive technology. The commonly used sperm separation technology in the implementation of assisted reproductive technology mainly includes density gradient centrifugation technology and upstream separation method. Each of these techniques has its own limitations. The sperm recovery rate of the density gradient centrifugation technique is relatively high, but after the treatment is completed, the upper impurities and low-density solution need to be removed with a straw, and then the sperm at the bottom of the centrifuge tube is sucked through the liquid surface of the high-density solution with a straw. It adheres to the wall of the centrifuge tube and flows downstream to the liquid surface of the ...

AI Technical Summary

Problems solved by technology

In practice, we found that the liquefied semen can be obtained by passing the non-liquefied human semen through filter paper and centrifuging, but it is difficult for the semen to pass through the filter paper by its natural filtration, and the recovery rate is low, which affects the separation and treatment of semen

Images