A method of using Aspergillus niger to decompose phosphate rock powder to restore u(vi) polluted water
A technology of phosphate rock powder and Aspergillus niger, applied in the field of U-polluted water bodies, can solve the problems of secondary environmental pollution, secondary treatment difficulties, high treatment costs, etc., and achieve the effects of reduced alkali consumption, good repair effect, and simple process
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[0023] Prepare the improved Montina inorganic phosphorus bacteria culture medium, the main component is 5-10 g glucose (C 6 h 12 o 6 ), 0.3-0.5 g ammonium sulfate [(NH 4 ) 2 SO 4 ], 0.2-0.3 g sodium chloride (NaCl), 0.2-0.3 g magnesium sulfate (MgSO 4 ·7H 2 O), 0.02-0.03 g ferrous sulfate (FeSO 4 ), 0.02-0.03 g manganese sulfate (MnSO 4 ·H 2 O), 0.1-0.3 g potassium chloride (KCl), 1-10 g phosphate rock powder (phosphorus content above 3%). Add 10-15 g of Montina inorganic phosphorus bacteria culture medium per liter of U(VI) polluted water, and adjust the pH value to 6.0-8.0 with 2 mol / L NaOH and 1:1 HCl solution. Sterilize the U(VI) polluted water body added with Montina inorganic phosphorus bacteria medium at 121°C for 30 minutes, inoculate 10-50 ml / L of Aspergillus niger spore suspension with OD value of 0.05-0.2 into U (VI) In polluted water bodies. After inoculation, at 25-30°C, culture with shaking or aerobic culture. During the cultivation process, when the ...
Embodiment 1
[0027] Example 1, in 1 liter of U(VI) polluted water with a concentration of 50 mg / L, 15 g of Montina medium with a phosphate rock powder content of 2 g / L was added to adjust the pH value to 6.0. 121°C After sterilizing under high temperature for 30 min, cool to room temperature, inoculate 10 ml of Aspergillus niger spore suspension with OD value of 0.1, and incubate at 25 °C for 96 h. At this time, the concentration of U(VI) in the polluted water dropped to 0.042 mg / L, and the pH value dropped to 4.1. After filtering with qualitative filter paper, use saturated lime milk solution to adjust the pH value of the filtrate to 7.0 before discharging.
Embodiment 2
[0028] Example 2, in 1 liter of U(VI) polluted water with a concentration of 1 mg / L, 13 g of Montina medium with a phosphate rock powder content of 2 g / L was added to adjust the pH value to 6.0. 121°C After sterilizing for 30 min, cool to room temperature, inoculate 10 ml of Aspergillus niger spore suspension with an OD value of 0.1, and incubate at 25 °C for 84 h. At this time, the concentration of U(VI) in the polluted water dropped to 0.036 mg / L, and the pH value dropped to 4.5. After filtering with qualitative filter paper, use saturated lime milk solution to adjust the pH value of the filtrate to 7.0 before discharging.
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