A broadspectrum efficient antimicrobial peptide Pb-CATH-OH1, a gene thereof, a preparing method of the peptide and applications of the peptide

An antimicrobial peptide, pb-cath-oh1 technology, applied in the field of biomedicine to achieve the effects of good killing effect, simple structure and strong antibacterial activity

Active Publication Date: 2015-07-08
DALIAN UNIV OF TECH
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  • A broadspectrum efficient antimicrobial peptide Pb-CATH-OH1, a gene thereof, a preparing method of the peptide and applications of the peptide
  • A broadspectrum efficient antimicrobial peptide Pb-CATH-OH1, a gene thereof, a preparing method of the peptide and applications of the peptide
  • A broadspectrum efficient antimicrobial peptide Pb-CATH-OH1, a gene thereof, a preparing method of the peptide and applications of the peptide

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[0020] Specific embodiments of the present invention will be described in detail below in conjunction with technical solutions, but the content of the present invention is not limited thereto.

[0021] Strictly follow the kit instructions, using RNeasy AxyPrep TM Multisource Total RNA Miniprep Kit (Qiagen, union city, CA, USA) was used to extract the total RNA from Burmese python lung tissue, and then use Creator TM SMART TMThe cDNA Library Construction Kit was used to construct the cDNA library of Burmese python lung tissue. Use the PowerScript Reverse Transcriptase in the kit to reverse transcribe and synthesize the first strand of cDNA. The primers are:

[0022] Forward SMARTⅣOligonucleotide primer:

[0023] 5'–AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG–3'

[0024] Reverse CDSⅢ / 3'PCR primer: 5'–ATTCTAGAGGCCGAGGCGGCCGACATG-d(T) 30 N -1 N–3’ (N=A, C, G, or T; N -1 = A, G, or C).

[0025] Use Advantage DNA Polymerase to synthesize the second strand of cDNA. The primer ...

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Abstract

A broadspectrum efficient antimicrobial peptide Pb-CATH-OH1, a gene thereof, a preparing method of the peptide and applications of the peptide are disclosed. The antimicrobial peptide Pb-CATH-OH1 is a straight-chain polypeptide. The sequence of the antimicrobial peptide Pb-CATH-OH1 is as follows: lysine-arginine-phenylalanine-lysine-lysine-phenylalanine-phenylalanine-arginine-lysine-isoleucine-lysine-lysine-glycine-phenylalanine-arginine-lysine-isoleucine-phenylalanine-lysine-lysine-threonine-lysine-isoleucine-phenylalanine-isoleucine-glycine-glycine-threonine-isoleucine-proline-isoleucine. A precursor gene comprises 528 nucleotides, and nucleotides from the number 433 site to the number 525 site code the antimicrobial peptide. The antimicrobial peptide is rich in basic amino acids, low in molecular weight, simple in structure, extremely high in broadspectrum antimicrobial activity, low in hemolysis and not liable to generate drug resistance and has activity for gram-positive bacteria, gram-negative bacteria and fungi. The antimicrobial peptide can be used as an additive for preparation of medicines, cosmetics, foods, feed, and the like which are resistant to pathogenic microorganism infection.

Description

technical field [0001] The present invention provides a cathelicidin family broad-spectrum antimicrobial peptide Pb-CATH-OH1 derived from Burmese python (Python molurus bivittatus) and its encoding gene, amino acid sequence and its daily chemical additives in cosmetics, health products, food, feed and the like. The application in the field of anti-infection medicine belongs to the field of biomedical technology. Background technique: [0002] Cathelicidin is a kind of multifunctional host antibacterial peptide family composed of N-terminal signal peptide region, intermediate conservative cathelin domain and C-terminal highly specific mature peptide region. The structural characteristics are: N-terminal signal peptide region (30 residues bases), an intermediate conserved cathelin domain (99-114 residues) and a highly specific mature peptide region at the C-terminus (12-100 residues). Cathelicidin has broad-spectrum antibacterial activity and can quickly and broadly kill a va...

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Application Information

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IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P31/04A61K8/64A23L1/305A23K1/16
Inventor 于海宁王义鹏蔡莎莎刘雪莲王晨
Owner DALIAN UNIV OF TECH
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