Micro-fluidic chip used for raw milk body cell counting

Abstract

The invention discloses a micro-fluidic chip used for raw milk body cell counting. The micro-fluidic chip comprises a micro-fluidic channel unit plate arranged on a substrate, wherein a group of micro-fluidic channels for bearing a measured raw milk sample fluid is formed on the micro-fluidic channel unit. Each micro-fluidic channel comprises an inlet for injecting a pre-treated to-be-measured raw milk sample fluid, 2-6 channels which are connected on the inlet at certain ends and are communicated with the inlet, wherein each of the other ends of the 2-6 channels is connected with a fluid collecting cavity for collecting a detected waste liquid; 2-6 micro cavities which are communicated with the channel for placing the to-be-measured raw milk sample fluid are formed in each channel at equal intervals. The distribution, in the micro cavities, of the body cells is limited by the height of the micro cavities, so that the body cells within the field of view are guaranteed to be almost in a focal plane at the same height, and thus, focusing in an imaging process is facilitated to form a clear microscopic image, and accurate counting is realized. The micro-fluidic chip can be directly applied to counting raw milk body cells by a microscopic method, and can be used for remarkably improving the detection precision.

Description

technical field [0001] The invention relates to a microfluidic chip. In particular, it relates to a microfluidic chip for raw milk somatic cell counting applied to direct microscopy. Background technique [0002] Milk somatic cell count (SCC) refers to the total number of cells per milliliter of milk, most of which are white blood cells, accounting for about 95% of the somatic cell count. The somatic cell count reflects the quality of milk and the health of cows. Under normal circumstances, the number of somatic cells in milk is generally 20,000 to 200,000 / mL. If its content exceeds 500,000 / mL, the cows will show clinical symptoms of mastitis. When the number of somatic cells is between 200,000 and 500,000 / mL, cows often suffer from invisible mastitis, which is difficult to detect in terms of symptoms, but seriously affects the quality and taste of fresh milk. The current somatic cell counting methods for fresh milk include traditional detection methods such as direct mic...

AI Technical Summary

Problems solved by technology

However, the height of the shallow dish container in this design is difficult to ensure the contour distribution of the somatic cells to be detected in the height direction, which is not conducive to accurate focusing in the process of direct microscope inspection, thus affecting the accuracy of the detection results

Chinese application 201220232862.3 (milk somatic cell 4-hole detection test paper) provides a sandwich-structured paper-based detection test paper for milk somatic cell detection, but the color depth of this method depends on the reaction substrate and corresponding reaction enzyme on the test paper , and the content of somatic cells must be estimated with the help of a spectrophotometer, which is an indirect test method, which affects the reliability of the test results

Images