Method for detecting rumen microorganisms of Holstein cows

Abstract

The invention provides a method for detecting rumen microorganisms of Holstein cows and belongs to methods for detecting rumen microorganisms of animals. According to the method, rumen fluid of the Holstein cows is extracted, DNA of microorganisms in the rumen fluid is extracted and types and relative quantity of microorganisms of the Holstein cows are determined through sequencing; the method comprises the following steps: (1), genome DNA extraction, (2) PCR amplification, (3) fluorescent quantitation, (4) high-throughput sequencing platform library construction, (5) high-throughput sequencing platform sequencing, (6) biological information analysis and (7) sequencing results. According to the method, the types and relative quantity of rumen microorganisms of the Holstein cows can be rapidly, comprehensively and efficiently detected, so that people can conveniently analyze types and quantity of rumen microorganisms of Holstein cows and study absorption digestion mechanisms for absorbing nourishment of rumen microorganisms. The method has the advantages as follows: detection time is shortened, the detection results are accurate and comprehensive, and types and relative quantity of all microorganisms in the Holstein cows can be accurately detected.

Description

technical field [0001] The invention relates to a method for detecting animal stomach microorganisms, in particular to a method for detecting rumen microorganisms of Holstein cows. Background technique [0002] High-throughput sequencing technology (High-throughput sequencing), also known as "next-generation" sequencing technology ("Next-generation" sequencing technology), can perform sequence determination and general reading on hundreds of thousands to several million DNA molecules in parallel at a time. Longer, shorter, etc. are signs. [0003] According to the development history, influence, sequencing principle and technology, there are mainly the following types: massively parallel signature sequencing (Massively Parallel Signature Sequencing, MPSS), polymerase cloning (Polony Sequencing), 454 pyrosequencing (454pyrosequencing), Illumina (Solexa) sequencing, ABI SOLiD sequencing, Ion semiconductor sequencing, DNA nanoball sequencing, etc. The first and second generat...

AI Technical Summary

Problems solved by technology

The first and second generation sequencing technologies take a long time to sequence, the measurement results are not accurate enough, the sequencing throughput is low, and the operation is more complicated

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