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Detection method for quantitatively analyzing 14 kinds of flavonoids in tobacco petals

A quantitative analysis and detection method technology, applied in the field of analysis and detection, can solve the problem of less flavonoids

Inactive Publication Date: 2015-08-26
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tobacco petals are rich in flavonoids, but there are few reports on the research and utilization of flavonoids in tobacco petals

Method used

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  • Detection method for quantitatively analyzing 14 kinds of flavonoids in tobacco petals
  • Detection method for quantitatively analyzing 14 kinds of flavonoids in tobacco petals
  • Detection method for quantitatively analyzing 14 kinds of flavonoids in tobacco petals

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0018] B. Preparation of the test solution: Accurately weigh 10.0 mg of the pretreated sample, add 1 ml of methanol-chloroform-water extraction solution and 200 μl of internal standard solution, the internal standard solution is daidzein, genistin and The aqueous solution of hesperidin, the concentration is 1.0 μ g / mL, after sonication, centrifugation, get the supernatant as the test solution;

[0019] C. Preparation of reference substance solution: Accurately weigh each standard compound, i.e. 14 standard samples and 3 internal standards daidzein, genistin and hesperidin, add methanol to make a solution containing 1 mg per 1 ml, To obtain a 1mg / ml flavonoid single-label solution, pipette 100 μl of a 1mg / ml low-concentration flavonoid single-label solution and a 10mg / ml high-concentration flavonoid single-label solution in a 100ml volumetric flask, and extract the solution with a sample, namely Methanol-chloroform-water extraction solution was prepared at constant volume to ob...

Embodiment 1

[0034] —— Quantitative analysis of flavonoids in the petals of tobacco cultivar N. alata

[0035] Experimental material: fresh freeze-dried N. alata Mature middle smoke petals

[0036] experimental method:

[0037] Accurately weigh 10.0 mg of freeze-dried tobacco petal samples, add 1 mL of extractant (methanol-chloroform-water, 5:2:2, volume ratio) and 200 μL of internal standard solution (1.0 μg / mL), sonicate for 30 min, After centrifugation, the supernatant was transferred to a liquid chromatography injection vial for analysis.

[0038]Chromatographic analysis conditions: chromatographic column, Waters BEH C18 (15 cm × 2.1 mm, 1.7 μm particle size); phase A, water; phase B, acetonitrile, both phases added 0.1% formic acid and 0.2 mmol / L ammonium acetate ;Mobility gradient, 0-1min, 10%B; 1-9min, 10%B-90%B; 9-11min, 90%B-100%B; 11-11.1min, 100%B-10%B; 11.1-13min, keep 10%B; column temperature 30℃, injection volume 2 μL, flow rate 0.25 mL / min.

[0039] Mass spectrometry ...

Embodiment 2

[0048] ——Quantitative analysis of flavonoids in petals of tobacco cultivar N. rustica

[0049] Experimental material: fresh freeze-dried N. rustica Mature middle smoke petals

[0050] experimental method:

[0051] Accurately weigh 10.0 mg of freeze-dried tobacco petal samples, add 1 mL of extractant (methanol-chloroform-water, 5:2:2, volume ratio) and 200 μL of internal standard solution (1.0 μg / mL), sonicate for 30 min, After centrifugation, the supernatant was transferred to a liquid chromatography injection vial for analysis.

[0052] Chromatographic analysis conditions: chromatographic column, Waters BEH C18 (15 cm × 2.1 mm, 1.7 μm particle size); phase A, water; phase B, acetonitrile, both phases added 0.1% formic acid and 0.2 mmol / L ammonium acetate ;Mobility gradient, 0-1min, 10%B; 1-9min, 10%B-90%B; 9-11min, 90%B-100%B; 11-11.1min, 100%B-10%B; 11.1-13min, keep 10%B; column temperature 30℃, injection volume 2 μL, flow rate 0.25 mL / min.

[0053] Mass spectrometry c...

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Abstract

The invention discloses a detection method for quantitatively analyzing 14 kinds of flavonoids in tobacco petals. The detection method comprises the following steps: pre-processing a sample, preparing a test solution, and preparing and measuring a reference solution. According to the invention, 17 kinds of flavonoids are identified from leaves and flowers of tobaccos in 2014; 9 kinds of flavonoids are identified in the tobaccos for the first time; therefore, a quantitative analysis method of 14 kinds of flavonoids is established; and the detection method disclosed by the invention has great significance for developing related researches of flavonoids in the tobacco petals.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a detection method for quantitative analysis of 14 flavonoid substances in tobacco petals. Background technique [0002] Flavonoids refer to a series of compounds in which two benzene rings are connected to each other through a central three-carbon atom. They are widely distributed in the plant kingdom and are important secondary metabolites of plants. Flavonoids have been proven in many plants to be related to plant resistance to insects and diseases. At the same time, flavonoids are a class of compounds with strong biological activity, and are natural antioxidants, which are widely used in medicine, food and other fields. For example, quercetin-3-O-rutinoside (rutin, vitamin p) is an important drug clinically used to prevent and treat diseases such as cerebral hemorrhage, hypertension, retinal hemorrhage, purple scar and acute hemorrhagic nephritis. ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 李勇逄涛师君丽陈萍
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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