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Method for detecting recovery rate of [2,2-bis(4-hydroxyphenol)propane] absorbed by using aptamer functional magnetic nanomaterial

A magnetic nano-functionalization technology, applied in the field of detection of organic matter, can solve the problems of complex pretreatment methods, BPA leaching, ingestion, etc., and achieve the effect of simple method, low detection limit and good selectivity

Inactive Publication Date: 2015-05-27
王利兵
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Problems solved by technology

[0005] (3) Repeated use of BPA-containing items or exposure to high-heat environments can also lead to BPA leaching, which can then be ingested by the human body
However, the commonly used preprocessing methods are relatively complex and time-consuming.

Method used

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  • Method for detecting recovery rate of [2,2-bis(4-hydroxyphenol)propane] absorbed by using aptamer functional magnetic nanomaterial
  • Method for detecting recovery rate of [2,2-bis(4-hydroxyphenol)propane] absorbed by using aptamer functional magnetic nanomaterial
  • Method for detecting recovery rate of [2,2-bis(4-hydroxyphenol)propane] absorbed by using aptamer functional magnetic nanomaterial

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Embodiment Construction

[0030] A method for magnetic separation of bisphenol A by aptamer functionalized magnetic nanomaterials, synthesis of magnetic nanoparticles, aptamer functionalized magnetic nanoparticles, and aptamer functionalized magnetic biosensors to adsorb target bisphenol A, And after elution, it is detected by high performance liquid phase; the specific steps are:

[0031] (1) Synthesis of 100nm magnetic nanoparticles

[0032] 100nm magnetic nanomaterials (Fe 3 o 4 ) were synthesized by hydrothermal method. Accurately weigh 2g of ferric chloride hexahydrate and 4.8g of sodium acetate and dissolve them in 60mL of ethylene glycol, then add 1.5g of polyethylene glycol and fully stir for 2 hours under the action of a mechanical stirrer, transfer all the yellow liquid to a container with polyethylene glycol In a sealed stainless steel hydrothermal reaction kettle with a tetrafluoroethylene liner (100 mL), heat and react in an oil bath at 210° C. for 12 h. After the reaction was complete...

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Abstract

The invention discloses a method for detecting [2,2-bis(4-hydroxyphenol)propane] based on aptamer functional magnetic nanomaterial, belonging to the field of food safety testing. The method is used for detecting the content of [2,2-bis(4-hydroxyphenol)propane] in milk, bottled mineral water and the like. According to the method, [2,2-bis(4-hydroxyphenol)propane] specific nucleic acid aptamer functional magnetic nanomaterial and the enrichment effect of a sample treated by magnetic separation through the nanomaterial are utilized, and the target substance in the sample is rapidly concentrated, so that the detection sensitivity is improved. Furthermore, high performance liquid chromatography is combined with the characteristic of high sensitivity of and fluorescence detection, and the recovery rate of eluant absorbed by magnetic nanoparticles can be measured by the high performance liquid chromatography.

Description

technical field [0001] The invention belongs to a method for detecting organic matter, and in particular relates to a method for detecting bisphenol A using DNA as an adsorption aptamer—a method for detecting the recovery rate of bisphenol A adsorbed by an aptamer functionalized magnetic nanometer material. Background technique [0002] Bisphenol A [2,2-bis (4-hydroxyphenol) propane, BPA] is the main raw material for the production of various polymer materials such as polycarbonate and epoxy resin, and can also be used in fine chemicals such as antioxidants, coatings, and pesticides product production. Bisphenol A residues in food are mainly through food raw materials and food packaging: [0003] (1) Bisphenol A is difficult to degrade in the environment and widely exists in nature, especially in environmental water and plastic products, and is enriched in organisms, and enters our food through the food chain. [0004] (2) Since bisphenol A is fat-soluble, in products such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 王利兵丁利龚强成婧许宙唐瑶朱绍华
Owner 王利兵
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