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Preparation method of pepsin and trypsin treated Liuwei Dihuang composite micro-ecological oral liquid

A technology of compound microecology and trypsin, which is applied in the field of preparation of classic Liuwei Dihuang pills and Liuwei Dihuang compound microecological oral liquid by using biotechnology to achieve the effect of reducing side effects

Inactive Publication Date: 2015-06-03
白杨
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the above-mentioned patent can extract and concentrate the medicinal ingredients in Liuwei Dihuang Pills to produce concentrated Liuwei Dihuang Pills, the side effects of Liuwei Dihuang Pills that hinder digestion have not been weakened

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] The preparation steps of the tomato extract are as follows:

[0042] Wash and weigh fresh tomatoes, chop them up, add an equal amount of distilled water, heat in a water bath at 100°C, stir for 90 minutes, then filter with gauze to adjust the pH to 7.0;

[0043] The preparation steps of the liver extract are as follows:

[0044] Weigh 1000g of fresh pork liver, cut into small pieces or mince, add distilled water to 2000mL, mix well, put in the refrigerator overnight, boil for 15min the next day, filter with gauze and squeeze to collect the filtrate, add water to make up to 2000mL.

[0045] The MRS liquid culture medium in the embodiment has every liter of components as follows:

[0046] Peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate [(NH 4 ) 2 HC 6 h 5 o 7 ] 2.0g, glucose (C 6 h 12 o 6 ·H 2 (O) 20.0g, Tween 801.0mL, sodium acetate (CH 3 COONa·3H 2 O) 5.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0g, magnesi...

Embodiment 1

[0048] A preparation method of Liuwei Dihuang compound microecological oral liquid treated with stomach and trypsin, the steps are as follows:

[0049] (1) After pulverizing the Liuwei raw material medicine in Liuwei Dihuang Wan raw materials, the raw material powder is obtained, and then 200 grams of water and refined honey are added for every 100 grams of raw material powder, and the raw material slurry is obtained by mixing evenly;

[0050] (2) Add hydrochloric acid to the raw material slurry prepared in step (1) to adjust the pH value to 1.5, add 20 activity units of pepsin per 100 grams of Liuwei Dihuang Wan raw material, treat at 36°C for 3 hours, adjust with sodium carbonate The pH value is 8, and the pepsin-treated slurry is obtained;

[0051] (3) Add 50 activity units of trypsin per 100 ml to the pepsin-treated slurry prepared in step (2), treat at a temperature of 37° C. for 2 hours, and adjust the pH value to 5.5 with hydrochloric acid to obtain stomach, Trypsinize...

Embodiment 2

[0056] A preparation method of Liuwei Dihuang compound microecological oral liquid treated with stomach and trypsin, the steps are as follows:

[0057] (1) After pulverizing the Liuwei raw material drug in the Liuwei Dihuang Wan raw material, the raw material powder is obtained, and then 600 grams of water and refined honey are added for every 100 grams of the raw material powder, and the raw material slurry is obtained by mixing evenly;

[0058] (2) Add acid to the raw material slurry prepared in step (1) to adjust the pH value to 2.2, add 60 activity units of pepsin per 100 grams of Liuwei Dihuang Wan raw material, and treat it for 2 hours at a temperature of 38 ° C, and adjust it with sodium carbonate. The pH value is 7.8, and the pepsin-treated slurry is obtained;

[0059] (3) Add 20 activity units of trypsin per 100 ml to the pepsin-treated slurry prepared in step (2), treat at a temperature of 37° C. for 3 hours, and adjust the pH value to 6 with hydrochloric acid to obt...

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Abstract

The invention relates to a preparation method of pepsin and trypsin treated Liuwei Dihuang composite micro-ecological oral liquid. The preparation method comprises the following steps: (1) crushing Liuwei Dihuang pill raw materials, adding water, and mixing uniformly to prepare raw material serous fluid; (2) adding acid into the raw material serous fluid to adjust the pH value, and adding pepsin for treatment, thereby preparing pepsin treated serous fluid; (3) adding trypsin into the pepsin treated serous fluid for treatment to prepare the pepsin and trypsin treated serous fluid; and (4) inoculating bifidobacteria and lactobacillus plantarum to obtain the pepsin treated Liuwei Dihuang composite micro-ecological oral liquid. The pepsin and trypsin treated Liuwei Dihuang composite micro-ecological oral liquid prepared by the preparation method provided by the invention is relatively small in side effect, and is palatable in sweet and sour tastes; and by virtue of practical application, after being taken for a long time, the pepsin and trypsin treated Liuwei Dihuang composite micro-ecological oral liquid not only can achieve a treatment effect of Liuwei Dihuang pills but also greatly reduces the side effects causing stomach cold and digestion obstruction.

Description

technical field [0001] The invention relates to a preparation method of Liuwei Dihuang compound micro-ecological oral liquid treated with stomach and trypsin, in particular to a method for processing the classic Liuwei Dihuang pill prescription by using biotechnology, which belongs to the technical field of biomedicine. Background technique [0002] Liuwei Dihuang Wan originated from Jinkui Shenqi Wan in "Synopsis of the Golden Chamber" by Zhang Zhongjing, a great medical scientist in the Eastern Han Dynasty. It is also called "Shenqi Wan" and "Bawei Dihuang Wan". Qian Yi, the national imperial physician, subtracted cinnamon and aconite from the original prescription to form Liuwei Dihuang, which is composed of Rehmannia glutinosa, Cornus officinalis, Chinese yam (three tonics), Poria cocos, Alisma, and Moutan cortex (three tonics). Pills; three tonics and three diarrhea, tonic but not dry. Liuwei Dihuang Wan has been formulated for more than 1,000 years since the Song Dyna...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8945A61P1/14
Inventor 白杨
Owner 白杨
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