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High molecular weight (HMW) pleurotus eryngii polysaccharide and preparation method thereof

A technology of polymer and Pleurotus eryngii leftovers, which is applied in the field of extraction of active ingredients of edible fungi, can solve problems such as less research, and achieve the effects of simple process, reduced environmental pollution, and rich sources of raw materials

Inactive Publication Date: 2015-06-17
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on Pleurotus eryngii polysaccharides with a molecular weight of millions of Daltons

Method used

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  • High molecular weight (HMW) pleurotus eryngii polysaccharide and preparation method thereof
  • High molecular weight (HMW) pleurotus eryngii polysaccharide and preparation method thereof
  • High molecular weight (HMW) pleurotus eryngii polysaccharide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1 Crude polysaccharide extraction: Weigh 500g of Pleurotus eryngii leftovers (Shanghai Bei'an Fungus Professional Cooperative), add 6.5L of water, bath in boiling water for 2h, filter, centrifuge the filtrate at 10000r / min for 15min, add water to the residue 6L, bath in boiling water for 1h , filtered, the filtrate was centrifuged at 10000r / min for 15min, and the supernatants extracted twice were combined.

[0025] 2 Ultrafiltration of supernatant: The ultrafiltration equipment is a nanofiltration ultrafiltration reverse osmosis integrated machine (BH-UF-NF-RO-3-2012, Shanghai Beihong Biotechnology Co., Ltd.), using a roll-type ultrafiltration membrane of 100kDa (model PSI1, Ande Membrane Separation Technology Engineering Co., Ltd.), the ultrafiltration pressure is 0.15-0.2MPa, the ultrafiltration temperature is room temperature, and the ultrafiltration time is 2h. The ultrafiltration was terminated when the final conductivity was less than 80 μs / cm, and the retentate a...

Embodiment 2

[0030] 1 Crude polysaccharide extraction: Weigh 500g of Pleurotus eryngii offal pieces (Shanghai Beian Fungus Industry Professional Cooperative), add 7L of water, bath in boiling water for 2h, filter, centrifuge the filtrate at 10000r / min for 15min, add 6L of water to the residue, bath in boiling water for 1h, After filtering, the filtrate was centrifuged at 10,000 r / min for 15 min, and the supernatants extracted twice were combined.

[0031] 2 Supernatant ultrafiltration: The ultrafiltration equipment is a nanofiltration ultrafiltration reverse osmosis integrated machine (BH-UF-NF-RO-3-2012, Shanghai Beihong Biotechnology Co., Ltd.), using a roll-type ultrafiltration membrane of 100kDa, ultrafiltration The filtration pressure is 0.15-0.2MPa, the ultrafiltration temperature is room temperature, and the ultrafiltration time is 2h. During this period, 3 times the volume of distilled water is added to dilute the supernatant, so that the final conductivity of the liquid passing thr...

Embodiment 3

[0036] 1. Sample preparation: Weigh 2 mg of the high molecular weight Pleurotus eryngii polysaccharide prepared in Example 1, add 1 mL of mobile phase (containing 0.05 mol / L of NaH 2 PO 4 and 0.15mol / L NaNO 3 solution), fully dissolved, centrifuged at 13000r / min for 10min, and the supernatant was filtered through a 0.45μm aqueous microporous membrane (Shanghai Anpu Scientific Instrument Co., Ltd.) for HPSEC-MALLS-RI analysis.

[0037] 2. Chromatographic analysis conditions: HPSEC-MALLS-RI System combined with HPSEC-MALLS-RI System: Waters2695 HPLC, helium-neon The eight-angle laser light scattering detector (MALLS, Wyatt Company) of the laser light source and the Waters 2414 differential detector (RI) are composed. The analytical column is TSK PWXL 6000 and TSK PWXL 4000 gel chromatographic column (TOSOH company) connected in series for analysis, and the mobile phase is NaH containing 0.05mol / L 2 PO 4 and 0.15mol / L NaNO 3 solution (pH=7, 0.02% sodium azide), the flow rate...

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Abstract

The invention discloses high molecular weight (HMW) pleurotus eryngii polysaccharide and a preparation method thereof. The HWM pleurotus eryngii polysaccharide is prepared by the following method: crude polysaccharide extraction, supernate ultrafiltration, low-concentration alcohol precipitation, alcohol washing and drying. The prepared pleurotus eryngii polysaccharide has the advantage that the molecular weight, the purity and the activity are high.

Description

technical field [0001] The invention belongs to the field of extracting active ingredients from edible fungi, and in particular relates to a high-molecular-weight Pleurotus eryngii polysaccharide and a preparation method thereof. Background technique [0002] Modern pharmacological studies have shown that polysaccharides in Pleurotus eryngii (Pleurotus Eryngii) are one of the main active ingredients, and have significant pharmacological effects in antithrombosis, blood lipid regulation, immune function regulation, antitumor, and antiradiation. At present, there are many studies on the activity of Pleurotus eryngii crude polysaccharides, and the research on pure polysaccharides is mainly concentrated on the molecular weight of 10,000 to hundreds of thousands of Daltons. For example, Zhang Huapeng et al. and Sepharose-G150 gel column chromatography to obtain Pleurotus eryngii polysaccharides WPP1 and WPP2, and their average molecular weights are 2.871×10 4 Da and 4.499×10 5 ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘艳芳薛令坤张劲松唐庆九周帅杨焱吴迪颜梦秋张忠冯娜贾薇冯杰唐传红汪雯翰
Owner SHANGHAI ACAD OF AGRI SCI
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