Preparation method and application of high-density RGD peptide modified material

Abstract

The invention discloses a preparation method and application of a high-density RGD peptide modified material. The preparation method comprises the following steps: dispersing a material, modified in the way that the surface is provided with an active group, into methylbenzene, adding a catalytic amount of 4-dimethylamino-pyridine, and slowly dropwise adding triethylamine and 2-bromoisobutyryl bromide under ice-bath, so as to prepare an initiator; adding cuprous bromide, 2,2'-dipyridyl and poly(ethylene glycol) methyl ether methacrylate into the initiator, so as to obtain a macromolecular polymer; adding the macromolecular polymer into a dimethylformamide solution containing an activating agent and 4-dimethylamino-pyridine to obtain an activated polymer brush, and adding RGD peptide for performing reaction in the dark at the room temperature for 18 hours; transferring a cell into the RGD peptide modified polymer brush, and performing reaction on a shaking table at the temperature of 4 DEG C for 20 minutes by adopting a phosphate buffer solution as a suspension liquid, so as to obtain the high-density RGD peptide modified material. The method is moderate in reaction conditions, simple, convenient and feasible in procedures, and achieves the purpose of fixing a cell on the surface of a solid material with high concentration and high density.

Description

technical field [0001] The invention relates to the field of biomedicine, which uses a polymer brush coating containing RGD peptide to modify the surface of a solid material to fix cells on the surface of the material, and specifically relates to a preparation method and application of a high-density RGD peptide modified material. Background technique [0002] Biological cell immobilization technology refers to the use of chemical or physical means to naturally fix or locate cells in a limited space area, maintain their inherent catalytic activity, and can be used repeatedly. Immobilized biological cells can continue to proliferate, dormant and die, and their activity remains stable. In addition to maintaining its original recognition, binding and catalytic activities, immobilized biological cells also have significant advantages such as easy separation, reusability and improved stability. If a solid material with an affinity with cells can be used as a biomedical material,...

AI Technical Summary

Problems solved by technology

This method is simple to operate and has little impact on cells, but the adsorption force is small and cells are easy to fall off

In the traditional cell immobilization method, when the cells are fixed by adsorption, the binding ability is generally weak, and the cells are easy to fall off; while the method of fixing cells by covalent bonds will cause great damage to the cells and cannot maintain cell activity.

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