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Method for cultivating strawberry seedlings by secondary detoxification method

A strawberry and seedling technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of reducing the yield of detoxified seedlings, low adventitious bud induction rate, and reducing detoxification efficiency, etc., and achieve high-quality commercial fruit The effect of high efficiency, strong growth and high quality

Active Publication Date: 2015-06-10
SICHUAN AAS HORTICULTURE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difficulty in conventional shoot tip culture is that the dissected shoot tip tissue (greater than 5 mm) is too large to induce, and the inoculated explants may still be infected with a small amount of virus, thereby reducing the virus-free efficiency
However, using a smaller volume of shoot tip tissue (less than 0.1 mm) for induction reduced the yield of virus-free seedlings due to the lower rate of adventitious bud induction

Method used

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  • Method for cultivating strawberry seedlings by secondary detoxification method
  • Method for cultivating strawberry seedlings by secondary detoxification method
  • Method for cultivating strawberry seedlings by secondary detoxification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Select strawberry stolons, wrap it in moist newspaper, and store temporarily at 4-5℃;

[0043] (2) Sterilization: Sterilize with 75% medical ethanol for 1 min, wash with sterilized water twice, 1 min / time, and remove excess water; then sterilize with 0.1% mercury liters for 2 min, wash with sterilized water twice, 1 min / time, then remove the excess water; ethanol and mercury are used to disinfect the surface of the tissue. After removing the excess water, it is inoculated on the surface of the culture medium, so that the induced shoot tip tissue can fully contact the culture medium.

[0044] (3) Preparation medium: adventitious bud induction medium, strawberry multiplication medium and rooting medium;

[0045] Adventitious bud induction medium contains Ms medium, 6BA 1.5 mg / L, NAA 0.05 mg / L, sucrose 30 g / L, and agar powder 6 g / L;

[0046] Strawberry proliferation medium contains Ms medium, 6BA 0.3-0.8 mg / L+IBA 0.05 mg / L, hydrolyzed casein 0.05 mg / L, sucrose 15 g / L, and agar...

Embodiment 2

[0053] The method for cultivating strawberry seedlings by the two-step detoxification method includes the following steps:

[0054] (1) Select "Fengxiang" strawberry stolons, wrap it with moist newspaper to keep the tissue from dehydrating, and bring it back to the laboratory for short-term storage at 4°C;

[0055] (2) Sterilization: Sterilize with 75% medical ethanol for 1 min on the ultra-clean workbench, then wash 3 times with sterilized ultrapure water (18.3MΩ*cm), 1 min / time; place on sterile filter paper, Absorb excess water; sterilize with 0.1% mercury liter for 3 min, then wash with sterilized ultrapure water 3 times, 1 min / time, and place on sterilized filter paper to absorb excess water. Among them, medical ethanol and mercury are used to disinfect the surface of the tissue. After the absorbent paper absorbs the excess water, the tissue is inoculated on the surface of the culture medium to allow it to fully contact the culture medium.

[0056] (3) Preparation of culture me...

Embodiment 3

[0065] The method for cultivating strawberry seedlings by two-step detoxification method includes the following steps:

[0066] (1) Select "Fengxiang" strawberry stolons, wrap it with moist newspaper to keep the tissue from dehydrating, and bring it back to the laboratory for short-term storage at 4°C;

[0067] (2) Sterilization: Sterilize with 75% medical ethanol for 1 min on the ultra-clean workbench, then wash 3 times with sterilized ultrapure water (18.3MΩ*cm), 1 min / time; place on sterile filter paper, Absorb excess water; sterilize with 0.1% mercury liter for 3 min, then wash with sterilized ultrapure water 3 times, 1 min / time, and place on sterilized filter paper to absorb excess water. Among them, medical ethanol and mercury are used to disinfect the surface of the tissue. After the absorbent paper absorbs the excess water, the tissue is inoculated on the surface of the culture medium to allow it to fully contact the culture medium.

[0068] (3) Preparation of culture medium...

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Abstract

The invention belongs to the field of strawberry seedling propagation, and particularly relates to a method for cultivating strawberry seedlings by a secondary detoxification method. The method is characterized by comprising the following steps: selecting meristem of shoot tips of creeping stems of strawberries, detoxifying for the first time, detoxifying the induced meristem of stems and tips of test-tube plantlets for the second time and the like. The method is used for detoxifying twice by utilizing meristem of shoot tips of creeping stems of strawberries and the induced meristem of stems and tips of test-tube plantlets, and a core stock plant is cultivated. The cultivated detoxified strawberry seedlings are strong in growth vigor, are improved in fruit set percentage, and are high in yield, excellent in quality and economic benefit.

Description

technical field [0001] The invention belongs to the field of strawberry seedling breeding, in particular to a method for cultivating strawberry seedlings by a two-step detoxification method. Background technique [0002] strawberry( Fragaria X ananassa ) is a perennial herb of the genus Strawberry in the Rosaceae family. In recent years, strawberry planting area has developed rapidly, and the world's total output has jumped to the second place in berry fruits. my country is the country with the largest distribution of strawberry species in the world, and it is also the country with the largest strawberry cultivation area and the largest output in the world. Strawberry long-term asexual reproduction and continuous cropping lead to species degeneration and plant infection of viral diseases, often manifested as plant dwarfing, reduced stress resistance, increased deformed fruit, decreased fruit quality, and reduced yield per unit area. Strawberry virus diseases are widely ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王建辉刘建军陈克玲李洪雯何建关兵何礼
Owner SICHUAN AAS HORTICULTURE RES INST