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Flash Extraction Method of Alkaloids from Lotus Seed Heart

A technology for extracting lotus seed alkaloids and flash extraction, which is applied in the direction of alkaloids, alkaloids, chemical instruments and methods, etc., can solve the problems of high energy consumption and long extraction time, and achieve energy saving, short extraction time and low production cost low effect

Active Publication Date: 2017-10-13
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a production method of lotus seed heart extract, which overcomes the disadvantages of long extraction time and large energy consumption in the existing extraction method

Method used

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  • Flash Extraction Method of Alkaloids from Lotus Seed Heart
  • Flash Extraction Method of Alkaloids from Lotus Seed Heart
  • Flash Extraction Method of Alkaloids from Lotus Seed Heart

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Method for flash extraction of lotus seed heart alkaloids

[0017] (1) Preparation method

[0018] 1) Pretreatment of raw materials: dry the lotus seed heart at 40°C for 4 hours, take 20g in a beaker;

[0019] 2) Extraction solvent: Measure 200ml of 60% (v / v) ethanol solvent;

[0020] 3) Flash extraction: put the extraction solvent in the beaker of step 1), use a flash extractor to perform mechanical pulverization extraction for 3 minutes, and filter the suspension to obtain the filtrate;

[0021] 4) Concentration: Concentrate the filtrate to obtain an extract;

[0022] 5) Purification: Suspend the extract obtained in 4) with water, extract with n-hexane, discard the n-hexane layer, extract the water layer with chloroform, and dry the chloroform layer under reduced pressure to obtain total alkaloids of lotus root.

[0023] (2) Detection

[0024] Dissolve the obtained total alkaloids of Lianxin in water, and make it up to 50ml, take 40μl from it and put it ...

Embodiment 2

[0025] Embodiment 2 The influence of different extraction solvents

[0026] (1) Preparation method:

[0027] 1) Pretreatment of raw materials: dry the lotus seed heart at 40°C for 4 hours, take 20g in a beaker;

[0028] 2) Extraction solvent: Measure 200ml of n-butanol, water, ethanol, methanol, acetone, ethyl acetate respectively;

[0029] 3) Flash extraction: put the extraction solvent in the beaker of step 1), use a flash extractor to perform mechanical pulverization extraction for 3 minutes, and filter the suspension to obtain the filtrate;

[0030] (2) Detection

[0031] Dissolve n-butanol extract, water extract, ethanol extract, methanol extract, acetone extract and ethyl acetate extract in water respectively, and dilute to 50ml, take 40μl and dilute to 10ml, take a small amount and use the aperture Filter it with a microporous membrane of 0.22 μm, use Agilent1290 and Agilent6460 triple quadrupole LC-MS, and use liensinine, isoliensinine, and methyl-liensinine as eval...

Embodiment 3

[0035] Embodiment 3 The influence of extraction solvent concentration

[0036] (1) Preparation method: the method is the same as in Example 1, except that the concentration of the ethanol solvent is changed as shown in Table 2.

[0037] (2) Detection

[0038] 1. Determination of absorbance: Dissolve ethanol extracts of different concentrations in water, set the volume to 50ml, take 40μl and set the volume to 10ml, take a small amount, measure the absorbance at 282nm with Shimadzu 2550, the results are shown in Table 2.

[0039] Table 2

[0040] Volume fraction%

0

20

40

50

60

70

80

Absorbance

0.214

0.280

0.284

0.334

0.410

0.367

0.299

[0041] It can be seen from Table 2 that different concentrations of ethanol have different extraction effects. The absorbance of 60% ethanol is the largest, and the extract has no obvious green color. The absorbance of 70% and 80% ethanol extracts is small, and the extract becomes o...

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Abstract

The invention relates to a method for producing a lotus plumule extractive. The method comprises the following steps: mixing the preprocessed raw material and an extracting solvent, extracting for 2-8 min by a flash extractor, and filtering a suspension solution to obtain a filtrate, wherein the extracting solvent is water and / or ethanol; preferably the extracting solvent is an ethanol solution with the volume percentage concentration of 50-70%; drying the filtrate to obtain an extractum; and adding water to the extractum, suspending, extracting by normal hexane, removing a normal hexane layer, extracting a water layer by trichloromethane, and performing reduced-pressure distillation drying on the trichloromethane layer to obtain the lotus plumule total alkali. The method provided by the invention is simple to operate and short in extracting time; the extracting time is not greater than 4% of the traditional heating, refluxing and extracting time; the sample posttreatment is convenient, the energy resource is saved, the production cost is low and the period is short, so that the method is suitable for large-scale production. The liensinine, isoliensinine and methyl liensinine can be extracted and obtained through the method.

Description

technical field [0001] The invention belongs to the field of methods for extracting active ingredients from Chinese herbal medicines, in particular to a method for extracting alkaloids from lotus seeds. Background technique [0002] The lotus seed heart is the green radicle in the middle of the mature seeds of the Nymphaeaceae plant lotus. It is mainly produced in Hunan, Hubei, Jiangxi, Fujian, Jiangsu, Zhejiang and other places. Nature and flavor are bitter, cold. Clear heart, remove heat, stop bleeding, astringent essence. Cure upset, thirst, vomiting blood, nocturnal emission, and calm the five internal organs. Studies have shown that it contains alkaloids such as liensinine, isoliensinine, methyl-liensinine, nuciferine, pronuciferine, ceratine, methyl corydine paling, desmethyl citrine, imipapaverine, etc. , and contains flavonoids such as rhinoside, hyperoside and rutin. Studies have shown that the alkaloid components in it have powerful pharmacological effects suc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D217/20C07G5/00
CPCC07D217/20C07G5/00
Inventor 陈长兰吕晶王帅孙婵孟雪莲邱马超王琦赵晓宇
Owner LIAONING UNIVERSITY
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