Preparation method and application of isorhamnetin surface molecularly imprinted microsphere based on nanometer titanium dioxide
A surface molecular imprinting and isorhamnetin technology, which is applied in the field of isorhamnetin surface molecularly imprinted polymer microspheres, can solve the problems of polymer microspheres embedding, low separation and processing efficiency, etc., and achieves improved adsorption performance and size. The effect of uniformity and increase of specific surface area
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Embodiment 1
[0019] Dissolve 1mmol template molecule isorhamnetin and 4mmol functional monomer 4-VP into 40ml porogen acetonitrile, ultrasonically disperse for 10min, then add 20mg TiO 2 , stirred mechanically for 2 hours, passed nitrogen for 20 minutes to deoxidize, then added 20 mmol of cross-linking agent EDMA, passed nitrogen for 20 minutes to deoxidize, added 50 mg of initiator AIBN, sealed in a vacuum state, and reacted in an oil bath at 63°C for 24 hours to obtain a solid polymer. Pass the synthesized imprinted polymer through a 200-mesh sieve, use methanol:acetic acid (V / V, 9:1) as the extraction solution, extract the polymer particles for 72 hours, and then use pure methanol for Soxhlet extraction for 24 hours, equilibrate Remove acetic acid. The obtained polymer was dried in a vacuum drying oven at 60°C for 24 hours to obtain the isorhamnetin surface molecularly imprinted polymer SMIP1. Prepared without TiO 2 The isorhamnetin molecularly imprinted polymer (MIP) was used as a bl...
Embodiment 2
[0036] Dissolve 1mmol template molecule isorhamnetin and 5mmol functional monomer AM in 20ml porogen chloroform: DMF (V / V, 10:3), ultrasonically disperse for 20min, then add 40mg TiO 2 , stirred mechanically for 2 hours, passed nitrogen for 40 minutes to deoxidize, then added 20 mmol of cross-linking agent EDMA, passed nitrogen for 20 minutes to deoxidize, added 20 mg of initiator AIBN, sealed under vacuum, and reacted in an oil bath at 63°C for 24 hours to obtain a solid polymer. Use methanol: acetic acid (V / V, 9:1) as the extraction solution to Soxhlet extract the polymer particles for 72 hours, and then use pure methanol for Soxhlet extraction for 24 hours to remove acetic acid in equilibrium. The obtained polymer was dried in a vacuum drying oven at 60° C. for 24 hours to obtain the isorhamnetin surface molecularly imprinted polymer SMIP2.
[0037] Weigh 50mg of the eluted SMIP2 into 150ml ground-mouth Erlenmeyer flasks, add 100ml of the -1 The isorhamnetin ethanol soluti...
Embodiment 3
[0048] Dissolve 1mmol template molecule isorhamnetin and 6mmol functional monomer 2-VP in 20mL acetonitrile, ultrasonically disperse for 10min, then add 30mg TiO 2 , stirred mechanically for 3 hours, passed nitrogen for 20 minutes to deoxidize, then added 30 mmol of cross-linking agent EDMA, passed nitrogen for 10 minutes to deoxidize, added initiator AIBN 10 mg, sealed under vacuum, and reacted in 60°C oil bath for 24 hours to obtain a solid polymer. Using methanol: acetic acid (V / V, 9:1) as the extraction solution, the polymer particles were Soxhlet extracted for 72 hours, and then eluted with pure methanol for 24 hours to remove acetic acid in balance. The obtained polymer was dried in a vacuum drying oven at 60° C. for 24 hours to obtain the isorhamnetin surface molecularly imprinted polymer SMIP3.
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