Method for directly extracting fatty acid methyl ester from microalgae wet biomass
A fatty acid methyl ester and biomass technology, applied in the direction of fatty acid esterification, fat generation, fatty acid production, etc., can solve the problem of high product cost, achieve the effect of scientific design, guaranteed yield, and improved simplicity
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Embodiment 1
[0029] Centrifuge at 4000rpm for 10min to collect cells at a density of 10 7 1 / mL Phaeodactylum tricornutum algae liquid 100 mL, after removing the supernatant to obtain about 0.1 g of microalgae wet biomass, its water content was determined to be 88%, add 0.5 mL of n-hexane and 1 mL of mass fraction to the centrifuge tube 2% methanol solution of sodium hydroxide, transferred to a sealed test tube, heated to 80°C, kept at a constant temperature for 20 minutes, cooled to room temperature for 5 minutes after the reaction, then added 1 mL of 10% acetyl chloride methanol solution, heated to 80 ℃, keep the temperature for 20 minutes, cool to room temperature for 5 minutes after the reaction, add 0.5mL of n-hexane and 1mL of potassium carbonate solution with a mass fraction of 6%, oscillate and stand still, and transfer all the upper liquid to a rotary evaporator after stratification occurs. Heat to 68°C, distill the n-hexane, and the remaining liquid is fatty acid methyl ester. Acc...
Embodiment 2
[0031] Centrifuge at 4500rpm for 10min to collect cells at a density of 10 7 100 mL of Isochrysis globosa algae solution per mL, about 0.1 g of microalgae wet biomass was obtained after removing the supernatant, and its water content was determined to be 90%. Add 0.5 mL of n-hexane and 1 mL of mass fraction to the centrifuge tube 2% sodium hydroxide methanol solution, transferred to a sealed test tube, heated to 80°C, kept at a constant temperature for 20 minutes, cooled to room temperature for 5 minutes after the reaction, then added 1mL of 10% acetyl chloride methanol solution, heated to 80°C , keep the temperature for 20min, cool down to room temperature for 5min after the reaction, add 0.5mL of n-hexane and 1mL of potassium carbonate solution with a mass fraction of 6%, oscillate and stand still, after stratification occurs, transfer all the upper liquid to a rotary evaporator, heat Distill the n-hexane to 68°C, and the remaining liquid is fatty acid methyl ester. As deter...
Embodiment 3
[0033] In this embodiment, glyceryl heptadecanoate is used as the test sample. Most of the fatty acids in microalgae exist in the form of triacylglycerides, and glyceryl heptadecanoate is one of them. Therefore, by measuring glyceryl heptadecanoate The conversion rate that ester is converted into methyl heptadecanoate can further prove the accuracy of the present invention. Take 0.5 mL of glyceryl heptadecanoate in toluene (containing 0.1 mg of glyceryl heptadecanoate, equivalent to about 0.1 mg of methyl heptadecanoate) in a test tube, add 0.5 mL of n-hexane and 1 mL of mass Sodium hydroxide methanol solution with a fraction of 2%, heat to 80°C after sealing, keep the temperature for 20min, cool to room temperature for 5min after the reaction, then add 1mL acetyl chloride methanol solution with a mass fraction of 10%, heat to 80°C, keep the temperature for 20min After the reaction, cool down to room temperature for 5 minutes, add 0.5 mL of methyl nonadecanoate n-hexane soluti...
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