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Beauveria bassiana barrel mixed agent and preparation method thereof

A technology of Beauveria bassiana and tank mixture, which is applied in the fields of botanical equipment and methods, insecticides, biocides, etc., can solve the problem of low efficacy of new formulations, degradation of strain virulence, and low labor efficiency in application, etc. problems, to achieve the effect of promoting commercialization and industrialization development, long shelf life, and high efficiency of spraying operations

Inactive Publication Date: 2015-07-01
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Beauveria bassiana is a microbial pesticide and the most widely used entomopathogenic fungus in China. It can infect more than 800 species of pests in the family Lepidoptera, Mothidae, Chrysopidae, Scarabidae, and Aphididae. It has the advantages of strong pathogenicity, wide insecticidal range, and environmental friendliness. It has been widely used in various regions, but there are still disadvantages in the control of single dosage forms, low labor efficiency, slow insecticidal speed, and low insecticidal efficiency.
At present, the most successful promotion model of Beauveria bassiana in my country is the control of corn borer and pine caterpillar, but they are all promoted by the government. Bacteria granules are also used, but the labor efficiency is low and has gradually disappeared
The main reasons for the difficulty in the promotion and commercialization of Beauveria bassiana in my country are the degradation of strain virulence, single dosage form, difficult preservation of dosage form, and low operating efficiency.
For a long time, the development of new dosage forms of Beauveria bassiana has been difficult, mainly because: Beauveria bassiana is a living microorganism that exerts its medicinal effect, and the process of developing the dosage form can easily lead to the inactivation of the strain, resulting in the development of new dosage forms with low efficacy and no promotion. value; the new dosage form of Beauveria bassiana has always been powder-based, and the labor efficiency of application is low. It is urgent to develop a new dosage form with high drug efficacy and convenience for application; even if an effective dosage form is developed, it is difficult to preserve the dosage form and use it in the field. The promotion and use of Beauveria bassiana are limited, so it is difficult to promote and commercialize Beauveria bassiana

Method used

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  • Beauveria bassiana barrel mixed agent and preparation method thereof
  • Beauveria bassiana barrel mixed agent and preparation method thereof
  • Beauveria bassiana barrel mixed agent and preparation method thereof

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Experimental program
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Embodiment

[0027] (1) Experimental content

[0028] The preparation method of the Beauveria bassiana tank mix comprises the following steps: after uniformly mixing 10.0-20.0 g of powder and 50.0 ml of additives, adding 10000-20000 ml of water and stirring evenly to obtain the Beauveria bassiana tank mix. Water is pure water.

[0029] The composition of the Beauveria bassiana tank mix of each embodiment of table 1

[0030]

[0031] Powders 1-7 (see Table 2) were sand-milled and mixed for 10 minutes at 4000rpm in proportion, 10%≥humidity≥5%, stored in the dark at -20°C, 4°C and room temperature, and the spore germination rate was regularly detected every month. Periodically detect the activity of pr1 protease and the virulence of corn borer larvae, and screen the appropriate powder combination.

[0032] The composition of each powder of table 2

[0033]

[0034] Additives 1-3 (see Table 3) were sterilized by irradiation with Co 60 rays, and stored at room temperature and protected...

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Abstract

The invention discloses a beauveria bassiana barrel mixed agent and a preparation method of the beauveria bassiana barrel mixed agent. The beauveria bassiana barrel mixed agent comprises the components in parts by weight: 10.0-20.0g of powder, 50.0ml of an assistant and 10,000-20,000ml of water; the powder comprises the components in parts by weight: 90.0-100.0g of beauveria bassiana high spore, 0-10.0g of filler, 0-2.0g of a high-temperature protective agent, 0-3.0g of a nutrition thickener, 0-0.5g of stable protective agent, 0-0.2g of antioxidant, 0-0.5g of a biological protective agent, 0-1.0g of an ultraviolet protective agent A and 0-0.5g of a biological regulating agent; the assistant comprises the components in parts by weight: 10.0g of a dispersing agent, 15.0-25.0g of an emulsification dispersing agent A, 15.0-25.0g of an emulsification dispersing agent B, 5.0g of an ultraviolet protective agent B and 30ml of water. According to the beauveria bassiana barrel mixed agent and the preparation method of the beauveria bassiana barrel mixed agent, the pesticide efficiency is guaranteed, the long shelf life and the high efficiency of the pesticide application operation can be realized, and the commercialization and industrialization of beauveria bassiana can be promoted.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, in particular to a beauveria bassiana tank mix and a preparation method thereof. Background technique [0002] Corn is the second largest grain crop in my country, with an annual output of more than 300 billion catties. It can be used for food, and can also be used as feed and industrial raw materials to produce industrial alcohol and shochu. The corn borer (Ostrinia nubilalis) is the main pest in corn production. It not only eats the leaves of corn, bores into the main stem or ear of corn, reduces photosynthesis, affects nutrient transport, but also produces various secondary diseases, resulting in reduced corn yields. The quality of maize causes a loss of 7%-15% of the yield of corn all the year round, and more than 50% of the yield reduction in serious years, or even extinction, causing huge economic losses to my country's grain production. [0003] Chemical pesticides have always...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/22A01N25/02A01P7/04A01P21/00A01N61/00
Inventor 徐文静李启云隋丽张正坤王义生王广祥解娇张佳诗路杨田志来任金平高月波
Owner JILIN ACAD OF AGRI SCI
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