Method for extracting glucosamine from self-fermentation liquid

A technology of glucosamine and fermentation liquid, which is applied in the direction of amino sugar, chemical instruments and methods, sugar derivatives, etc., can solve the problems of unstable yield, high price, large amount of waste water, etc., and achieve reduction of hydrochloric acid consumption, saving consumption, The effect of reducing dosage

Inactive Publication Date: 2015-07-22
江苏赛奥生化有限公司
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Problems solved by technology

[0003] The production of glucosamine by fermentation method includes two-step process of N-acetylglucosamine fermentation and glucosamine extraction. At present, the domestic reports on the method of extracting glucosamine from the fermentation broth include: the fermentation broth is directly separated from the solid and liquid by a ceramic membrane to remove bacteria, The clear liquid is decolorized by activated carbon, desalted by electrodialysis, and concentrated by reverse osmosis to obtain a thick slurry of N-acetylglucosamine. The thick slurry of N-acetylglucosami

Method used

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Examples

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Embodiment Construction

[0046] A method for extracting glucosamine from fermentation broth, comprising the following steps:

[0047] (1) Fermentation broth flocculation and filtration

[0048] (1) Preparation of flocculant

[0049] Add 300g of flocculant sodium polyacrylate into 4L of distilled water, heat to 55-60°C, soak and swell, stir and dissolve after 2 hours, add 6L of distilled water to make it dissolve evenly, and make 10L of 3% viscous liquid. Add 3% viscous liquid to 30L of distilled water, heat to 55-60°C, stir for 1 hour, add 60L of distilled water to dissolve and dilute it, and prepare 100L of 0.3% dilution for later use;

[0050] (2) Fermentation broth flocculation

[0051]Take 1000L of fermentation broth, the fermentation yield is 92.28g / L, the dry matter content of the fermentation broth is 11.36%, and the content of N-acetylglucosamine is 92.28kg;

[0052] After the fermentation culture is terminated, the temperature of the interlayer is raised to 80°C, incubated for 10-15 minute...

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Abstract

The invention discloses a method for extracting glucosamine from a self-fermentation liquid, which comprises the following steps: fermentation liquid flocculation, filtration, double-effect concentration, crude product preparation, fine product preparation and the like. The technique is simple and convenient, and is high in yield.

Description

technical field [0001] The invention relates to a method for extracting glucosamine from fermentation broth. Background technique [0002] Glucosamine is widely used in medicine, chemical industry, food, feed and cosmetic industries. It has important physiological functions for the human body, participates in liver and kidney detoxification, has anti-inflammatory and liver-protecting effects, and can promote the synthesis of mucopolysaccharides in the human body and improve joint synovial fluid. Viscosity, improving the metabolism of articular cartilage, is regarded by the medical community as an important substance that can prevent, alleviate and treat bone and joint diseases, and has the functions of anti-aging, regulating endocrine, lowering blood fat, and lowering blood pressure. It can also be used as a low-calorie sweetener. Flavoring agents are known as the third generation health functional food additives. Glucosamine is usually prepared by hydrolysis of chitin, whi...

Claims

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Application Information

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IPC IPC(8): C07H5/06C07H1/00
Inventor 陈剑慧韩勤更周劲金峰
Owner 江苏赛奥生化有限公司
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