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Application of a scalloped hammerhead cartilage angiogenesis inhibitory factor

An angiogenesis-inhibiting, scalloped-hammer shark technology, applied in food science, peptides, etc., can solve the problems that the application of cartilage angiogenesis-inhibiting factor in scalloped hammerhead shark has not been reported.

Active Publication Date: 2017-05-17
ZHEJIANG OCEAN UNIV
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  • Description
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Problems solved by technology

[0004] The applicant's research found that the research on the preparation of angiogenesis inhibitory factors using scalloped hammerhead cartilage as raw material is in a blank stage, and the application of scalloped hammerhead cartilage angiogenesis inhibitors in the preparation of drugs for inhibiting angiogenesis and preventing and treating tumors It has not been reported

Method used

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  • Application of a scalloped hammerhead cartilage angiogenesis inhibitory factor
  • Application of a scalloped hammerhead cartilage angiogenesis inhibitory factor
  • Application of a scalloped hammerhead cartilage angiogenesis inhibitory factor

Examples

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Embodiment

[0023] Example: A preparation method of angiogenesis inhibitory factor of scalloped hammerhead shark, the preparation process is as follows: scalloped hammerhead shark cartilage → tissue crushing → guanidine hydrochloride extraction → acetone fractional precipitation → gel filtration chromatography purification → cell membrane chromatography Purification → high performance liquid chromatography purification → angiogenesis inhibitory factor.

[0024] 1) Preparation of cartilage active protein fraction of scalloped hammerhead shark: crushed and homogenized scalloped hammerhead shark ( Sphyrna lewini ) Cartilage was added to 1.0 mol / L guanidine hydrochloride solution at a solid-to-liquid ratio of 1 g: 10 mL, extracted by shaking at 4 °C for 36 h, centrifuged at 4 °C and 10 000 r / min for 20 min, and the supernatant was taken into a dialysis bag with a molecular weight cut-off of 1 kDa, and dialyzed with Tris-HCl (pH 7.6) buffer solution at below 4°C for 24 h to obtain the crude...

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Abstract

The present invention relates to uses of a Sphyrna lewini cartilage angiogenesis inhibitory factor, namely, the Sphyrna lewini cartilage angiogenesis inhibitory factor is used to prepare health foods or functional additives for angiogenesis inhibition and tumor prevention. The amino acid sequence and ESI-MS detected molecular weight of the angiogenesis inhibitory factor is Pro-Asp-Tyr-Lys-Phe-Lys and 796.90 Da, respectively. The angiogenesis inhibitor factor can effectively inhibit angiogenesis in chick embryo chorioallantoic membrane, and significantly inhibits the expressions of three angiogenic factors including vascular endothelial growth factor, basic fibroblast growth factor and platelet derived growth factor in lung cancer tissues of mice bearing Lewis lung cancer, so that the angiogenesis inhibitor factor can be used to prepare health foods or functional additives for angiogenesis inhibition and tumor prevention.

Description

technical field [0001] The invention relates to the application of an active ingredient of shark cartilage, in particular to the application of an angiogenesis inhibitory factor of scalloped hammerhead shark cartilage. Background technique [0002] Angiogenesis is one of the important pathological features of vascular proliferative diseases such as tumors, diabetic retinopathy, rheumatoid arthritis and chronic inflammation. The anti-angiogenesis-based tumor biotherapy research has become a research hotspot of anti-tumor drugs in the past ten years. [0003] Compared with chemotherapy aimed at directly killing tumor cells, tumor angiogenesis inhibitors (Tumorangiogenesis inhibitors, TAIs) have the following unique advantages: (1) TAIs directly act on vascular endothelial cells, while anticancer drugs are affected by tissue diffusion. Due to the influence of tissue fibrosis and necrosis, the effective drug concentration in the tissue is often not reached; (2) Compared with tu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06A23L33/18A23L33/00
Inventor 王斌迟长凤赵玉勤孙坤来
Owner ZHEJIANG OCEAN UNIV
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