Phyllachora dalbergiicola molecular detection primer and fast detecting method for dalbergia odorifera

Abstract

The invention belongs to the field of molecular detection of plant diseases, and particularly relates to a phyllachora dalbergiicola molecular detection primer and a fast detecting method for dalbergia odorifera. Nest PCR amplification, PCR product amplification analysis, agarose gel electrophoresis and result determination are carried out on a pair of designed phyllachora dalbergiicola specific primers of dalbergia odorifera. If phyllachora dalbergiicola exits in a sample, a target strip of 273 bp can be generated in sepharose gel under ultraviolet light; and if the strip does not appear, no phyllachora dalbergiicola exists. The primer is high in specificity, and the phyllachora dalbergiicola, phyllachora graminis, phyllachora phyllostachydis, and other pathogenic bacteria and endophytic fungi separated from dalbergia odorifera and pterocarpus macrocarpus can be distinguished. Moreover, the primer is high in sensitivity and can be used to detect a phyllachora dalbergiicola genome DNA with the lower limit of 100 ag/[mu]L. The detecting method is fast, and an accurate detecting result can be obtained within eight hours while a detecting result only can be obtained within at least four days or more than one week through a traditional disease diagnosis method.

Description

technical field [0001] The invention belongs to the field of molecular detection of plant diseases, and in particular relates to a molecular detection primer of Dalbergia serrata and a detection method thereof, which can be used for the early diagnosis of Dalbergia serrata and the monitoring and identification of pathogens. Background technique [0002] Dalbergia sinensis is one of the precious native tree species in the tropics in Hainan Province. It is a national second-class protected tree species. Among the 34 species of mahogany in 5 genera and 8 categories in our national standard, it is the mahogany second only to the red sandalwood. Its material is hard and heavy, strong, dry without cracking, deformation, and uniform in structure. [0003] With the increasing planting area of ​​Dalbergia japonica plantation in Hainan Province, the problem of pests and diseases has become increasingly prominent. Dalbergia mellifera disease is caused by the fungus Dalbergia melena, w...

AI Technical Summary

Problems solved by technology

Traditional plant disease diagnosis methods need to go through the process of pathogenic bacteria isolation, cultivation, identification, etc., which is time-consuming and heavy workload, and may miss the best period of disease control

And because the nevus fungus is a kind of obligate parasitic biotrophic fungus, that is, it cannot grow on the culture medium, so the pathogenic bacteria cannot be isolated by tissue separation method, which brings great difficulties to the accurate diagnosis of the disease

Images