Lactoprotein concentrate with high thermal stability and preparation method thereof
A technology of milk protein concentrate and high thermal stability, which is applied in the direction of milk protein components, animal protein processing, protein food components, etc., can solve the problems of increasing protein denaturation, less energy and taking into account separation efficiency and protein denaturation at the same time, to prevent Effects of protein denaturation, good powder properties, and good thermal stability
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Embodiment 1
[0036] 1) Take fresh raw milk with a protein content of 3.2% (w / w), a fat content of 3.8% (w / w), a lactose content of 4.5% (w / w), an ash content of 0.7%, and heat it to 53 Centrifugal degreasing at ℃, centrifugal speed is 9000r / min, feed rate is 98L / h, adds sodium citrate and sodium pyrophosphate in above-mentioned skim milk, the proportioning of the two is 1: 1, and total addition is 0.3% ( w / w), stirred at 53°C for 8min, with a stirring rate of 80rpm, adjusted the pH value to 5.8 with 1mol / L HCl standard solution while stirring, and sterilized at 130°C for 5s;
[0037] 2) Use a 10kDa regenerated cellulose membrane to carry out ultrafiltration of the sterilized skim milk in step 1), the ultrafiltration temperature is 15°C, and the pressure is 2×10 5 Pa, the membrane flux in the ultrafiltration process is 25L m -2 h -1 , after adding deionized water, the membrane flux is 30L m -2 h -1 ,specifically:
[0038] A. MPC40: Collect the retentate after ultrafiltration and concen...
Embodiment 2
[0043] 1) Take fresh raw milk with a protein content of 3.0% (w / w), a fat content of 3.85% (w / w), a lactose content of 4.0% (w / w), and an ash content of 0.6%, and heat it to 50 Centrifugal degreasing at ℃, centrifugal speed is 8000r / min, feed rate is 95L / h, adds sodium citrate and trisodium phosphate in above-mentioned skim milk, the proportioning of the two is 3: 7, and total addition is 0.1% ( w / w), stirred at 53°C for 5min, with a stirring rate of 50rpm, adjusted the pH value to 5.5 with 1mol / L HCl standard solution while stirring, and sterilized at 140°C for 3s;
[0044] 2) Use a 10kDa regenerated cellulose membrane to carry out ultrafiltration of the sterilized skim milk in step 1), the ultrafiltration temperature is 10°C, and the pressure is 1.5×10 5 Pa, the membrane flux in the ultrafiltration process is 20L m -2 h -1 , after adding deionized water, the membrane flux is 25L·m -2 h -1 ,specifically:
[0045] A. MPC40: Collect the retentate after ultrafiltration and ...
Embodiment 3
[0050] 1) Take fresh raw milk with protein content of 3.5% (w / w), fat content of 4.2% (w / w), lactose content of 4.95% (w / w), ash content of 0.80%, and heat to 55 Centrifugal degreasing at ℃, centrifugal speed is 10000r / min, feed rate is 100L / h, adds sodium pyrophosphate and trisodium phosphate in above-mentioned skim milk, the proportioning of the two is 4: 1, and total addition is 0.5% ( w / w), stirred at 55°C for 10min, with a stirring rate of 100rpm, adjusted the pH value to 6.0 with 1mol / L HCl standard solution while stirring, and sterilized at 135°C for 4s;
[0051] 2) Use a 10kDa regenerated cellulose membrane to carry out ultrafiltration of the sterilized skim milk in step 1), the ultrafiltration temperature is 40°C, and the pressure is 3×10 5 Pa, the membrane flux in the ultrafiltration process is 30L m -2 h -1 , after adding deionized water, the membrane flux is 35L·m -2 h -1 ,specifically:
[0052] A. MPC40: Collect the retentate after ultrafiltration and concent...
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