Hydrazide pH sensitive polymer as well as preparation method and application thereof

A polymer and sensitive technology, applied in the field of analytical chemistry, can solve the problems of reducing the enrichment efficiency of glycoprotein or glycopeptide, and reduce the reaction rate of aldehyde group, so as to achieve the effect of improving the reaction rate and the enrichment efficiency of glycopeptide

Active Publication Date: 2015-07-08
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, due to the existence of reaction steric hindrance, the rate of its reaction with aldehyde groups in glycoprot

Method used

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  • Hydrazide pH sensitive polymer as well as preparation method and application thereof
  • Hydrazide pH sensitive polymer as well as preparation method and application thereof
  • Hydrazide pH sensitive polymer as well as preparation method and application thereof

Examples

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Embodiment 1

[0028] In the embodiment of the present invention, a hydrazide pH sensitive polymer, its raw material includes acrylic acid, methyl acrylate, potassium persulfate, hydrazine hydrate, methanol and water, the molar ratio of the acrylic acid and methyl acrylate is 1 :2, the molar ratio of the hydrazine hydrate and methyl acrylate is 1:10, the charging amount of the potassium persulfate is 0.02%w / v, and the volume ratio of the methanol and the aqueous phase is 1:3.

[0029] The preparation method of the hydrazide pH-sensitive polymer, the specific steps are as follows:

[0030] (1) Taking materials: Weigh each raw material according to the use standard;

[0031] (2) Free radical polymerization of acrylic acid and methyl acrylate: Mix methanol and water to obtain a mixed solution, fill the mixed solution with nitrogen to remove oxygen for 1.8 hours, add acrylic acid, methyl acrylate and potassium persulfate to the above In the oxygen removal solution, continue to remove oxygen for...

Embodiment 2

[0034] A kind of hydrazide pH sensitive polymer, its raw material comprises acrylic acid, methyl acrylate, ammonium persulfate, hydrazine hydrate, acetonitrile and water, and the molar ratio of described acrylic acid and methyl acrylate is 8:1, and described hydration The molar ratio of hydrazine and methyl acrylate is 5:1, the feeding amount of ammonium persulfate is 0.2% w / v, and the volume ratio of acetonitrile and water phase is 1:1.

[0035] The preparation method of the hydrazide pH-sensitive polymer, the specific steps are as follows:

[0036] (1) Taking materials: Weigh each raw material according to the use standard;

[0037] (2) Free radical polymerization of acrylic acid and methyl acrylate: mix acetonitrile and water to obtain a mixed solution, fill the mixed solution with argon to remove oxygen for 2 hours, add acrylic acid, methyl acrylate and ammonium persulfate to the above In the oxygen removal solution, continue to remove oxygen for 30 minutes, then remove t...

Embodiment 3

[0040] A kind of hydrazide pH sensitive polymer, its raw material comprises acrylic acid, methyl acrylate, azobisisobutyronitrile, hydrazine hydrate, ethanol and water, and the molar ratio of described acrylic acid and methyl acrylate is 10:1, The molar ratio of hydrazine hydrate and methyl acrylate is 8:1, the feeding amount of azobisisobutyronitrile is 1% w / v, and the volume ratio of ethanol and water phase is 1:5.

[0041] The preparation method of the hydrazide pH-sensitive polymer, the specific steps are as follows:

[0042] (1) Taking materials: Weigh each raw material according to the use standard;

[0043] (2) Free radical polymerization of acrylic acid and methyl acrylate: mix ethanol and water to obtain a mixed solution, and fill the mixed solution with nitrogen to remove oxygen for 2.2 hours, and acrylic acid, methyl acrylate and azobisisobutyronitrile Add to the above oxygen removal solution, continue to remove oxygen for 35 minutes, remove nitrogen protection, an...

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PUM

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Abstract

The invention discloses a hydrazide pH sensitive polymer. The hydrazide pH sensitive polymer comprises the following raw materials: acrylic acid, methyl acrylate, an initiator, hydrazine hydrate, an organic phase and water, wherein the molar ratio of acrylic acid to methyl acrylate is 1: 10 to 10: 1, the molar ratio of hydrazine hydrate to methyl acrylate is 1: 10 to 10: 1, the addition amount of the initiator is 0.02-2%w/v, and the volume ratio of the organic phase to a water phase is 1: 10 to 10: 1. The hydrazide pH sensitive polymer disclosed by the invention is prepared by steps of copolymerizing acrylic acid and methyl acrylate and then carrying out a hydrazine reaction through hydrazine hydrate, wherein the content of hydrazide is obviously higher than the content of hydrazide in a commercialized hydrazide material; the reaction between a hydrazide functional group in the hydrazide pH sensitive polymer and an aldehyde group in oxidized glycopeptide is covalent binding, and the enrichment specificity of glycopeptide can be provided; the hydrazide pH sensitive polymer is in a dissolved state in a system with a pH value of higher than 4, and the phase homogeneity of the reaction of the functional group hydrazide and the aldehyde group of the glycopeptide can be realized, thus increasing the reaction speed and the glycopeptide enrichment efficiency.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a hydrazide pH-sensitive polymer, a preparation method and application thereof. Background technique [0002] Protein glycosylation is the most extensive and important post-translational modification process. More than 50% of the proteins in the human body are glycosylated. Glycosylation is involved in almost all important life processes, such as fertilization, development, immune response, intercellular recognition and communication, etc. At the same time, studies have shown that the occurrence and development of various diseases are also accompanied by protein glycosylation modifications or changes in protein glycosylation modifications. Therefore, systematic qualitative and quantitative identification and analysis of glycoproteins and the development of methodology are particularly important for elucidating the role of glycosylation modifications in various phys...

Claims

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Application Information

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IPC IPC(8): C08F220/06C08F220/14C08F8/30C07K1/14
Inventor 钱小红秦伟捷白海红张万军
Owner ACADEMY OF MILITARY MEDICAL SCI
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